Txnrd2 (NM_013711) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Untagged ORF Clone
Synonyms	AA118373; ESTM57301; ESTM573010; TG; TGR; TR; Tr3; Trxr2; Trxrd2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208458 representing NM_013711 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGATGGTGGCGGCGATGGTGGCGGCGCTGCGTGGACCCAGCAGGCGCTTCCGGCCGCGGACAC GGGCTCTGACACGCGGGACAAGGGGCGCGGCGAGTGCAGCGGGAGGGCAGCAGAGCTTTGATCTCTTGGT GATCGGTGGGGGATCCGGTGGCCTAGCTTGTGCCAAGGAAGCTGCTCAGCTGGGAAAGAAGGTGGCTGTG GCTGACTATGTGGAACCTTCTCCCCGAGGCACCAAGTGGGGCCTTGGTGGCACCTGTGTCAACGTGGGTT GCATACCCAAGAAGCTGATGCATCAGGCTGCACTGCTGGGGGGCATGATCAGAGATGCTCACCACTATGG CTGGGAGGTGGCCCAGCCTGTCCAACACAACTGGAAGACAATGGCAGAAGCCGTGCAAAACCATGTGAAA TCCTTGAACTGGGGTCATCGCGTCCAACTGCAGGACAGGAAAGTCAAGTACTTTAACATCAAAGCCAGCT TTGTGGATGAGCACACAGTTCGCGGTGTGGACAAAGGCGGGAAGGCGACTCTGCTTTCAGCTGAGCACAT TGTCATTGCTACAGGAGGACGGCCAAGGTACCCCACACAAGTCAAAGGAGCCCTGGAATATGGAATCACA AGTGACGACATCTTCTGGCTGAAGGAGTCCCCTGGGAAAACGTTGGTGGTTGGAGCCAGCTATGTGGCCC TAGAGTGTGCTGGCTTCCTCACTGGAATTGGACTGGATACCACTGTCATGATGCGCAGCATCCCTCTCCG AGGCTTTGACCAGCAAATGTCATCTTTGGTCACAGAGCACATGGAGTCTCATGGCACCCAGTTCCTGAAA GGCTGTGTCCCCTCCCACATCAAAAAACTCCCAACTAACCAGCTGCAGGTCACTTGGGAGGATCATGCTT CTGGCAAGGAAGACACAGGCACCTTTGACACTGTCCTGTGGGCCATAGGGCGAGTTCCAGAAACCAGGAC TTTGAATCTGGAGAAGGCTGGCATCAGTACCAACCCTAAGAATCAGAAGATTATTGTGGATGCCCAGGAG GCTACCTCTGTTCCCCACATCTATGCCATTGGAGATGTTGCTGAGGGGCGGCCTGAGCTGACGCCCACAG CTATCAAGGCAGGAAAGCTTCTGGCTCAGCGGCTCTTTGGGAAATCCTCAACCTTAATGGATTACAGCAA TGTTCCCACAACTGTCTTTACACCACTGGAGTATGGCTGTGTGGGGCTGTCTGAGGAGGAGGCTGTGGCT CTCCATGGCCAGGAGCATGTAGAGGTTTACCATGCATATTATAAGCCCCTAGAGTTCACGGTGGCGGATA GGGATGCATCACAGTGCTACATAAAGATGGTATGCATGAGGGAGCCCCCACAACTGGTGCTGGGCCTGCA CTTCCTTGGCCCCAACGCTGGAGAAGTCACCCAAGGATTTGCTCTTGGGATCAAGTGTGGGGCTTCATAT GCACAGGTGATGCAGACAGTAGGGATCCATCCCACCTGCTCTGAGGAGGTGGTCAAGCTGCACATCTCCA AGCGCTCCGGCCTGGAGCCTACTGTGACTGGTTGCTGAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208458 representing NM_013711 Red=Cloning site Green=Tags(s) MAAMVAAMVAALRGPSRRFRPRTRALTRGTRGAASAAGGQQSFDLLVIGGGSGGLACAKEAAQLGKKVAV ADYVEPSPRGTKWGLGGTCVNVGCIPKKLMHQAALLGGMIRDAHHYGWEVAQPVQHNWKTMAEAVQNHVK SLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKGGKATLLSAEHIVIATGGRPRYPTQVKGALEYGIT SDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHMESHGTQFLK GCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQE ATSVPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTLMDYSNVPTTVFTPLEYGCVGLSEEEAVA LHGQEHVEVYHAYYKPLEFTVADRDASQCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASY AQVMQTVGIHPTCSEEVVKLHISKRSGLEPTVTGC*G TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013711
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013711.3
RefSeq Size	1902 bp
RefSeq ORF	1584 bp
Locus ID	26462
UniProt ID	Q9JLT4
Gene Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2017]

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