Nfib (BC014290) Mouse Tagged ORF Clone

CAT#: MG208973

  • TrueORF®

Nfib (tGFP-tagged) - Mouse nuclear factor I/B (cDNA clone MGC:13959 IMAGE:4038233)



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Mouse Protein / Lysate Request

CNY 8,392.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 6720429L07Rik; CTF; E030026I10Rik; NF-I/B; NF1-B; NFI-B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208973 representing BC014290
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTCCACCCATTTATTGAGGCACTTCTTCCTCACG
TCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAA
ACGAATGTCGAAGGATGAAGAAAGGGCAGTCAAAGACGAGCTGCTCAGTGAGAAGCCCGAAATCAAGCAG
AAGTGGGCATCCAGGCTCCTGGCCAAACTGCGCAAAGATATCCGCCAGGAGTACCGGGAGGACTTTGTGC
TTACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCAGACCAGAAGGGTAAGATTAGGAG
GATCGACTGCCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC
ATCCCTTTGGAGAGTACGGATGGAGAGCGACTCATGAAGTCCCCGCACTGCACAAACCCAGCACTTTGTG
TTCAGCCACACCACATCACAGTATCAGTTAAGGAGCTTGACTTGTTTTTGGCATACTACGTGCAGGAGCA
AGATTCTGGACAATCAGGAAGTCCAAGCCACAGTGATCCTGCCAAGAATCCTCCAGGGTACCTCGAGGAC
AGCTTTGTAAAATCCGGAGTCTTCAATGTATCAGAGCTTGTGAGAGTATCCAGAACACCCATAACCCAGG
GAACTGGAGTCAACTTCCCAATCGGAGAAATTCCCAGCCAACCATACTATCATGACATGAACTCTGGTGT
GAACCTGCAGAGGTCGCTGTCTTCTCCACCGAGCAGCAAAAGACCCAAAACTATATCTATAGATGAAAAT
ATGGAGCCAAGTCCTACAGGAGACTTTTACCCCTCTCCAAATTCACCAGCTGCTGGAAGTCGAACATGGC
ATGAACGAGATCAAGATATGTCTTCTCCAACTACAATGAAGAAGCCTGAGAAGCCACTGTTTAGCTCTAC
ATCTCCACAGGATTCTTCCCCAAGATTGAGCACTTTCCCCCAGCACCATCATCCCGGAATACCTGGAGTC
GCGCACAGTGTCATCTCAACTCGAACTCCACCTCCGCCCTCACCGTTGCCATTTCCGACGCAAGCTATCC
TTCCTCCGGCACCTTCCAGCTACTTCTCTCATCCAACAATCAGATATCCTCCTCACCTGAATCCTCAGGA
TACTCTGAAGAACTACGTACCTTCTTATGACCCATCCAGTCCTCAAACGAGCCAGCCTAACAGCAGTGGT
CAAGTAGTAGGGAAAGTGCCTGGCCATTTCACACCTGTCTTGGCACCCTCTCCCCATCCCAGTGCAGTGC
GACCTGTGACCCTGACCATGACAGATACTAAACCCATCACTACATCCACTGAAGGTGAGGCAGCTTCACC
TACAGCAACCACCTACACAGCCTCAGGCACATCTCAAGCCAATCGATATGTGGGACTAAGCCCAAGAGAC
CCATCCTTCCTGCATCAGCAACAGCTGAGGATTTGTGACTGGACCATGAATCAAAACGGCAGGCATTTAT
ACCCCAGTACCAGTGAGGATACATTGGGAATTACTTGGCAAAGTCCTGGTACCTGGGCTAGCTTGGTTCC
TTTTCAAGTGTCAAATAGGACACCCATCTTACCGGCAAATGTCCAAAATTATGGTTTGAACATAATTGGA
GAGCCTTTCCTTCAAGCGGAGACAAGCAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208973 representing BC014290
Red=Cloning site Green=Tags(s)

MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQ
KWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKG
IPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHSDPAKNPPGYLED
SFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDEN
MEPSPTGDFYPSPNSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSTSPQDSSPRLSTFPQHHHPGIPGV
AHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQPNSSG
QVVGKVPGHFTPVLAPSPHPSAVRPVTLTMTDTKPITTSTEGEAASPTATTYTASGTSQANRYVGLSPRD
PSFLHQQQLRICDWTMNQNGRHLYPSTSEDTLGITWQSPGTWASLVPFQVSNRTPILPANVQNYGLNIIG
EPFLQAETSN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC014290
ORF Size 1710 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC014290, AAH14290
RefSeq Size 2386 bp
RefSeq ORF 1712 bp
Locus ID 18028
Gene Summary Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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