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Supt20 (NM_019995) Mouse Tagged ORF Clone
CAT＃: MG208520
- TrueORF®
Supt20 (tGFP-tagged) - Mouse DNA segment, Chr 3, ERATO Doi 300, expressed (D3Ertd300e)
ORF Plasmid: DDK
"NM_019995" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA667204; AI450544; D3Ertd300e; Fam48a; p38IP; Supt20h |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208520 representing NM_019995
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACAAGCTGTAGAACAAGCTTTGGATTGCGCAGAGTACATCGTGGAAAGTGCTCAGCAGAGACCTC CTAAAAGGAAATACCTGTCTAGTGGAAGAAAGTCTATATTTCAAAAACTTTATGACTTATATGTTGAAGA ATGTGAAAAAGAACCCGAGGTTAAGAAATTAAGAAGAAATGTGAACTTGTTAGAGAAGCTTGTTATGCAG GAGACTTTGTCGTGTTTAGTAGTCAATCTGTACCCAGGAAATGAAGGCTATTCTCTGATGCTCAGGGGGA AAAATGGATCAGATTCTGAGACCATCCGATTGCCTTATGAAGAAGGAGAATTGCTTGAATACTTAGATGC AGAAGAATTACCTCCTATTTTGGTGGATCTCCTTGAAAAATCACAGGTTAATATTTTTCATTGTGGATGT GTGATAGCAGAAATACGTGACTATAGGCAGTCCAGTAATATGAAGTCTCCTGGTTACCAAAGTAGGCACA TTCTCTTACGTCCAACAATGCAGACTTTAGTCTGTGATGTCCATTCTATAACAAGTGACAACCATAAGTG GACCCAGGAAGACAAACTTTTGCTTGAGAGCCAGCTGATTCTAGCTACAGCAGAGCCACTGTGTCTTGAC CCATCGGTGGCAGTAGCGTGCACTGCCAATAGGCTGCTCTATAACCGGCAGAAGATGAACACTCGCCCAA TGAAACGGTGTTGGAAGAGGTACTCCAGGTCCTCCCTGAACCGCCAGCAGGACCTCTCTCACGGCCCTCC TCCCCCTCAGCTGAGACTACTTGATTTCTTACAAAAGCGGAAGGAAAGGAAAGCAGGCCAGCATTATGAC CTCAAAATTTCCAAAGCAGGAAATTGTGTAGACATGTGGAAACGGAGCCCCTGTAACTTGGCTGTGCCTT CTGAAGTAGATGTGGAAAAATATGCTAAAGTGGAGGAATCCATCAAATCTGACGACTCACAGCCAACAAT GTGGCCAGCTCATGATGTAAAAGACGATTATGTATTTGAATGTGAAGGTGGTAATCAGTATCAGAAAACA AAGCTGACCATCTTGCAGTCTCTTGGGGATCCACTTTACTATGGTAAAATACAGCCGTGGAAAGCAGATG AAGAAAATGACAGCCAAATGTCTCCATCCCACTCCTCTGCTGATGACCATTCAAACTGGTTTGTCATTGG GTCAAAGACTGACGCAGAGAGGGTAGTTAATCAATACCAGGAGTTGGTCCAGAATGAAGCCAAATGCCCA GTGAAGATGTCACACAGCAGTAGTGGTTCAGCCGCTCTCAGTCCAGGGGAGGAGGCAGAACAGGCGGAGA CCTCGTCCATTCAGTCTTCAGTTTTGGGAAAGGGAGTAAAACACCGACCCCCACCCATCAAACTTCCCTC AGGCTCAGGAAATAGTTCCTCAGGTAACTATTTCACAGCACAGCAGGCCAGCAGCTTCCTTAAATCTCCA ACTCCTCCTCCATCGTGTAAGCCAAGCCTTTCTCGGAAGTCTTCTGTGGACCTCAGTCAAGTTAGCATGC TTTCTCCTGCCGCCCTGTCCCCTGCCAGCTCATCCCAGAGACATGAAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208520 representing NM_019995
Red=Cloning site Green=Tags(s) MQQAVEQALDCAEYIVESAQQRPPKRKYLSSGRKSIFQKLYDLYVEECEKEPEVKKLRRNVNLLEKLVMQ ETLSCLVVNLYPGNEGYSLMLRGKNGSDSETIRLPYEEGELLEYLDAEELPPILVDLLEKSQVNIFHCGC VIAEIRDYRQSSNMKSPGYQSRHILLRPTMQTLVCDVHSITSDNHKWTQEDKLLLESQLILATAEPLCLD PSVAVACTANRLLYNRQKMNTRPMKRCWKRYSRSSLNRQQDLSHGPPPPQLRLLDFLQKRKERKAGQHYD LKISKAGNCVDMWKRSPCNLAVPSEVDVEKYAKVEESIKSDDSQPTMWPAHDVKDDYVFECEGGNQYQKT KLTILQSLGDPLYYGKIQPWKADEENDSQMSPSHSSADDHSNWFVIGSKTDAERVVNQYQELVQNEAKCP VKMSHSSSGSAALSPGEEAEQAETSSIQSSVLGKGVKHRPPPIKLPSGSGNSSSGNYFTAQQASSFLKSP TPPPSCKPSLSRKSSVDLSQVSMLSPAALSPASSSQRHES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019995 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019995.1, NP_064379.1 |
| RefSeq Size | 1972 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 56790 |
| UniProt ID | Q7TT00 |
| Gene Summary | Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation-induced ATG9A trafficking during autophagy.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217594 | Supt20 (untagged) - Mouse family with sequence similarity 48, member A (Fam48a), (10ug) |
CNY 6,840.00 |
|
| MR208520 | Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) |
CNY 3,952.00 |
|
| MR208520L3 | Lenti ORF clone of Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) |
CNY 6,460.00 |
|
| MR208520L4 | Lenti ORF clone of Supt20 (mGFP-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) |
CNY 6,460.00 |
