Carm1 (BC036974) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC46828
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209145 representing BC036974 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGCGGCAGCGACGGCGGTGGGGCCGGGTGCGGGGAGCGCTGGGGTGGCGGGCCCGGGCGGCG CGGGGCCCTGCGCTACAGTGTCTGTGTTCCCGGGCGCCCGCCTCCTCACTATCGGCGACGCGAACGGCGA GATCCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTTGAGGTGCGCGCCGGACCAGACGCGGCGGGCATC GCCCTCTACAGCCATGAAGATGTGTGTGTTTTCAAGTGCTCGGTGTCCCGAGAGACAGAGTGCAGTCGTG TGGGCAGACAGTCCTTCATCATCACCCTGGGCTGCAACAGCGTCCTCATCCAGTTTGCCACACCCCACGA TTTCTGTTCTTTCTACAACATCCTGAAAACCTGTCGGGGCCACACACTGGAGCGCTCTGTGTTCAGTGAG CGGACAGAGGAATCCTCAGCTGTGCAGTACTTCCAGTTCTATGGCTACCTATCCCAGCAGCAGAACATGA TGCAGGACTATGTGCGGACAGGCACCTACCAGCGTGCGATCCTGCAGAACCACACGGACTTCAAGGACAA GATCGTTCTAGATGTGGGCTGTGGCTCTGGGATCCTGTCATTTTTTGCTGCTCAAGCAGGAGCCAGGAAA ATTTATGCAGTGGAAGCCAGCACCATGGCTCAGCATGCAGAGGTCCTGGTGAAGAGTAACAATCTGACAG ACCGCATCGTGGTCATCCCTGGCAAAGTAGAGGAGGTCTCATTGCCTGAGCAAGTGGACATTATCATCTC AGAGCCCATGGGCTACATGCTCTTCAATGAACGAATGCTCGAGAGCTACCTCCATGCCAAAAAGTACCTG AAGCCTAGTGGAAACATGTTCCCCACCATTGGTGATGTCCACCTCGCACCCTTCACTGATGAACAGCTCT ACATGGAGCAGTTCACCAAAGCCAACTTCTGGTACCAGCCATCCTTCCATGGAGTGGACCTGTCGGCCCT CAGAGGTGCCGCTGTGGATGAGTACTTCCGGCAACCTGTGGTGGACACATTTGACATCCGGATCCTGATG GCCAAATCTGTCAAGTACACAGTGAACTTCTTAGAAGCCAAAGAAGGCGATTTGCACAGGATAGAAATCC CATTCAAATTCCACATGCTGCATTCAGGGCTAGTCCATGGCTTGGCCTTCTGGTTCGATGTTGCTTTCAT TGGCTCCATAATGACCGTGTGGCTATCCACAGCCCCAACAGAGCCCCTGACCCACTGGTACCAGGTCCGG TGCCTCTTCCAGTCACCGTTGTTTGCCAAGGCCGGGGACACGCTCTCAGGGACATGTCTGCTTATTGCCA ACAAAAGACAGAGCTATGACATCAGTATTGTGGCACAGGTGGACCAGACAGGCTCCAAGTCCAGTAACCT GCTGGATCTAAAGAACCCCTTCTTCAGGTACACAGGTACAACCCCATCACCCCCACCTGGCTCACACTAC ACGTCTCCCTCGGAGAATATGTGGAACACAGGAAGCACCTATAATCTCAGCAGCGGGGTGGCTGTGGCTG GAATGCCTACTGCCTACGACCTGAGCAGTGTTATTGCCGGCGGCTCCAGTGTGGGTCACAACAACCTGAT TCCCTTAGGCTCCTCAGGTGCCCAGGGAGGCGGCGGTAGCTCCAGTGCCCACTATGCAGTCAACAACCAG TTCACCATGGGTGGCCCTGCCATCTCTATGGCCTCGCCCATGTCCATCCCGACCAACACCATGCACTATG GGAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209145 representing BC036974 Red=Cloning site Green=Tags(s) MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGI ALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSE RTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARK IYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYL KPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILM AKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVR CLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHY TSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLGSSGAQGGGGSSSAHYAVNNQ FTMGGPAISMASPMSIPTNTMHYGS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC036974
ORF Size	1757 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC036974, AAH36974
RefSeq Size	3171 bp
RefSeq ORF	1757 bp
Locus ID	59035
Gene Summary	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.[UniProtKB/Swiss-Prot Function]

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