Rps6kb1 (BC038491) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	S6K1, p70s6k, p70/85s6k, 70kDa
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208420 representing BC038491 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGACGACGGAGGCGGGACGGCTTTTACCTAGCGCCTGACTTCCGACACAGGGAAGCTGAGGACA TGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAAGATGCAGGCTCTGAGGATGAGCTGGAGGAGGG GGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCATGGAACATTGTGAG AAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTAC TTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATACTGG GAAGATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTGAGGAATGCTAAGGACACGGCCCACACG AAAGCAGAGCGGAACATTCTGGAGGAAGTGAAACACCCTTTCATTGTGGACCTGATTTATGCCTTTCAGA CCGGAGGGAAGCTCTACCTCATCCTCGAGTATCTCAGTGGAGGAGAACTATTTATGCAGTTAGAAAGAGA GGGAATATTCATGGAAGACACAGCGTGCTTTTACTTGGCTGAAATCTCCATGGCTTTGGGGCATTTACAT CAAAAAGGGATCATCTACAGAGACCTGAAGCCGGAGAACATCATGCTTAATCACCAAGGTCACGTGAAAC TAACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACAGTCACGCACACATTTTGTGGAACAAT AGAATACATGGCCCCTGAAATCTTAATGAGAAGCGGCCACAACCGTGCTGTGGATTGGTGGAGTTTGGGA GCATTAATGTATGACATGCTGACTGGAGCACCTCCATTCACTGGGGAGAATAGAAAGAAAACAATTGACA AAATCCTCAAATGTAAACTTAATTTGCCTCCCTACCTCACACAAGAAGCTCGAGATCTGCTTAAAAAGCT GCTGAAAAGAAATGCTGCTTCTCGTCTTGGAGCTGGCCCTGGGGATGCTGGAGAAGTCCAAGCTCATCCT TTTTTCAGACACATTAACTGGGAAGAACTTCTGGCTCGGAAGGTAGAACCTCCCTTTAAGCCTCTGTTGC AATCTGAAGAGGATGTGAGTCAGTTTGATTCAAAGTTTACACGTCAGACACCTGTTGACAGCCCTGATGA CTCCACTCTCAGTGAAAGTGCCAACCAGGTCTTTCTGGGTTTTACATATGTGGCTCCATCTGTACTTGAA AGTGTGAAAGAAAAGTTTTCATTTGAACCAAAAATCCGATCTCCTAGAAGATTTATTGGTAGTCCACGAA CACCTGTCAGCCCAGTCAAATTCTCTCCTGGGGATTTCTGGGGACGAGGTGCTTCAGCCAGCACGGCAAA TCCTCAGACCCCTGTGGAATACCCAATGGAAACAAGTGGGATAGAGCAGATGGATGTGACAGTGAGCGGG GAAGCATCAGCGCCACTTCCAATCCGACAACCCAACTCCGGGCCGTACAAAAAACAAGCTTTTCCTATGA TCTCCAAACGACCAGAGCACCTGCGGATGAATCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208420 representing BC038491 Red=Cloning site Green=Tags(s) MRRRRRRDGFYLAPDFRHREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCE KFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHT KAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLH QKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLG ALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHP FFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLE SVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTVSG EASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC038491
ORF Size	1575 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC038491, AAH38491
RefSeq Size	1846 bp
RefSeq ORF	1577 bp
Locus ID	72508
Gene Summary	Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (By similarity) (PubMed:11493700, PubMed:11500364, PubMed:15060135, PubMed:18952604). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239).[UniProtKB/Swiss-Prot Function]

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