Traf5 (BC012702) Mouse Tagged ORF Clone

CAT#: MG208836

  • TrueORF®

Traf5 (tGFP-tagged) - Mouse Tnf receptor-associated factor 5 (cDNA clone MGC:13966 IMAGE:4218625)



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Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208836 representing BC012702
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCATTCGGAGGAGCAAGCGGCTGTCCCCTGCGCCTTCATCCGCCAGAACTCTGGCAACTCAATTT
CCTTGGACTTTGAGCCCGACACCGAGTACCAGTTTGTGGAGCAGCTGGAAGAACGCTACAAATGTGCCTT
CTGCCACTCCGTGCTTCACAACCCCCACCAGACCGGCTGCGGGCACCGCTTCTGCCAGCAGTGCATCCGG
TCTCTGAGAGAATTGAACTCGGTGCCGATCTGCCCGGTAGACAAGGAGGTCATCAAGCCTCAGGAGGTGT
TCAAAGACAACTGCTGCAAAAGAGAAGTTCTCAATTTACACGTCTACTGCAAAAACGCCCCCGGGTGCAA
TGCCAGGATTATTCTGGGACGATTCCAGGACCACCTTCAGCACTGTTCCTTCCAAGCCGTGCCCTGCCCT
AACGAGAGCTGCCGGGAAGCCATGCTCCGGAAAGACGTGAAAGAGCACCTGAGCGCATACTGCCGGTTCC
GAGAGGAGAAGTGCCTTTACTGCAAAAGGGACATAGTGGTGACCAACCTGCAGGATCATGAGGAAAACTC
GTGTCCTGCGTACCCAGTGTCTTGTCCCAACAGGTGTGTGCAGACTATTCCAAGAGCTAGGGTGAATGAA
CACCTTACTGTATGTCCTGAGGCTGAGCAAGACTGTCCCTTTAAGCACTATGGCTGCACTGTCAAGGGTA
AGCGGGGGAACTTGCTGGAGCATGAGCGGGCAGCCCTGCAGGACCACATGCTTCTGGTTTTAGAGAAGAA
CTACCAACTAGAACAGCGGATCTCTGATTTATATCAGAGTCTCGAACAGAAGGAAAGCAAGATCCAGCAG
CTGGCAGAAACCGTGAAGAAGTTCGAAAAGGAGCTTAAGCAGTTCACACAGATGTTTGGCAGAAATGGAA
CTTTCCTCTCAAATGTCCAGGCTCTCACCAGTCACACGGACAAGTCAGCTTGGCTGGAAGCGCAGGTGCG
GCAGCTGCTACAAATAGTTAACCAGCAGCCAAGTCGACTTGATCTGAGGTCTTTGGTGGATGCGGTTGAC
AGCGTGAAACAGAGGATCACCCAGCTGGAAGCCAGTGACCAGAGATTAGTTCTTTTAGAGGGGGAGACCA
GCAAGCACGACGCACACATTAATATCCACAAAGCACAGCTGAATAAGAACGAAGAGCGGTTTAAGCAGCT
GGAGGGCGCCTGCTACAGTGGCAAGCTCATCTGGAAGGTGACAGATTACAGGGTGAAGAAGAGGGAGGCC
GTGGAGGGGCACACAGTGTCCGTCTTCAGCCAGCCTTTCTACACCAGCCGCTGCGGCTACCGGCTCTGTG
CCAGGGCGTACCTGAACGGGGACGGGTCGGGGAAGGGAACGCACCTGTCCCTGTACTTTGTGGTGATGCG
CGGTGAGTTTGACTCGCTGCTGCAGTGGCCGTTCAGGCAGAGGGTGACCCTGATGCTTTTGGACCAGAGC
GGCAAGAAGAACCATATTGTGGAGACCTTCAAAGCTGACCCCAACAGCAGCAGCTTCAAAAGGCCAGATG
GCGAGATGAACATTGCCTCTGGCTGTCCCCGCTTTGTGTCGCACTCTACTCTGGAGAACTCCAAGAACAC
CTACATTAAAGACGACACACTGTTCTTGAAAGTGGCCGTGGATTTAACTGACTTGGAGGATCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208836 representing BC012702
Red=Cloning site Green=Tags(s)

MAHSEEQAAVPCAFIRQNSGNSISLDFEPDTEYQFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIR
SLRELNSVPICPVDKEVIKPQEVFKDNCCKREVLNLHVYCKNAPGCNARIILGRFQDHLQHCSFQAVPCP
NESCREAMLRKDVKEHLSAYCRFREEKCLYCKRDIVVTNLQDHEENSCPAYPVSCPNRCVQTIPRARVNE
HLTVCPEAEQDCPFKHYGCTVKGKRGNLLEHERAALQDHMLLVLEKNYQLEQRISDLYQSLEQKESKIQQ
LAETVKKFEKELKQFTQMFGRNGTFLSNVQALTSHTDKSAWLEAQVRQLLQIVNQQPSRLDLRSLVDAVD
SVKQRITQLEASDQRLVLLEGETSKHDAHINIHKAQLNKNEERFKQLEGACYSGKLIWKVTDYRVKKREA
VEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQS
GKKNHIVETFKADPNSSSFKRPDGEMNIASGCPRFVSHSTLENSKNTYIKDDTLFLKVAVDLTDLEDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC012702
ORF Size 1676 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC012702, AAH12702
RefSeq Size 2216 bp
RefSeq ORF 1676 bp
Locus ID 22033
Gene Summary Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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