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Cmah (BC055079) Mouse Tagged ORF Clone
CAT＃: MG208522
- TrueORF®
Cmah (tGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (cDNA clone MGC:60630 IMAGE:30077348), complete
ORF Plasmid: DDK
"BC055079" in other vectors (4)
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CNY 3,330.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208522 representing BC055079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGACAGGAAACAGACAGCTGAGACCCTGCTGACCCTGTCTCCTGCTGAAGTTGCCAACCTCAAGG AAGGGATCAATTTTTTTCGAAATAAGACTACTGGGAAAGAGTACATTTTATACAAGGAGAAGGACCATCT AAAGGCATGCAAGAACCTCTGCAAGCACCAGGGAGGCCTGTTCATGAAAGACATCGAGGATTTAGATGGA AGGTCCGTTAAATGCACAAAGCACAACTGGAAGTTAGACGTGAGCACCATGAAATATATCAACCCTCCAG GGAGCTTCTGTCAAGACGAGCTCGTTATTGAAATGGATGAAAACAATGGGCTTTCCCTGGTAGAACTGAA CCCTCCTAACCCCTGGGACTCTGATCCCAGGTCTCCTGAAGAATTAGCTTTTGGGGAAGTACAGATAACA TATCTCACTCATGCCTGCATGGACCTCAAGTTGGGAGACAAGCGAATGGTATTTGACCCTTGGTTAATTG GCCCTGCTTTTGCCCGAGGATGGTGGTTGCTACATGAGCCTCCATCTGACTGGTTGGAGAGGCTGTGCAA AGCAGACCTCATTTATATCAGCCACATGCACTCAGACCACCTGAGCTACCCTACCCTGAAGCAGCTTTCC CAGAGACGACCAGACATTCCCATTTATGTTGGCGACACAGAAAGGCCTGTGTTTTGGAACCTGGATCAGA GTGGCGTCGGGTTAACTAACATCAACGTGGTTCCATTTGGAATATGGCAACAGGTAGACAAAAGTCTGCG GTTCATGATCTTGATGGACGGCGTTCATCCTGAGATGGACACATGCATTATCGTGGAGTACAAAGAGGAA TGGAAAGCCCAGTTCATTAAGGCTGAAAGAAGAAAGCTTCTGAATTACAAAGCTCAGCTGGTGAAGGACC TGCAGCCCCGAATCTACTGTCCGTTTGCTGGGTACTTTGTGGAGTCTCATCCATCTGACAAGTACATTAA GGAAACAAACACCAAAAATGACCCAAATCAGCTCAACAATCTTATCAGGAAAAACTCTGACGTGGTGACA TGGACCCCACGACCTGGCGCTGTCCTCGACCTTGGCAGGATGCTGAAGGACCCAACAGACAGCAAGGGCA TTGTGGAGCCTCCAGAGGGGACAAAGATTTACAAGGATTCCTGGGACTTTGGCCCGTACCTGGAGATCTT GAATTCTGCTGTCAGAGATGAAATCTTCTGTCATTCATCCTGGATTAAAGAGTACTTCACGTGGGCTGGA TTTAAGAATTACAACCTGGTGGTCAGGATGATTGAAACAGATGAAGATTTCAGCCCTTTTCCTGGAGGGT ACGACTATCTGGTGGACTTTCTAGATTTATCCTTTCCGAAAGAAAGACCCAGCCGGGAGCATCCTTATGA AGAAACCCATAGCCGGGTGGATGTCATCAGGTACGTGGTGAAGAACGGCCTGCTGTGGGATGATCTGTAT ATTGGATTCCAGACCCGATTGCTGCGGGACCCTGATATATACCATCATCTGTTTTGGAACCATTTTCAGA TAAAACTCCCTCTAACACCACCCAACTGGAAGTCGTTCCTAATGCACTGTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208522 representing BC055079
Red=Cloning site Green=Tags(s) MMDRKQTAETLLTLSPAEVANLKEGINFFRNKTTGKEYILYKEKDHLKACKNLCKHQGGLFMKDIEDLDG RSVKCTKHNWKLDVSTMKYINPPGSFCQDELVIEMDENNGLSLVELNPPNPWDSDPRSPEELAFGEVQIT YLTHACMDLKLGDKRMVFDPWLIGPAFARGWWLLHEPPSDWLERLCKADLIYISHMHSDHLSYPTLKQLS QRRPDIPIYVGDTERPVFWNLDQSGVGLTNINVVPFGIWQQVDKSLRFMILMDGVHPEMDTCIIVEYKEE WKAQFIKAERRKLLNYKAQLVKDLQPRIYCPFAGYFVESHPSDKYIKETNTKNDPNQLNNLIRKNSDVVT WTPRPGAVLDLGRMLKDPTDSKGIVEPPEGTKIYKDSWDFGPYLEILNSAVRDEIFCHSSWIKEYFTWAG FKNYNLVVRMIETDEDFSPFPGGYDYLVDFLDLSFPKERPSREHPYEETHSRVDVIRYVVKNGLLWDDLY IGFQTRLLRDPDIYHHLFWNHFQIKLPLTPPNWKSFLMHCD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC055079 |
| ORF Size | 1595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055079, AAH55079 |
| RefSeq Size | 2995 bp |
| RefSeq ORF | 1595 bp |
| Locus ID | 12763 |
| Gene Summary | Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217693 | Cmah (untagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (cDNA clone MGC:60630 IMAGE:30077348), complete, (10ug) |
CNY 6,940.00 |
|
| MR208522 | Cmah (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (cDNA clone MGC:60630 IMAGE:30077348), complete |
CNY 3,952.00 |
|
| MR208522L3 | Lenti ORF clone of Cmah (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (cDNA clone MGC:60630 IMAGE:30077348), complete |
CNY 6,352.00 |
|
| MR208522L4 | Lenti ORF clone of Cmah (mGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid hydroxylase (cDNA clone MGC:60630 IMAGE:30077348), complete |
CNY 4,940.00 |
