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Chek2 (NM_016681) Mouse Tagged ORF Clone
CAT＃: MG208692
- TrueORF®
Chek2 (tGFP-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2)
ORF Plasmid: DDK
"NM_016681" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cds1; CHK2; HUCDS1; Rad53 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208692 representing NM_016681
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGTCATCATCAGTCCCACAGCAGCACCTCCTCGAAGGCTCATGACAGTGCTTCCTGTTCACAGT CCCAGGGTGGCTTCAGCCAGCCCCAAGGCACTCCTTCTCAGTTGCACGAGCTCTCACAGTATCAGGGTTC GTCCAGCTCCTCTACCGGCACAGTGCCGTCCTCCAGCCAGTCCTCGCACTCCAGTTCTGGGACTCTGAGC TCGCTGGAGACAGTGTCTACCCAGGAACTCTGTTCTATTCCTGAGGACCAAGAACCTGAAGAACCTGGTC CTGCCCCTTGGGCTCGGCTATGGGCTCTTCAGGATGGATTCTCCAATCTAGACTGTGTTAACGACAACTA CTGGTTTGGGAGAGATAAAAGCTGTGAATATTGCTTCGATGGACCACTGTTGAGAAGGACGGACAAGTAC CGGACTTACAGCAAGAAGCATTTTCGTATTTTCAGGGAAATGGGCCCTAAAAATTGTTACATCGTATACA TAGAGGATCACAGTGGAAATGGAACCTTCGTAAATACCGAGCTTATTGGGAAAGGCAAACGCTGTCCTCT GAGTAACAACTCTGAAATCGCACTTTCACTATGTAGAAATAAAGTTTTTGTATTTTTTGATCTGACTGTA GATGATCAGTCAGTTTATCCTAAGGAATTAAGAGACGAATACATCATGTCAAAAACTCTTGGAAGTGGTG CGTGTGGGGAGGTAAAGATGGCTTTTGAGAGGAAGACATGTCAGAAAGTGGCCATAAAGATCATTAGCAA GCGGAGGTTTGCTCTTGGCTCATCGAGAGAAGCCGACACAGCTCCCAGTGTGGAAACTGAAATAGAAATT TTGAAGAAACTAAATCATCCATGCATCATCAAGATTAAAGATGTTTTTGATGCGGAAGATTATTACATTG TTCTGGAACTGATGGAAGGAGGAGAACTATTTGACCGGGTGGTGGGGAACAAGCGCCTGAAAGAAGCCAC CTGTAAGCTCTACTTCTACCAGATGCTTGTAGCTGTACAGTACCTTCACGAAAATGGGATCATACATCGG GACTTAAAGCCGGAGAATGTTCTTTTATCATCTCAGGAAGAGGATTGTCTAATCAAGATCACTGACTTTG GGCAGTCCAAGATCTTGGGGGAGACCTCCTTGATGAGAACCTTATGTGGTACGCCCACTTATCTGGCTCC TGAGGTTCTTGTCTCCAACGGGACTGCTGGGTACAGCCGCGCTGTGGACTGCTGGAGTTTAGGAGTTATT CTTTTCATCTGCCTAAGTGGGTATCCACCTTTCTCTGAGCATAAGACCCAAGTGTCCCTGAAGGATCAGA TCACCAGTGGAAAGTACAACTTTATTCCTGAAGTCTGGACAGATGTCTCAGAGGAGGCTCTGGACCTTGT CAAGAAACTGTTAGTTGTAGACCCAAAGGCTCGGCTTACCACAGAGGAGGCCTTAAATCATCCGTGGCTT CAGGATGAGTACATGAAGAAGAAATTTCAGGATCTCCTGGTGCAGGAAAAGAACTCGGTGACCCTCCCTG TGGCTCCCGCCCAGACTTCCAGTCAAAAGCGGCCCCTGGAACTGGAGGTGGAGGGTATGCCGAGCACAAA ACGCCTGTCTGTGTGTGGGGCTGTGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208692 representing NM_016681
Red=Cloning site Green=Tags(s) MKSHHQSHSSTSSKAHDSASCSQSQGGFSQPQGTPSQLHELSQYQGSSSSSTGTVPSSSQSSHSSSGTLS SLETVSTQELCSIPEDQEPEEPGPAPWARLWALQDGFSNLDCVNDNYWFGRDKSCEYCFDGPLLRRTDKY RTYSKKHFRIFREMGPKNCYIVYIEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTV DDQSVYPKELRDEYIMSKTLGSGACGEVKMAFERKTCQKVAIKIISKRRFALGSSREADTAPSVETEIEI LKKLNHPCIIKIKDVFDAEDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQMLVAVQYLHENGIIHR DLKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLVSNGTAGYSRAVDCWSLGVI LFICLSGYPPFSEHKTQVSLKDQITSGKYNFIPEVWTDVSEEALDLVKKLLVVDPKARLTTEEALNHPWL QDEYMKKKFQDLLVQEKNSVTLPVAPAQTSSQKRPLELEVEGMPSTKRLSVCGAVL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016681 |
| ORF Size | 1638 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016681.4 |
| RefSeq Size | 2247 bp |
| RefSeq ORF | 1641 bp |
| Locus ID | 50883 |
| UniProt ID | Q9Z265 |
| Gene Summary | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204618 | Chek2 (untagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2), (10ug) |
CNY 4,072.00 |
|
| MR208692 | Chek2 (Myc-DDK-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) |
CNY 4,064.00 |
|
| MR208692L3 | Lenti ORF clone of Chek2 (Myc-DDK-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) |
CNY 6,460.00 |
|
| MR208692L4 | Lenti ORF clone of Chek2 (mGFP-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) |
CNY 6,460.00 |
