United States
Germany
Japan
United Kingdom
China
Cpsf6 (NM_001013391) Mouse Tagged ORF Clone
CAT＃: MG208746
- TrueORF®
Cpsf6 (tGFP-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6)
ORF Plasmid: DDK
"NM_001013391" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4733401N12Rik; AI256641; CFIM; CFIM68; HPBRII-4; HPBRII-7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208746 representing NM_001013391
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGGTGTGGACCACATAGACATTTACGCGGATGTGGGTGAAGAGTTCAACCAGGAAGCAGAAT ATGGTGGACATGATCAGATAGATTTGTATGATGATGTCATCTCTCCATCTGCAAATAATGGCGATGCCCC AGAAGATCGCGACTACATGGATACTCTTCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCCGCACCA AACGTTGTGTACACTTACACTGGGAAGAGAATCGCATTGTATATTGGAAATCTAACATGGTGGACAACAG ATGAGGACCTAACTGAGGCCGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTCTTTGAAAA TCGGGCAAATGGACAATCAAAGGGATTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCCAAAAAGTTA ATGGATCTTTTGCCTAAAAGAGAACTTCATGGTCAGAGTCCTGTTGTAACTCCATGCAATAAGCAGTTCC TGAGTCAATTTGAAATGCAATCCAGGAAAACTACACAGTCAGGACAGATGTCTGGGGAAGGGAAAGCCGG TCCTCCAGGAGGCGGTTCACGCGCAGCGTTTCCTCAAGGTGGTAGAGGACGGGGCCGGTTTCCAGGGGCT GTTCCTGGTGGGGACAGATTTCCTGGGCCAGCAGGACCAGGAGGGCCACCTCCACCTTTTCCAGCTGGAC AAACTCCACCACGTCCACCTTTAGGCCCACCTGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCA GGTTCTGCCACCTCCTCTAGCAGGACCTCCTAACCGAGGAGACCGCCCTCCACCACCAGTTCTTTTTCCT GGACAACCTTTTGGGCAGCCTCCGCTGGGTCCACTTCCTCCTGGGCCTCCACCTCCAGTTCCAGGCTACG GCCCCCCTCCAGGTCCACCGCCTCCACAGCAGGGACCACCTCCACCTCCAGGCCCCTTTCCACCTCGCCC ACCAGGTCCACTAGGGCCTCCCCTCACACTTGCTCCTCCTCCACATCTTCCGGGACCACCTCCAGGTGCC CCGCCACCAGCTCCACATGTGAATCCTGCTTTTTTTCCTCCACCAACTAATAGTGGCATGCCAACATCAG ATAGTCGAGGTCCACCACCAACAGACCCATATGGCCGACCTCCCCCATATGATAGGGGTGACTACGGTCC CCCTGGGAGGGAAATGGATACTGCAAGAACACCTCTGAGTGAAGCTGAGTTTGAAGAAATCATGAATAGA AATCGGGCAATCTCAAGCAGTGCTATTTCAAGAGCTGTGTCTGACGCTAGTGCTGGTGATTATGGGAGTG CTATTGAAACATTGGTAACAGCAATTTCTTTAATTAAACAATCCAAAGTCTCTGCAGATGATCGTTGCAA AGTTCTTATTAGCTCTCTGCAGGATTGCCTTCATGGAATCGAGTCCAAGTCCTATGGGTCTGGATCCAGA CGTGAACGATCAAGAGAACGGGACCATAGTAGATCACGGGAAAAGAGTCGTCGCCATAAATCTCGGAGTA GAGATCGCCACGATGACTATTACAGAGAGAGAAGCAGAGAACGAGAGAGACACCGGGATCGGGACCGCGA CCGTGACCGAGAGCGTGACCGAGAGCGAGAATACCGTCATCGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208746 representing NM_001013391
Red=Cloning site Green=Tags(s) MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAP NVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKL MDLLPKRELHGQSPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGGSRAAFPQGGRGRGRFPGA VPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFP GQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGA PPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNR NRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSR RERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013391 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013391.2 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 432508 |
| UniProt ID | Q6NVF9 |
| Gene Summary | Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202653 | Cpsf6 (untagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6), (10ug) |
CNY 4,112.00 |
|
| MR208746 | Cpsf6 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) |
CNY 4,112.00 |
|
| MR208746L3 | Lenti ORF clone of Cpsf6 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) |
CNY 6,460.00 |
|
| MR208746L4 | Lenti ORF clone of Cpsf6 (mGFP-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) |
CNY 6,460.00 |
