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Treh (BC019214) Mouse Tagged ORF Clone
CAT＃: MG208643
- TrueORF®
Treh (tGFP-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708)
ORF Plasmid: DDK
"BC019214" in other vectors (4)
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CNY 5,990.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2210412M19Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208643 representing BC019214
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTGGGAGCTGCACCTGCTGCTTCTGCTGGGGCTGGGACTTAGGTCCCAGGAGGCCCTGCCACCAC CCTGTGAGAGATCTACTGCCATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGATGAC AAGCAGTTTGTGGATATGTCACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTGGCCA CAGTCCACAACCACAGCATCCCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCGTGGG GCAGGAGCTGCAGTCCTGGACCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTCGGAT GCTAATCTGCGTGTCTGGGCGGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGGGACTCGTAC TGGGTGATGGAAGGCCTGCTTCTTTCTGAGATGGCCTCAACAGTGAAGGGTATGCTGCAGAACTTTCTGG ATCTGGTGAAGACCTACGGACATATCCCCAATGGTGGACGCATATATTACCTGCAACGGAGCCAGCCCCC ACTCCTGACTCTCATGATGGATCGATATGTAGCTCATACCAAGGATGTCGCCTTCCTTCAGGAGAATATT GGGACTCTAGCCTCTGAACTGGACTTCTGGACTGTGAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAA GCTATGTCTTAAATCGCTACTATGTCCCTTATGGGGGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGA ATTGGCAAACTCTGTGCCAGAAGGGGACCGAGAGACTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCT GGCTGGGACTTCTCTTCACGCTGGCTTGTTGGAGGCCCAGACCCTGATTTGCTCAGCAGCATCCGAACCA GCAAAATGGTACCCGCTGATCTGAACGCGTTCCTGTGCCAAGCAGAGGAACTGATGAGTAACTTCTACTC CAGACTAGGGAACAACACAGAGGCCACAAAGTACAGGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAA GCTGTCCTGTGGGACGAGCAGAAGGGTGCCTGGTTCGACTATGACTTGGAAAAGGGGAAGAAGAACCTGG AGTTTTATCCCTCCAACCTCACCCCACTTTGGGCTGGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGC TCTGAAGTACTTGGAGGACAGCAAGATCTTGACCTACCAATATGGAATCCCAACCTCTCTTCGTAACACA GGCCAGCAGTGGGACTTCCCCAATGCCTGGGCCCCACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGT CAGCTTCCCCCCGGACTCAGGAGGTGGCTTTCCAGCTGGCCCAGAATTGGATCAAAACCAACTTCAAAGT CTACTCCCAAAAGTCAGCGATGTTTGAGAAGTATGACATCAGCAACGGTGGACATCCAGGTGGAGGAGGG GAGTATGAAGTTCAGGAAGGATTTGGCTGGACAAACGGATTGGCCCTGATGCTTCTGGATCGCTATGGTG ACCAGTTGACTTCAGGGACCCAGTTAGCTTCCCTGGGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAG TCTTCTGCTACAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208643 representing BC019214
Red=Cloning site Green=Tags(s) MTWELHLLLLLGLGLRSQEALPPPCERSTAMESSCTKFRWPSSTKMTSSLWICHWPHLQMKSCRSSVSWP QSTTTASPRNSFRNLSRVTSSPWGRSCSPGPLRTGRTALSSCRRSRMLICVSGRRSYTRSGKSWERRDSY WVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLTLMMDRYVAHTKDVAFLQENI GTLASELDFWTVNRTVSVVSGGQSYVLNRYYVPYGGPRPESYRKDAELANSVPEGDRETLWAELKAGAES GWDFSSRWLVGGPDPDLLSSIRTSKMVPADLNAFLCQAEELMSNFYSRLGNNTEATKYRNLRAQRLAAME AVLWDEQKGAWFDYDLEKGKKNLEFYPSNLTPLWAGCFSDPSVADKALKYLEDSKILTYQYGIPTSLRNT GQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAMFEKYDISNGGHPGGGG EYEVQEGFGWTNGLALMLLDRYGDQLTSGTQLASLGPHCLVAALLLSLLLQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC019214 |
| ORF Size | 1623 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019214.1 |
| RefSeq Size | 1972 bp |
| RefSeq ORF | 1625 bp |
| Locus ID | 58866 |
| Gene Summary | This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218346 | Treh (untagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708), (10ug) |
CNY 6,940.00 |
|
| MR208643 | Treh (Myc-DDK-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708) |
CNY 5,420.00 |
|
| MR208643L3 | Lenti ORF clone of Treh (Myc-DDK-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708) |
CNY 7,320.00 |
|
| MR208643L4 | Lenti ORF clone of Treh (mGFP-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (cDNA clone MGC:29262 IMAGE:5064708) |
CNY 7,320.00 |
