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Paf1 (NM_019458) Mouse Tagged ORF Clone
CAT＃: MG208572
- TrueORF®
Paf1 (tGFP-tagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S
ORF Plasmid: DDK
"NM_019458" in other vectors (4)
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CNY 5,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730511K23Rik; AA675045 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208572 representing NM_019458
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCACCATCCAGACCCAGGCCCAGAGGGAAGATGGCCACAGGCCCAATTCCCACCGGACCTTGC CTGAGAGGTCTGGAGTAGTCTGCCGAGTCAAGTACTGCAATAGCCTTCCTGACATCCCATTTGACCCCAA GTTCATCACCTACCCCTTCGACCAGAACAGGTTTGTTCAGTACAAAGCAACCTCCTTGGAGAAACAGCAC AAACATGACCTCCTGACTGAGCCAGATCTGGGGGTCACCATTGATCTCATCAACCCTGACACCTACCGCA TTGATCCCAATGTTCTTCTGGATCCGGCTGATGAGAAGCTTCTAGAAGAGGAGATTCAGGCTCCCACCAG CTCTAAGAGATCCCAGCAGCATGCAAAGGTGGTACCGTGGATGCGTAAGACAGAGTATATCTCCACTGAG TTCAACAGATATGGCATCTCCAATGAGAAGCCTGAAGTCAAGATTGGGGTTTCTGTGAAACAGCAGTTCA CAGAGGAAGAAATATACAAAGACAGGGACAGCCAAATCACAGCCATTGAAAAGACTTTTGAAGATGCCCA GAAATCGATCTCCCAGCATTACAGCAAGCCCCGAGTGACACCAGTGGAGGTCATGCCTGTCTTCCCAGAT TTTAAGATGTGGATAAACCCATGTGCTCAGGTCATTTTTGACTCAGACCCAGCCCCCAAGGACACAAGTG GTGCAGCTGCATTAGAGATGATGTCTCAGGCCATGATCAGGGGCATGATGGATGAGGAAGGGAACCAGTT TGTGGCTTATTTTCTGCCTGTGGAAGAAACACTAAAGAAACGAAAGCGGGACCAAGAGGAAGAGATGGAC TATGCACCAGATGATGTGTATGACTATAAGATTGCTCGGGAATACAACTGGAATGTGAAGAACAAGGCCA GTAAGGGCTATGAGGAGAACTATTTCTTCATCTTCAGAGAGGGTGACGGAGTTTACTACAATGAGCTGGA GACCAGGGTCCGTCTAAGTAAGCGCCGGGCCAAGGCTGGGGTTCAGTCAGGTACCAATGCCTTGCTTGTC GTCAAACACCGTGACATGAATGAGAAGGAATTAGAAGCCCAGGAGGCACGAAAGGCCCAGTTGGAAAACC ACGAACCAGAGGAAGAGGAGGAGGAAGAGATGGAGGCTGAAGAGAAAGAAGCTGGGGGCTCAGATGAGGA ACAGGAGAAGGGTAGCAGCAGTGAGAAAGAAGGCAGCGAGGATGAACACTCTGGAAGTGAGAGTGACCGA GAAGAGGGTGACAGAGATGAGGCAAGTGACAAGAGTGGCAGCGGTGAGGATGAAAGCAGCGAGGATGAAG CACGTGCTGCCCGGGACAAAGAGGAGATTTTTGGTAGTGATGCTGATTCAGAAGATGATGCTGACTCTGA TGATGAGGACAGAGGGCAGGCCCACAGGGGCAGTGACAATGACTCGGACAGTGGCAGTGATGGTGGTGGC CAGAGGAGCCGCAGCCAGAGCAGGAGTCGTAGCCGGAGTGCCAGCCCCTTCCCCAGTGGCAGTGAGCACT CAGCTCAGGAAGATGGCAGTGAAGCAGCAGCCTCTGATTCCAGTGAGGCTGACAGTGACAGTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208572 representing NM_019458
Red=Cloning site Green=Tags(s) MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQH KHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTE FNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPD FKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMD YAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLV VKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMEAEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESDR EEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAHRGSDNDSDSGSDGGG QRSRSQSRSRSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019458 |
| ORF Size | 1605 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019458.2 |
| RefSeq Size | 1953 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 54624 |
| UniProt ID | Q8K2T8 |
| Gene Summary | Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203167 | Paf1 (untagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1), (10ug) |
CNY 3,992.00 |
|
| MR208572 | Paf1 (Myc-DDK-tagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1) |
CNY 3,992.00 |
|
| MR208572L3 | Lenti ORF clone of Paf1 (Myc-DDK-tagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1) |
CNY 6,560.00 |
|
| MR208572L4 | Lenti ORF clone of Paf1 (mGFP-tagged) - Mouse Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (Paf1) |
CNY 6,560.00 |
