Ubqln1 (BC026847) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	DA41, Dsk2, XDRP1, Plic-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209113 representing BC026847 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGAGCGCAGAGAGCGGCGGCCCACCGGGCGCGCAGGACAGTGCGGCCGACGGCGGCCCCGCAG AGCCCAAGATCATGAAGGTCACGGTGAAGACGCCTAAAGAGAAGGAGGAGTTTGCCGTGCCCGAGAACAG CTCGGTCCAGCAGTTCAAAGAGGAAATCTCTAAACGTTTCAAATCTCATATTGACCAACTTGTGTTGATA TTTGCTGGAAAAATTTTAAAAGATCAAGATACTCTGAGTCAGCATGGGATTCATGATGGACTTACGGTTC ACCTTGTCATCAAAACACAGAACAGGCCGCAAGATAATTCAGCTCAGCAAACAAATGCCCCTGGAAGCAC TGTGACCAGCTCACCAGCTCCCGATAGCAACCCCACGTCTGGTTCTGCTGCTAACAGCTCCTTTGGTGTA GGCGGACTTGGAGGACTTGCAGGTCTTAGCAGCTTGGGTTTAAACACCACCAACTTCTCTGAACTCCAGA GCCAAATGCAGCGGCAACTTTTGTCTAACCCTGAAATGATGGTCCAGATCATGGAAAATCCCTTTGTCCA GAGCATGCTCTCAAATCCTGACCTGATGCGGCAATTGATTATGGCCAATCCACAGATGCAACAGTTGATA CAGAGAAATCCAGAGATTAGTCATATGCTTAATAATCCAGATATAATGAGACAAACACTGGAACTTGCGA GAAATCCAGCGATGATGCAGGAAATGATGAGAAACCAGGACCGAGCCTTGAGCAACCTAGAAAGTATTCC TGGGGGCTATAATGCCTTACGGCGCATGTACACCGATATCCAGGAGCCTATGCTGAATGCTGCGCAAGAA CAGTTTGGTGGTAATCCATTTGCGTCCTTAGTGAGCAGTTCGTCCTCAGCAGAAGGGACTCAGCCTTCTC GAACAGAAAATAGGGATCCGCTCCCCAACCCGTGGGCTCCGCAGACTTCCCAGAGCTCACCAGCCTCCGG TACCACTGGCAGCACCACTAACACCATGAGCACCTCTGGTGGCACTGCCACCAGTACCCCTGCTGGACAG AGCACCTCGGGCCCAAGCTTGGTGCCTGGCGCAGGAGCTAGTATGTTCAACACCCCCGGAATGCAGAGCC TGCTGCAGCAGATAACTGAGAACCCACAGCTCATGCAGAACATGCTGTCCGCCCCATACATGAGGAGCAT GCTGCAGTCCCTGAGCCAGAACCCCGACCTTGCTGCGCAGATGATGCTGAATAATCCCCTATTTGCTGGA AATCCTCAGCTTCAAGAACAAATGAGACAACAGCTCCCAACTTTCCTCCAACAAATGCAGAACCCCGACA CGCTCTCAGCGATGTCGAACCCTAGAGCCATGCAGGCGCTGCTGCAGATCCAGCAGGGCTTGCAGACGCT GGCCACAGAAGCTCCCGGCCTCATCCCAGGGTTTACTCCTGGCTTGGCAGCAGGCAATTCTGGAGGCTCT TCCGGAACCAATGCACCTAGCACTGCACCTAGTGAAGATACGAACCCCCAAGGGGGCACTGCTGAGCCAG GCCACCAGCAGTTTATCCAGCAGATGCTGCAGGCCCTTGCGGGGGTGAATCCTCAGCTGCAGAGTCCAGA AGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCCATGGGATTCTTGAACCGTGAAGCAAACCTGCAA GCTCTGATAGCCACAGGGGGCGACATCAATGCAGCAATCGAAAGGTTGCTGGGCTCCCAGCCGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209113 representing BC026847 Red=Cloning site Green=Tags(s) MAESAESGGPPGAQDSAADGGPAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHIDQLVLI FAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDNSAQQTNAPGSTVTSSPAPDSNPTSGSAANSSFGV GGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLI QRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLNAAQE QFGGNPFASLVSSSSSAEGTQPSRTENRDPLPNPWAPQTSQSSPASGTTGSTTNTMSTSGGTATSTPAGQ STSGPSLVPGAGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMLQSLSQNPDLAAQMMLNNPLFAG NPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLAAGNSGGS SGTNAPSTAPSEDTNPQGGTAEPGHQQFIQQMLQALAGVNPQLQSPEVRFQQQLEQLSAMGFLNREANLQ ALIATGGDINAAIERLLGSQPS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026847
ORF Size	1748 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026847, AAH26847
RefSeq Size	3687 bp
RefSeq ORF	1748 bp
Locus ID	56085
Gene Summary	Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Plays a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway. Plays a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis. Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently downregulating the ORAI1-mediated Ca2+ mobilization. Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing (By similarity). Links CD47 to the cytoskeleton (PubMed:10549293).[UniProtKB/Swiss-Prot Function]

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