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Me1 (NM_008615) Mouse Tagged ORF Clone
CAT＃: MG209005
- TrueORF®
Me1 (tGFP-tagged) - Mouse malic enzyme, supernatant (Mod1)
ORF Plasmid: DDK
"NM_008615" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D9Ertd267e; Mdh-1; Mod-1; Mod1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209005 representing NM_008615
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCGAGCCCCCCGCCGCCGACACACCCACCAGCGCGGCTACCTGCTGACGCGGGACCCGCATC TCAACAAGGACTTGGCTTTTACTCTGGAAGAGAGACAGCAATTGAACATTCATGGATTGTTGCCGCCCTG CATCGTCAGCCAGGAGCTCCAGGTCCTTAGAATAATTAAGAATTTCGAGCGACTGAACTCTGACTTCGAC AGGTATCTTCTGTTAATGGACCTGCAAGACAGAAATGAGAAGCTCTTCTACAGCGTGCTCATGTCTGATG TTGAAAAGTTCATGCCTATTGTTTACACCCCCACCGTGGGCCTTGCATGCCAGCAGTACAGTTTGGCATT CCGGAAGCCAAGAGGCCTCTTTATTAGTATCCATGACAAAGGGCACATTGCTTCAGTTCTTAATGCATGG CCAGAGGATGTCGTTAAGGCTATTGTGGTAACTGACGGAGAGCGCATCCTTGGCTTGGGAGACCTTGGCT GTAATGGGATGGGCATCCCTGTGGGTAAACTGGCCCTGTACACGGCATGTGGAGGGGTGAACCCACAACA GTGTCTACCCATCACTTTGGATGTGGGAACAGAAAATGAGGAGTTACTTAAGGATCCACTGTACATCGGG CTGCGGCACCGGCGAGTCAGAGGCCCTGAGTATGACGCCTTCCTGGATGAGTTCATGGAGGCAGCGTCTT CCAAATATGGCATGAATTGCCTTATTCAGTTTGAAGATTTTGCCAATCGGAATGCATTTCGTCTCCTGAA CAAGTATCGAAACAAGTATTGCACATTTAACGATGATATTCAAGCGTCTGTTGCGGTTGCGGGTCTCCTT GCAGCTCTTCGAATAACCAAGAACAAGCTCTCTGATCAGACAGTGCTGTTCCAGGGAGCTGGAGAGGCTG CCTTGGGGATTGCTCACTTGGTTGTTATGGCCATGGAGAAAGAAGGTTTATCAAAGGAGAATGCTAGAAA GAAGATATGGTTGGTTGACTCAAAAGGACTAATAGTTAAGGGTCGGGCTTCTCTCACAGAAGAGAAAGAG GTGTTTGCCCATGAACATGAAGAAATGAAGAATCTGGAAGCCATTGTTCAAAAGATAAAACCAACTGCCC TCATAGGAGTTGCTGCAATTGGTGGTGCTTTCACTGAACAAATTCTCAAGGATATGGCTGCCTTCAACGA GCGGCCCATCATCTTTGCTTTGAGCAATCCGACCAGCAAAGCGGAGTGCTCTGCAGAGCAGTGCTACAAG GTGACCAAGGGACGTGCAATCTTTGCCAGCGGCAGTCCTTTTGATCCAGTCACTCTCCCAGATGGACGGA CTCTGTTTCCTGGCCAAGGCAACAATTCCTACGTGTTCCCTGGAGTTGCTCTTGGGGTGGTGGCCTGCGG ACTGAGACACATCGATGATAAGGTCTTCCTCACCACTGCTGAGGTCATATCTCAGCAAGTGTCAGATAAA CACCTGCAAGAAGGCCGGCTCTATCCTCCTTTGAATACCATTCGAGACGTTTCGTTGAAAATTGCAGTAA AGATTGTGCAAGATGCATACAAAGAAAAGATGGCCACTGTTTATCCTGAACCCCAAAACAAAGAAGAATT TGTCTCCTCCCAGATGTACAGCACTAATTATGACCAGATCCTACCTGATTGTTATCCGTGGCCTGCAGAA GTCCAGAAAATACAGACCAAAGTCAACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209005 representing NM_008615
Red=Cloning site Green=Tags(s) MEPRAPRRRHTHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIVSQELQVLRIIKNFERLNSDFD RYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAW PEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIG LRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQASVAVAGLL AALRITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEKEGLSKENARKKIWLVDSKGLIVKGRASLTEEKE VFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYK VTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDK HLQEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWPAE VQKIQTKVNQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008615 |
| ORF Size | 1716 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008615.1, NP_032641.1 |
| RefSeq Size | 3129 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 17436 |
| UniProt ID | P06801 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200950 | Me1 (untagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1, (10ug) |
CNY 4,264.00 |
|
| MR209005 | Me1 (Myc-DDK-tagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1 |
CNY 3,656.00 |
|
| MR209005L3 | Lenti ORF clone of Me1 (Myc-DDK-tagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1 |
CNY 6,460.00 |
|
| MR209005L4 | Lenti ORF clone of Me1 (mGFP-tagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 1 |
CNY 6,460.00 |
