Cdc14a (BC072644) Mouse Tagged ORF Clone
CAT#: MG208789
- TrueORF®
Cdc14a (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog A (S
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CNY 5,230.00
货期*
4周
规格
经常一起买 (5)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cdc14, CDC14A2, CDC14a1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208789 representing BC072644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGAGTCTGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA CTAAAATCATACAGTTTATCAAGAAAGAAGATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA ACGCAGCATTCTTGATAGGTGCTTATGCCGGATTACAGCATGGGTTTTTTGACTTTGAGACGTTTGATGC GGAAGAATATGAACATTATGAGCGAGTTGAAAACGGAGACTTCAACTGGATCGTTCCAGGAAAGTTTTTA GCGTTCAGTGGACCGCATCCTAAAAGCAAGATTGAGAATGGTTACCCTCTCCACGCTCCCGAAGCCTACT TTCCATACTTCAAAAAGAACAACGTGACCACGATCGTGAGATTGAACAAAAAGATCTACGAGGCGAAGCG CTTCACAGACGCTGGCTTCGAGCACTATGACCTGTTCTTCATAGACGGCAGCACCCCCAGCGATAACATC GTGCGGAGATTCCTGAACATCTGTGAGAACACCGAGGGGGCCATCGCGGTCCACTGCAAAGCTGGTCTGG GAAGAACCGGGACATTGATAGCCTGTTATGTCATGAAACACTACAGGTTTACACATGCTGAAATCATTGC TTGGATCAGAATTTGCCGACCAGGCTCCATCATCGGACCCCAGCAGCACTTCCTGAAAGAAAAACAAGCA TCATTGTGGGTCCAAGGAGACATTTTTAGATCCAAACTAAAAAATAGACCATCCAGTGAGGGAAGTATTA CAAAAATTATTTCTACCCTGGATGATATGTCTATTGGTGCAAATTTATCCAAATTACAAAGCACAGAAAG GATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAGAACAACGTAACGCAGGGAGACAAACTA CGTGCCTTAAAAAGCCAGAGGCATCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGATATGAAAG GACACCAAAGGGCAATGGCCCAGACTTTCAGATTAAGTTCCTCACCGCAACCAACTATGTCGACCATGAA GACCTCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAAAATAAGCCGAGGTTCTTTGTCTTCAGGA GCAAATATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAACGCCGGGACAG AAGAACCTGAGACCAAGAAGACCACATCACTCACCAAGGCCGCTTTCATAGCCAGCCCATTCACCAGCTT CTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAACCAGTACACCAGAAGC AGCAACAGCAACAGCAGCAGCAGCAGCAGCGGTCTTGGGGGCAACCTGAACAGCTCTCCAGTCCCCCAGA GCGCCAAGCCCGAGGAGCACACCACCATCCTTCGACCTTCCTTCCCGGGGTCACTCTCTTCCTCCTCAGT GAGATTCCTGAGCCGTTCTATACCTTCCCTTCAGTCTGAATACGTTCATTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208789 representing BC072644
Red=Cloning site Green=Tags(s) MAAESGELIGACEFMKDRLYFATLRNRPKSTINIHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK LKSYSLSRKKIVHYTSFDQRKRANAAFLIGAYAGLQHGFFDFETFDAEEYEHYERVENGDFNWIVPGKFL AFSGPHPKSKIENGYPLHAPEAYFPYFKKNNVTTIVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNI VRRFLNICENTEGAIAVHCKAGLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLKEKQA SLWVQGDIFRSKLKNRPSSEGSITKIISTLDDMSIGANLSKLQSTERIGENNFEDEDMEIKNNVTQGDKL RALKSQRHPRSSPSCAFRSDDMKGHQRAMAQTFRLSSSPQPTMSTMKTSKVCLSPSVTAKKISRGSLSSG ANIRSFSINSRLASSLGNLNAGTEEPETKKTTSLTKAAFIASPFTSFLNGSTQTPGRNYPELNNNQYTRS SNSNSSSSSSGLGGNLNSSPVPQSAKPEEHTTILRPSFPGSLSSSSVRFLSRSIPSLQSEYVHY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC072644 |
ORF Size | 1664 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC072644, AAH72644 |
RefSeq Size | 4319 bp |
RefSeq ORF | 1664 bp |
Locus ID | 229776 |
Gene Summary | Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity). Required for normal hearing (PubMed:29293958).[UniProtKB/Swiss-Prot Function] |
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