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Slc1a1 (NM_009199) Mouse Tagged ORF Clone
CAT＃: MG208367
- TrueORF®
Slc1a1 (tGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1)
ORF Plasmid: DDK
"NM_009199" in other vectors (4)
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CNY 5,496.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D130048G10Rik; EAAC1; EAAC2; EAAT3; MEAAC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208367 representing NM_009199
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCCCACGAGCTCGGGATGTGACTGGCGCCGCTTCCTACGGAATCACTGGCTGCTGCTCTCCA CCGTGGCCGCCGTGGTACTAGGAATTGTCTTAGGAGTCGTGGTTCGAGGACACAGTGAGCTCTCAAATCT GGATAAATTCTACTTTGCTTTTCCTGGGGAAATTCTGATGAGGATGCTGAAGCTGGTCATTTTGCCGCTG ATCGTATCCAGCATGATCACAGGTGTCGCTGCACTGGATTCCAATGTGTCTGGGAAGATTGGTCTGCGCG CTGTAGTATATTATTTCTCCACCACCGTCATTGCTGTAATCCTAGGTATTGTGTTAGTTGTGAGTATCAA GCCTGGTGTCACTCAGAAAGTGAATGACATCAACAGGACGGGTAAAACCCCTGAAGTCAGCACCATGGAT GCCATGTTGGACCTGATCAGGAACATGTTCCCTGAGAATCTGGTCCAAGCCTGTTTTCAGCAGTACAAAA CCAAGCGGGAAGAGGTGAAGCCTGTGGGCGATCCTGGGGGGAACGCAACGGAGGTGTCTGTCACCACAGC CATGACAACAATGTCTGAGAACAAGACAAAGGAATACAAGATCGTGGGCCTGTACTCAGACGGCATCAAT GTCCTGGGCTTGATTATCTTCTGCCTCGTCTTTGGCCTTGTCATTGGGAAAATGGGAGAAAAGGGGCAGA TTCTGGTGGACTTCTTCAATGCCTTGAGTGACGCCACCATGAAAATCGTCCAGATCATCATGTGCTACAT GCCGATTGGCATTTTGTTCCTAATTGCTGGGAAGATCATAGAAGTTGAAGACTGGGAAATATTCCGCAAG CTGGGCCTTTACATGGCCACTGTCCTGAGCGGGCTTGCAATCCACTCCCTCATAGTTCTGCCCCTGCTCT ATTTCATAGTTGTGCGGAAGAACCCTTTCCGCTTTGCCTTGGGTATGGCGCAGGCTCTCCTGACAGCTCT CATGATCTCGTCCAGTTCGGCAACCCTGCCAGTTACATTCCGCTGTGCGGAAGAAAAGAACCAGGTAGAC AAGAGGATCACGAGATTTGTGCTGCCTGTTGGTGCCACCATCAACATGGACGGCACTGCGCTCTACGAAG CTGTGGCAGCCGTGTTTATTGCGCAACTGAATGGCTTGGACCTAAGCATTGGGCAGATCGTCACCATCAG CATTACAGCCACCGCTGCCAGCATTGGAGCTGCTGGGGTGCCCCAGGCTGGCCTGGTGACCATGGTGATC GTGCTGAGTGCTGTGGGGCTGCCTGCCGAGGACGTCACCCTGATCATTGCTGTTGACTGGCTCCTGGACC GGTTCAGGACCATGGTGAACGTCCTGGGTGATGCGTTTGGGACGGGCATCGTAGAGAAGCTCTCGAAGAA GGAGCTGGAGCAGATGGATGTTTCGTCTGAAGTCAACATCGTGAACCCCTTTGCCCTGGAACCCACAACC CTCGATAACGAAGACTCAGATACCAAGAAGTCTTATGTCAATGGGGGCTTCGCGGTAGACAAATCTGACA CCATCTCGTTCACTCAGACCTCACAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208367 representing NM_009199
Red=Cloning site Green=Tags(s) MGKPTSSGCDWRRFLRNHWLLLSTVAAVVLGIVLGVVVRGHSELSNLDKFYFAFPGEILMRMLKLVILPL IVSSMITGVAALDSNVSGKIGLRAVVYYFSTTVIAVILGIVLVVSIKPGVTQKVNDINRTGKTPEVSTMD AMLDLIRNMFPENLVQACFQQYKTKREEVKPVGDPGGNATEVSVTTAMTTMSENKTKEYKIVGLYSDGIN VLGLIIFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPIGILFLIAGKIIEVEDWEIFRK LGLYMATVLSGLAIHSLIVLPLLYFIVVRKNPFRFALGMAQALLTALMISSSSATLPVTFRCAEEKNQVD KRITRFVLPVGATINMDGTALYEAVAAVFIAQLNGLDLSIGQIVTISITATAASIGAAGVPQAGLVTMVI VLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALEPTT LDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009199 |
| ORF Size | 1569 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009199.2, NP_033225.1 |
| RefSeq Size | 3708 bp |
| RefSeq ORF | 1572 bp |
| Locus ID | 20510 |
| UniProt ID | P51906 |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:12119102, PubMed:18684713). Can also transport L-cysteine (PubMed:30840898). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:9233792). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:9233792). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (PubMed:30840898). Negatively regulated by ARL6IP5 (PubMed:12119102).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205239 | Slc1a1 (untagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1), (10ug) |
CNY 3,904.00 |
|
| MR208367 | Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 3,896.00 |
|
| MR208367L3 | Lenti ORF clone of Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 6,460.00 |
|
| MR208367L4 | Lenti ORF clone of Slc1a1 (mGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 6,460.00 |
