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Usp3 (NM_144937) Mouse Tagged ORF Clone
CAT＃: MG208332
- TrueORF®
Usp3 (tGFP-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3)
ORF Plasmid: DDK
"NM_144937" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409661; BC017156 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208332 representing NM_144937
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTCCGCACCTCAGCTCCAGCGTCTGCATCGCCCCGGACTCAGCCAAGTTCCCCAACGGCTCCC CGTCGTCCTGGTGCTGCAGCGTGTGCAGGTCCAACAAAAGCCCCTGGGTCTGTTTGACTTGTTCAAGTGT CCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAGATACCCTTACTCAAC CATAAGAGATCAGAAAAGCAGGAGAAAGCTCAGCATACAGTGTGTATGGATTGCAGTAGCTACAGCACAT ACTGTTATCGCTGTGACGATTTTGTGGTCAACGACACCAAGCTGGGCCTGGTACAGAAAGTGAGAGAACA CTTACAGAACTTGGAAAACTCAGCCTTTACAGCGGACAGGCACAGAAAAAGAAAATTATTGGAAAACTCA TCACTAAACAGCAAGTTATTAAAAGTAAATGGAAGTACGACTGCCATTTGTGCCACAGGCCTTCGAAATT TGGGCAACACTTGTTTCATGAATGCTATCCTTCAGTCACTCAGTAACATTGAGCAGTTTTGCTGTTATTT CAAAGAACTGCCTGCTGTGGAGTTAAGGAATGGGAAAACAGCAGGAAGGCGAACATACCACACCAGGAGC CAAGGGGACAGCAATGTGTCTTTAGTAGAAGAGTTTAGAAAGACACTGTGTGCTTTATGGCAAGGCAGCC AGACTGCATTTAGTCCTGAGTCCTTGTTTTATGTTGTCTGGAAGATCATGCCTAACTTTAGGGGTTATCA GCAGCAGGATGCCCATGAATTCATGCGCTACCTTTTGGATCATCTACACTTAGAACTTCAGGGTGGCTTC AATGGTGTTTCCCGCTCAGCAATTCTACAGGAGAATTCTACACTGTCTGCAAGTAACAAATGCTGCATAA ATGGAGCATCAACTGTTGTCACAGCAATATTTGGAGGAATTCTCCAGAACGAGGTCAACTGCCTCATATG TGGGACAGAGTCGAGGAAGTTTGATCCATTCCTAGATCTCTCCTTAGATATTCCAAGTCAGTTCAGAAGC AAGCGCTCTAAGAATCAGGAAAATGGGCCAGTTTGCTCCTTAAGAGATTGCCTTCGCAGCTTCACTGACC TAGAGGAACTTGATGAGACAGAGTTGTACATGTGCCATAAGTGCAAAAAGAAACAAAAGTCCACAAAGAA GTTTTGGATTCAAAAACTTCCCAAGGCGCTGTGCTTGCACCTGAAAAGGTTTCACTGGACAGCATATTTA AGAAACAAAGTCGATACATACGTTCAATTCCCACTGAGAGGCCTGGACATGAAGTGCTACCTACTAGAGC CGGAGAACAGTGGTCCAGACAGTTGCCTGTATGACCTCGCTGCTGTGGTTGTACACCATGGCTCTGGGGT TGGTTCTGGACATTACACAGCATATGCAGTTCACGAAGGCCGCTGGTTCCATTTCAATGACAGCACTGTG ACAGTGACTGATGAGGAGACCGTTGGGAAGGCAAAGGCCTACATTCTGTTTTATGTGGAGCGTCAGGCCA GAGCTGGAGCAGAAAAACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208332 representing NM_144937
Red=Cloning site Green=Tags(s) MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQIPLLN HKRSEKQEKAQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHRKRKLLENS SLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRS QGDSNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGF NGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKFDPFLDLSLDIPSQFRS KRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQKSTKKFWIQKLPKALCLHLKRFHWTAYL RNKVDTYVQFPLRGLDMKCYLLEPENSGPDSCLYDLAAVVVHHGSGVGSGHYTAYAVHEGRWFHFNDSTV TVTDEETVGKAKAYILFYVERQARAGAEKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144937 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144937.1 |
| RefSeq Size | 2202 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 235441 |
| UniProt ID | Q91W36 |
| Gene Summary | The protein encoded by this gene is a chromatin-associated histone 2A and 2B deubiquitinating enzyme that negatively regulates the DNA damage response. Mice deficient for this enzyme have reduced hematopoietic stem cell reserves, demonstrating a requirement in hematopoietic stem cell homeostasis. In addition, knock down of protein levels results in spontaneous tumor development and shortened lifespan, consistent with a function in preserving chromosomal integrity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201315 | Usp3 (untagged) - Mouse ubiquitin specific peptidase 3 (Usp3), (10ug) |
CNY 3,880.00 |
|
| MR208332 | Usp3 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3) |
CNY 3,880.00 |
|
| MR208332L3 | Lenti ORF clone of Usp3 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3) |
CNY 6,460.00 |
|
| MR208332L4 | Lenti ORF clone of Usp3 (mGFP-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3) |
CNY 6,460.00 |
