Zfp354c (BC079908) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Tcf17l2, Kid3, AJ18, mKIAA4218
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208882 representing BC079908 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGATTTGCTGGCTGCCCGAGGCACAGAGCCTGTGACCTTCAGGGATGTGGCCGTGTCCTTCA GCCAGGATGAGTGGTTGCACCTGGACCCTGCTCAGAGAAGCCTGTACCGAGAGGTGATGCTGGAGAACTA CAGCAACCTGGCCTCGCTGGGGTTTCAGGCTTCAATACCTCCTGTGATTGGCAAGCTGCAAAAAGGGCAA GATCCCTGCATGGAAAGAGAAGCCCCTGAAGACACCTGCCTAGATTTCGAGATTTGGCCAGAGATAGAAG CATTGCCACCCAAACAAGATGTTCTTACTAAAGAGACATCACATGGGCTGATAAAGAATGGATCCACTAA GTGTGTTTACTGGAAAATCAGCTTTGGAGAGCTTGTGAAAACTGAGTGCCGAGATATAGCACAGGAGCAA GAAAAGAAAGTGCATGGGCCAGGGGCAGAGTCACCCAAGGAAACCACCAGTGAAGACGGAACTCCCACGG GCTTCGAGCCGGAGAAACCCTTGTTCATTAGTAAAGCACTTGTCTCACAAGAGGGTGATCCTACAGAAAG TGTTCCTGCTACGTATCACACTTCAGAGAAAGATTTACCACAGGATTTTGATCTAATGAGAAGCTTTCAA ATGTACCCAGGACAAAAACCTCATGTCTGCAGTGAATGTGGCAAGGGTTTCACCCAAAGCCTTCATCTTC TAGAGCACAAGAGGCTTCACACTGGGGAGAAGCCGTACAAATGCAGCGAGTGTGGGAAGAGCTTCAGCCA TAGATCCTCCCTCCTGGCTCACCAGAGAACCCACACCGGAGAGAAGCCGTACAAGTGCAGCGAGTGTGAG AAAGCCTTCGGCAGCAGCTCCACTCTCATCAAACACCTGAGGGTGCACACCGGAGAGAAACCTTACCGCT GCCGGCAGTGTGGGAAGGCCTTCAGCCAGTGTTCCACCCTCACTGTACACCAGAGGATTCACACTGGGGA AAAGCTCTATAAATGTGCCGAGTGTGACAAGGCCTTTAACTGCAGAGCAAAGCTCCACAGACATCAGAGA ATCCACACAGGGGAGAAGCCATATAAATGTGCTGAGTGTGGGAAGGGCTACAGTCAGTTTCCATCCCTAG CCGAACACCAGAGACTCCATACTGGCGAGCAGCTTTGCCAGTGTTTGCAATGTGGGAGAACCTTTACACG TGTGTCTACCCTTATCGAACACCAGAGAATTCATACTGGACAGAAACCATATCAATGCAACGAATGTGGG AAGACTTTCAACCAGTATTCCTCCTTCAATGAACATCGGAAGATTCATACCGGGGAGAAACTTTACACAT GTGAAGAATGTGGGAAAGCCTTTGGCTGTAAATCAAACCTTTATCGGCATCAGAGGATCCATACTGGAGA GAAACCCTATCAGTGTAACCAGTGTGGAAAGGCCTTCAGCCAGTATTCATTCCTAACTGAACACGAGCGA ATCCATACTGGAGAGAAGCTCTACAAGTGTATGGAGTGTGGGAAAGCCTATAGTTACAGGTCAAACCTTT GTAGACACAAAAAAGTCCACCTTAAGGAAAGGCTGTATAAATGGAAAGAATATGGGACACCCTTTATCTA TGGCTCATCCCTTACTCCATATCAGAAATTTCTGAAAGGAGACAAGCCTGAGAACTTTAATTCATCGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208882 representing BC079908 Red=Cloning site Green=Tags(s) MAVDLLAARGTEPVTFRDVAVSFSQDEWLHLDPAQRSLYREVMLENYSNLASLGFQASIPPVIGKLQKGQ DPCMEREAPEDTCLDFEIWPEIEALPPKQDVLTKETSHGLIKNGSTKCVYWKISFGELVKTECRDIAQEQ EKKVHGPGAESPKETTSEDGTPTGFEPEKPLFISKALVSQEGDPTESVPATYHTSEKDLPQDFDLMRSFQ MYPGQKPHVCSECGKGFTQSLHLLEHKRLHTGEKPYKCSECGKSFSHRSSLLAHQRTHTGEKPYKCSECE KAFGSSSTLIKHLRVHTGEKPYRCRQCGKAFSQCSTLTVHQRIHTGEKLYKCAECDKAFNCRAKLHRHQR IHTGEKPYKCAECGKGYSQFPSLAEHQRLHTGEQLCQCLQCGRTFTRVSTLIEHQRIHTGQKPYQCNECG KTFNQYSSFNEHRKIHTGEKLYTCEECGKAFGCKSNLYRHQRIHTGEKPYQCNQCGKAFSQYSFLTEHER IHTGEKLYKCMECGKAYSYRSNLCRHKKVHLKERLYKWKEYGTPFIYGSSLTPYQKFLKGDKPENFNSSL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079908
ORF Size	1682 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079908, AAH79908
RefSeq Size	5103 bp
RefSeq ORF	1682 bp
Locus ID	30944
Gene Summary	May function as a transcription repressor. Binds to 5'-CCACA-3' core sequence. Suppresses osteogenic effects of RUNX2. May be involved in osteoblastic differentiation (By similarity). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).[UniProtKB/Swiss-Prot Function]

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