Gnl3 (BC037996) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Ns, MGC46970
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208603 representing BC037996 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGCCTAAGTTAAAGAAAGCGAGTAAACGTATGACCTGCCATAAGCGATATAAAATTCAAAAAA AGGTCCGAGAACATCATCGAAAATTAAGGAAGGAAGCTAAAAAACGGGGTCACAAGAAGCCTAGGAAGGA CCCAGGTGTTCCAAATAGTGCTCCCTTTAAAGAGGCTCTTCTTCGTGAAGCTGAACTAAGGAAACAGCAG CTTGAAGAACTAAAACAGCAGCAGAAACTTGATAGGCAAAAAGAGCAAGAAAGGAAAAGAAAACTTGAAG TTAGCCCTGGTGATGAGCAGTCTAATGTGGAAACTAGGGAGGAATCTGACGAGCCCAAAAGAAAGAAAGC CAAAGCAGGCAAACAGAATCCAAAGAAGTTACATTGCCAGGAACTTAAAAAGGTGATTGAAGCCTCAGAC ATTGTGTTAGAAGTTTTGGATGCCAGAGATCCTCTTGGTTGCAGGTGTCCTCAGATAGAAGAAGCTGTTA TCCAGAGTGGGAGTAAGAAGCTGATACTTGTATTAAATAAGTCTGATCTAGTACCAAAGGAGAATTTGGA GAACTGGCTAAATTATTTGAATAAAGAATTGCCAACCGTGGTGTTCAAAGCCTCAACAAACTTAAAGAAC AGAAAGACATTCAAGATAAAAAAGAAGAAAGTTGTTCCATTCCAAAGCAAAATCTGCTGTGGCAAGGAAG CCCTTTGGAAGCTTCTTGGAGATTTTCAGCAGTCCTGTGGAAAGGATATTCAAGTTGGAGTGATTGGTTT CCCAAATGTGGGGAAAAGCAGTGTCATTAATAGCTTAAAACAAGAATGGATTTGCAATGTTGGGATTTCC ATGGGACTTACAAGGAGCATGCAGATTGTCCCCTTAGACAAGCAGATCACAATCATAGACAGTCCATGCC TAATTATCTCACCTTGTAACTCCCCCACTGCACTTGCCCTTCGGAGTCCAGCAAGCATTGAGGAACTAAG ACCGCTGGAGGCTGCCAGTGCCATTCTGTCTCAGGCTGATAATGAACAGGTGGTGTTAAAATATACTGTC CCTGAGTATAAGGATTCTCTGCATTTTTTTACTAAACTTGCTCAAAGAAGAGGTCTGCACCAAAAAGGTG GAAGCCCAAATGTGGAAAGTGCTGCTAAGCTGGTGTGGTCTGAGTGGACAGGTGCCTCATTAGGTTACTA TTGCCATCCCCCTGCATCCTGGAATCATTCTCTGCATTTTAATGAGAATATTGCAGCAGTCATGAAGAAG GGCTTTAATCTAGAAGAACTAGAAAAGAATAATGCACACAGCATACAAGTCCTCAAGGGCCCTCATTTAA CTAATAGAATCCTTTTTCGGTCTTCGGGCCTGACAAATGGAATACTAGACGAGAAGGACATAGTCGAAGA ATCACCAAGACAGACAGAAGACAAACAGGATGCTGACGATCAAGAAAATGGTAGTGGTGAAAGAAATGCA GAGATCTCAGATGTGGCCCCTGTAGAAGAGACCAGGGAGCTGTCACCTGAGCAATCAACAGCAGGTAAGC CATCTGACGGGTCGTCTGCCTTGGATAGAGCGAGTCAAGAGGATGAAACCTATGACTTCACCACAGATTA TATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208603 representing BC037996 Red=Cloning site Green=Tags(s) MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQQ LEELKQQQKLDRQKEQERKRKLEVSPGDEQSNVETREESDEPKRKKAKAGKQNPKKLHCQELKKVIEASD IVLEVLDARDPLGCRCPQIEEAVIQSGSKKLILVLNKSDLVPKENLENWLNYLNKELPTVVFKASTNLKN RKTFKIKKKKVVPFQSKICCGKEALWKLLGDFQQSCGKDIQVGVIGFPNVGKSSVINSLKQEWICNVGIS MGLTRSMQIVPLDKQITIIDSPCLIISPCNSPTALALRSPASIEELRPLEAASAILSQADNEQVVLKYTV PEYKDSLHFFTKLAQRRGLHQKGGSPNVESAAKLVWSEWTGASLGYYCHPPASWNHSLHFNENIAAVMKK GFNLEELEKNNAHSIQVLKGPHLTNRILFRSSGLTNGILDEKDIVEESPRQTEDKQDADDQENGSGERNA EISDVAPVEETRELSPEQSTAGKPSDGSSALDRASQEDETYDFTTDYI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037996
ORF Size	1616 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC037996, AAH37996
RefSeq Size	1806 bp
RefSeq ORF	1616 bp
Locus ID	30877
Gene Summary	May be required to maintain the proliferative capacity of stem cells (By similarity). Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.