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Tas1r1 (BC070448) Mouse Tagged ORF Clone
CAT＃: MG208887
- TrueORF®
Tas1r1 (tGFP-tagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268)
ORF Plasmid: DDK
"BC070448" in other vectors (4)
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CNY 6,180.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TR1, T1r1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208887 representing BC070448
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTCTGGGCAGCTCACCTGCTGCTCAGCCTGCAGCTGGCCGTTGCTTACTGCTGGGCTTTCAGCT GCCAAAGGACAGAATCCTCTCCAGGTTTCAGCCTCCCTGGGGACTTCCTCCTGGCAGGCCTGTTCTCCCT CCATGCTGACTGTCTGCAGGTGAGACACAGACCTCTGGTGACAAGTTGTGACAGGTCTGACAGCTTCAAC GGCCATGGCTATCACCTCTTCCAAGCCATGCGGTTCACCGTTGAGGAGATAAACAACTCCACAGCTCTGC TTCCCAACATCACCCTGGGGTATGAACTGTATGACGTGTGCTCAGAGTCTTCCAATGTCTATGCCACCCT GAGGGTGCTCGCCCAGCAAGGGACAGGCCACCTAGAGATGCAGAGAGATCTTCGCAACCACTCCTCCAAG GTGGTGGCACTCATTGGGCCTGATAACACTGACCACGCTGTCACCACTGCTGCCCTGCTGAGCCCTTTTC TGATGCCCCTGGTCAGCTATGAGGCGAGCAGCGTGATCCTCAGTGGGAAGCGCAAGTTCCCGTCCTTCTT GCGCACCATCCCCAGCGATAAGTACCAGGTGGAAGTCATAGTGCGGCTGCTGCAGAGCTTCGGCTGGGTC TGGATCTCGCTCGTTGGCAGCTATGGTGACTACGGGCAGCTGGGCGTACAGGCGCTGGAGGAGCTGGCCA CTCCACGGGGCATCTGCGTCGCCTTCAAGGACGTGGTGCCTCTCTCCGCCCAGGCGGGTGACCCAAGGAT GCAGCGCATGATGCTGCGTCTGGCTCGAGCCAGGACCACCGTGGTCGTGGTCTTCTCTAACCGGCACCTG GCTGGAGTGTTCTTCAGGTCTGTGGTGCTGGCCAACCTGACTGGCAAAGTGTGGATCGCCTCCGAAGACT GGGCCATCTCCACGTACATCACCAATGTGCCCGGGATCCAGGGCATTGGGACGGTGCTGGGGGTGGCCAT CCAGCAGAGACAAGTCCCTGGCCTGAAGGAGTTTGAAGAGTCCTATGTCCAGGCAGTGATGGGTGCTCCC AGAACTTGCCCAGAGGGGTCCTGGTGCGGCACTAACCAGCTGTGCAGGGAGTGTCACGCTTTCACGACAT GGAACATGCCCGAGCTTGGAGCCTTCTCCATGAGCGCTGCCTACAATGTGTATGAGGCTGTGTATGCTGT GGCCCACGGCCTCCACCAGCTCCTGGGATGTACCTCTGGGACCTGTGCCAGAGGCCCAGTCTACCCCTGG CAGCTTCTTCAGCAGATCTACAAGGTGAATTTCCTTCTACATAAGAAGACTGTAGCATTCGATGACAAGG GGGACCCTCTAGGTTATTATGACATCATCGCCTGGGACTGGAATGGACCTGAATGGACCTTTGAGGTCAT TGGTTCTGCCTCACTGTCTCCAGTTCATCTAGACATAAATAAGACAAAAATCCAGTGGCACGGGAAGAAC AATCAGGTAATGGGACAAGGTCGCTCACCAGGTGACTGCTTTGTTACCAGAATCTGGGGAGTGATGCGAA CCCAGAGCAGGATGGGGCCCCACACATCAGGCTACCTCAGGTCTGCAGTCCTGCCAGGGATGCCAGCTGC AGTGTTGTCAAACAGATGTCGGAGAGGGGACCTGGAGGGCAGTGGCACAGATCCCTCTTCTCAGTCTCCC CTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208887 representing BC070448
Red=Cloning site Green=Tags(s) MLFWAAHLLLSLQLAVAYCWAFSCQRTESSPGFSLPGDFLLAGLFSLHADCLQVRHRPLVTSCDRSDSFN GHGYHLFQAMRFTVEEINNSTALLPNITLGYELYDVCSESSNVYATLRVLAQQGTGHLEMQRDLRNHSSK VVALIGPDNTDHAVTTAALLSPFLMPLVSYEASSVILSGKRKFPSFLRTIPSDKYQVEVIVRLLQSFGWV WISLVGSYGDYGQLGVQALEELATPRGICVAFKDVVPLSAQAGDPRMQRMMLRLARARTTVVVVFSNRHL AGVFFRSVVLANLTGKVWIASEDWAISTYITNVPGIQGIGTVLGVAIQQRQVPGLKEFEESYVQAVMGAP RTCPEGSWCGTNQLCRECHAFTTWNMPELGAFSMSAAYNVYEAVYAVAHGLHQLLGCTSGTCARGPVYPW QLLQQIYKVNFLLHKKTVAFDDKGDPLGYYDIIAWDWNGPEWTFEVIGSASLSPVHLDINKTKIQWHGKN NQVMGQGRSPGDCFVTRIWGVMRTQSRMGPHTSGYLRSAVLPGMPAAVLSNRCRRGDLEGSGTDPSSQSP L TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC070448 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC070448 |
| RefSeq Size | 4723 bp |
| RefSeq ORF | 1685 bp |
| Locus ID | 110326 |
| Gene Summary | Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218536 | Tas1r1 (untagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268), (10ug) |
CNY 6,940.00 |
|
| MR208887 | Tas1r1 (Myc-DDK-tagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268) |
CNY 5,610.00 |
|
| MR208887L3 | Lenti ORF clone of Tas1r1 (Myc-DDK-tagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268) |
CNY 7,510.00 |
|
| MR208887L4 | Lenti ORF clone of Tas1r1 (mGFP-tagged) - Mouse taste receptor, type 1, member 1 (cDNA clone MGC:99408 IMAGE:30619268) |
CNY 7,510.00 |
