Ncaph2 (BC003900) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC6766
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208828 representing BC003900 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTCATTGAGGCAGCACTGTTGATCCAGGGCTCAGCCTGTGTCTACAGTAAGAAGGTGGAGTACC TCTACTCGCTGGTCTACCAGGCTCTCGATTTTATTTCTGGCAAGAGGCGGGCCAAACAGCTCTCCTTAGT TCAGGAAGATGGGAGCAAGAAGACTGTCAACTCAGAGACTCCCTGTGAAACAGAGAATGAGTTCCTGTCA CTGGACGACTTCCCTGACTCCCGGGCTAATGTGGATCTGAAAAATGATCAGGCATCCAGTGAGCTGCTTA TCATACCCCTACTGCCCATGGCCCTGGTGGCCCCTGATGAAGTGGAAAAGAACAGCAGCCCCTTGTATAG CTGTCAGGGTGACATCTTGGCCAGCCGGAAGGATTTCAGGATGAACACGTGTATGCCTAACCCCAGAGGC TGCTTTATGTTAGATCCAGTGGGAATGTGTCCTGTGGAGCCTGTGGTGCCCGTGGAGCCATACCCCATGT CAAGGAGCCAGAAAGATCCTGAGGACGCTGAGGAGCAGCCCATGGAAGTGTCTAGGAACGGGAGTCCTGT TCCTGTACCCGACATCTCCCAAGAGCCAGATGGTCCAGCGCTCAGCGGTGGAGAGGAGGATGCAGAGGAT GGAGCAGAGCCCCTGGAGGTTGCTCTAGAGCCTGCAGAGCCAAGGACCTCACAGCAGAGTGCCATCTTGC CAAGGAGATACATGCTGCGGGAACGACAAGGGGCACCGGAGCCTGCCTCCCGGCTACAGGAGACCCCAGA CCCCTGGCAGAGCCTGGACCCTTTTGACTCCTTGGAATCTAAGGTCTTCCAGAAAGGGAAACCCTATTCT GTGCCACCCGGTGTGGAGGAGGCTCCAGGACAGAAGCGCAAGAGGAAGGGTGCCACCAAGTTGCAGGACT TCCACAAGTGGTACCTGGATGCCTATGCTGAACACCCTGACGGCAGGAGGGCTCGGCGGAAGGGCCCAAC CTTTGCAGACATGGAAGTCCTGTACTGGAAACATGTGAAAGAACAGCTCGAGACCCTTCAGAAGCTGCGG AGACGTAAGATCAACGAGAGATGGCTACCTGGGGCCAAGCAGGATCTGTGGCCTACAGAGGAGGATCGCT TGGAAGAGTCCCTCGAGGACCTCGGGGTAGCAGATGACTTTCTAGAGCCCGAGGAGTACGTGGAGGAGCC TGCGGGGGTGATGCCCGAGGAAGCTGCTGACCTCGATGCAGAGGCCATGCCAGAGTCCCTGAGATACGAG GAGCTGGTCCGGAGAAATGTGGAACTCTTCATTGCCACCTCCCAGAAGTTTATCCAGGAGACAGAGCTGA GCCAACGCATCAGGGACTGGGAAGATACCATCCAGCCCCTGCTCCAGGAGCAGGAGCAGCATGTGCCCTT TGATATCCATATCTACGGGGACCAGTTGGCTTCACGGTTCCCCCAGCTCAATGAATGGTGTCCCTTTTCA GAGCTTGTAGCAGGGCAGCCTGCTTTTGAGGTGTGCCGCTCCATGCTGGCCTCCTTGCAACTGGCTAATG ACTACACAGTGGAGATCACTCAGCAGCCAGGACTGGAGGCAGCTGTGGACACAATGTCTCTGAGACTGCT CACACACCAGCGAGCCCACACCCGCTTCCAGACCTATGCTGCACCATCCATGGCCCAGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208828 representing BC003900 Red=Cloning site Green=Tags(s) MNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSLVQEDGSKKTVNSETPCETENEFLS LDDFPDSRANVDLKNDQASSELLIIPLLPMALVAPDEVEKNSSPLYSCQGDILASRKDFRMNTCMPNPRG CFMLDPVGMCPVEPVVPVEPYPMSRSQKDPEDAEEQPMEVSRNGSPVPVPDISQEPDGPALSGGEEDAED GAEPLEVALEPAEPRTSQQSAILPRRYMLRERQGAPEPASRLQETPDPWQSLDPFDSLESKVFQKGKPYS VPPGVEEAPGQKRKRKGATKLQDFHKWYLDAYAEHPDGRRARRKGPTFADMEVLYWKHVKEQLETLQKLR RRKINERWLPGAKQDLWPTEEDRLEESLEDLGVADDFLEPEEYVEEPAGVMPEEAADLDAEAMPESLRYE ELVRRNVELFIATSQKFIQETELSQRIRDWEDTIQPLLQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFS ELVAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC003900
ORF Size	1673 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003900, AAH03900
RefSeq Size	2063 bp
RefSeq ORF	1673 bp
Locus ID	52683
Gene Summary	This gene encodes a component of the condensin-2 complex. The encoded protein may regulate the structure of mitotic chromosomes. Loss of function of this gene disrupts T-cell development. There are two pseudogenes for this gene on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

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