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Fpgs (BC005484) Mouse Tagged ORF Clone
CAT＃: MG208684
- TrueORF®
Fpgs (tGFP-tagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401)
ORF Plasmid: DDK
"BC005484" in other vectors (4)
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CNY 3,420.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA408187 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208684 representing BC005484
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTATCAGGATGCTGTGCGCACGCTCAACACCCTGCAAACCAATGCCAGCTACCTGGAGCAGGTAA AGCGCCAACGGAGTGACCCCCAGGCGCAGCTGGAGGCTATGGAGATGTACCTGGCACGGAGTGGACTGCA GGTGGAGGACTTGAACCGGCTAAACATTATTCATGTCACTGGGACCAAAGGGAAGGGCTCCACCTGTGCC TTCACCGAACGGATCCTGCGGAATTACGGCCTGAAGACCGGCTTCTTTAGCTCTCCTCACATGGTGCAGG TGCGGGAGCGGATTCGAATCAACGGGAAACCAATCAGCCCCGAGCTCTTCACCAAGCACTTCTGGTGCCT CTATAACCAGCTGGAGGAGTTCAAGGACGACAGCCATGTCTCCATGCCCTCTTACTTCCGCTTCCTCACA CTCATGGCCTTCCATGTCTTCCTCCAAGAGAAGGTGGACCTGGCAGTGGTGGAGGTGGGCATTGGCGGGG CTTTTGACTGCACCAACATCATCAGAAAGCCAGTGGTGTGTGGAGTCTCCTCTCTTGGCATTGACCACAC CAGTCTACTAGGAGATACAGTGGAGAAAATAGCATGGCAGAAAGGGGGCATCTTTAAGCCTGGTGTCCCT GCCTTCACTGTGGTGCAGCCAGAAGGTCCCCTGGCTGTGCTGAGGGATCGAGCCCAGCAGATTGGATGCC CGTTGTACCTGTGTCCGCCATTGGAAGCCCTGGAGGAGGTTGGACTGCCATTGAGCCTGGGTCTGGAGGG AGCACACCAGCGGTCTAATGCTGCCTTGGCCTTGCAGCTGGCCCACTGTTGGCTGGAGCGGCAGGACCAC CAAGACATCCAGGAGCTGAAGGTATCCAGGCCAAGCATACGGTGGCAGCTGCCCCTGGCACCTGTGTTCC GCCCTACCCCTCACATGAGGCGTGGGCTTCGGGACACAGTGTGGCCTGGCCGGACACAGATACTCCAGCG GGGACCCCTTACCTGGTACCTGGATGGCGCCCATACCACCAGCAGTGTGCAGGCCTGTGTGCACTGGTAC CGCCAGTCATTGGAGCGCAGCAAACGCACCGATGGAGGGTCCGAAGTACACATCTTGCTCTTCAACTCTA CTGGTGACAGGGACTCTGCTGCCCTGCTGAAGCTGCTGCAGCCCTGCCAGTTTGACTACGCTGTCTTCTG CCCCAACGTGACAGAGGTTTCATCCATAGGAAATGCAGACCAGCAGAACTTCACTGTGACTCTGGACCAG GTGCTGCTCCGCTGCCTCCAACACCAGCAGCATTGGAACGGCCTGGCTGAGAAACAGGCTAGCTCCAACC TCTGGAGCAGCTGCGGCCCAGACCCTGCTGGGCCAGGCTCCCTGCTGCTGGCCCCGCACCCACCTCAGCC TACTAGGACGAGCTCCCTCGTTTTCAGCTGCATCTCCCACGCCTTGCTGTGGATCAGCCAAGGCCGGGAT CCCATCTTTCAGCCCCAGAGCCTTCCAAGGAATCTTCTCAACCACCCCACAGCCAACAGCGGGGCCAGCA TTCTCCGTGAGGCTGCTGCCATCCATGTACTGGTTACAGGAAGCCTGCACCTGGTGGGCGGGGTTCTGAA ACTGCTGGATCCCTCTATGTCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208684 representing BC005484
Red=Cloning site Green=Tags(s) MEYQDAVRTLNTLQTNASYLEQVKRQRSDPQAQLEAMEMYLARSGLQVEDLNRLNIIHVTGTKGKGSTCA FTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQLEEFKDDSHVSMPSYFRFLT LMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVP AFTVVQPEGPLAVLRDRAQQIGCPLYLCPPLEALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDH QDIQELKVSRPSIRWQLPLAPVFRPTPHMRRGLRDTVWPGRTQILQRGPLTWYLDGAHTTSSVQACVHWY RQSLERSKRTDGGSEVHILLFNSTGDRDSAALLKLLQPCQFDYAVFCPNVTEVSSIGNADQQNFTVTLDQ VLLRCLQHQQHWNGLAEKQASSNLWSSCGPDPAGPGSLLLAPHPPQPTRTSSLVFSCISHALLWISQGRD PIFQPQSLPRNLLNHPTANSGASILREAAAIHVLVTGSLHLVGGVLKLLDPSMSQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC005484 |
| ORF Size | 1637 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005484, AAH05484 |
| RefSeq Size | 2215 bp |
| RefSeq ORF | 1637 bp |
| Locus ID | 14287 |
| Gene Summary | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Dihydrofolate, tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate and 5-formyltetrahydrofolate are the best substrates. Folic acid and 5-methyltetrahydrofolate can also act as substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200888 | Fpgs (untagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401), (10ug) |
CNY 4,064.00 |
|
| MR208684 | Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401) |
CNY 4,056.00 |
|
| MR208684L3 | Lenti ORF clone of Fpgs (Myc-DDK-tagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401) |
CNY 5,040.00 |
|
| MR208684L4 | Lenti ORF clone of Fpgs (mGFP-tagged) - Mouse folylpolyglutamyl synthetase (cDNA clone MGC:7296 IMAGE:3485401) |
CNY 5,040.00 |
