Grb10 (BC016111) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730571D09Rik; AI325020; Meg1; mKIAA0207
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208633 representing BC016111 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAACGATATTAACTCGTCCGTGGAAAGCCTTAACTCAGCTTGCAACATGCAGTCTGATACTGATA CTGCACCACTTCTTGAGGATGGCCAGCATGCCAGCAACCAGGGAGCAGCATCTAGCTCCCGGGGACAGCC ACAGGCGTCCCCGAGGCAGAAAATGCAACGCTCGCAGCCTGTGCACATTCTCAGGCGCCTTCAGGAGGAA GACCAGCAGTTAAGAACTGCATCTCTTCCGGCCATCCCCAACCCATTTCCGGAGCTCACTGGTGCGGCCC CTGGGAGCCCTCCTTCGGTTGCTCCTAGCTCCTTACCTCCTCCTCCGAGCCAGCCACCTGCCAAGCATGA TGTCAAAGTCTTTAGTGAAGATGGGACCAGCAAAGTGGTGGAGATTCTAACCGACATGACAGCCAGGGAC CTGTGCCAGCTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACTCTGGTGGAACACCACC CACAACTGGGATTAGAGAGGTGCCTGGAGGACCATGAGATCGTGGTCCAAGTGGAGAGTACCATGCCAAG TGAGAGCAAATTCTTATTCAGAAAGAATTATGCGAAGTACGAGTTCTTTAAGAATCCAGTGAACTTCTTC CCGGATCAGATGGTCAATTGGTGCCAGCAGTCCAACGGTGGCCAGGCGCAGCTTCTGCAGAATTTTCTGA ACACCAGCAGCTGCCCTGAGATCCAGGGGTTCTTGCAGGTGAAAGAGGTAGGACGCAAGTCTTGGAAGAA GCTGTATGTGTGCCTGCGCAGATCTGGCCTCTATTACTCCACCAAGGGGACTTCAAAAGAACCCAGACAC CTGCAGCTGCTGGCTGACCTGGAAGAAAGCAGCATCTTCTACCTGATTGCTGGAAAGAAGCAGTACAACG CGCCGAATGAACATGGGATGTGCATCAAGCCAAACAAAGCGAAGACCGAGATGAAGGAGCTTCGTCTGCT CTGTGCCGAAGATGAGCAGATCCGTACTTGCTGGATGACTGCCTTCAGACTGCTCAAGTACGGAATGCTC CTGTACCAAAACTATCGCATCCCACAGAGGAAGGGTCTGCCCCCTCCTTTCAACGCACCTATGCGCAGTG TTTCTGAGAATTCTCTTGTGGCCATGGATTTTTCTGGACAAATCGGAAGAGTGATCGATAACCCGGCTGA AGCCCAGAGTGCTGCCCTGGAAGAGGGCCATGCCTGGCGTAAGCGGAGCACACGGATGAATATCCTAAGC AGCCAAAGCCCACTGCATCCTTCTACCCTGAATGCAGTGATTCACAGGACTCAGCATTGGTTCCATGGAC GTATCTCCCGCGAGGAGTCTCACAGGATCATCAAGCAACAAGGTCTCGTGGACGGGCTGTTCCTCCTTCG TGACAGCCAGAGTAATCCAAAGGCGTTCGTACTGACACTGTGCCATCACCAGAAGATTAAAAACTTCCAG ATCTTACCTTGCGAGGATGATGGGCAGACCTTCTTCACTCTGGATGATGGGAACACCAAGTTCTCCGATC TGATCCAGCTGGTCGACTTCTACCAGCTCAACAAAGGTGTTCTGCCCTGCAAGCTGAAACACCACTGCAT CCGCGTGGCCTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208633 representing BC016111 Red=Cloning site Green=Tags(s) MNNDINSSVESLNSACNMQSDTDTAPLLEDGQHASNQGAASSSRGQPQASPRQKMQRSQPVHILRRLQEE DQQLRTASLPAIPNPFPELTGAAPGSPPSVAPSSLPPPPSQPPAKHDVKVFSEDGTSKVVEILTDMTARD LCQLLVYKSHCVDDNSWTLVEHHPQLGLERCLEDHEIVVQVESTMPSESKFLFRKNYAKYEFFKNPVNFF PDQMVNWCQQSNGGQAQLLQNFLNTSSCPEIQGFLQVKEVGRKSWKKLYVCLRRSGLYYSTKGTSKEPRH LQLLADLEESSIFYLIAGKKQYNAPNEHGMCIKPNKAKTEMKELRLLCAEDEQIRTCWMTAFRLLKYGML LYQNYRIPQRKGLPPPFNAPMRSVSENSLVAMDFSGQIGRVIDNPAEAQSAALEEGHAWRKRSTRMNILS SQSPLHPSTLNAVIHRTQHWFHGRISREESHRIIKQQGLVDGLFLLRDSQSNPKAFVLTLCHHQKIKNFQ ILPCEDDGQTFFTLDDGNTKFSDLIQLVDFYQLNKGVLPCKLKHHCIRVAL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016111
ORF Size	1625 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016111, AAH16111
RefSeq Size	4799 bp
RefSeq ORF	1625 bp
Locus ID	14783
Gene Summary	Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. A similar role in the mediation of ubiquitination has also been suggested with INSR. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.[UniProtKB/Swiss-Prot Function]

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