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Fus (NM_139149) Mouse Tagged ORF Clone
CAT＃: MG208306
- TrueORF®
Fus (tGFP-tagged) - Mouse fusion, derived from t(12;16) malignant liposarcoma (human) (Fus)
ORF Plasmid: DDK
"NM_139149" in other vectors (3)
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CNY 7,384.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D430004D17Rik; D930039C12Rik; Fus1; Tls |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208306 representing NM_139149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCAAACGACTATACCCAACAAGCAACTCAAAGCTACGGGGCCTATCCTACCCAGCCTGGGCAGG GCTACTCCCAACAGAGCAGTCAGCCCTATGGCCAACAGAGTTACAGTGGTTATGGCCAGTCAGCTGACAC TTCAGGATACGGCCAGAGCAGCTATGGTTCTTCTTATGGACAGACCCAAAACAGCTATGGAACTCAGTCA GCTCCCCAGGGATATGGTTCCACTGGAGGCTATGGCAGCAGCCAAAGTTCACAATCTTCTTATGGGCAGC AGTCCTCCTACCCTGGCTATGGCCAACAGCCAGCTCCTAGCAGCACCTCAGGAAGTTATGGTGGCAGTTC TCAGAGCAGCAGCTATGGGCAACCCCAGAGTGGAGGTTATGGTCAACAGTCTGGCTATGGTGGACAGCAA CAAAGCTACGGACAACAACAAAGCTCCTATAACCCACCTCAGGGTTATGGACAACAGAACCAGTACAACA GTAGCAGTGGAGGTGGTGGAGGGGGTGGTGGAGGCAACTATGGCCAAGATCAGTCCTCTATGAGTGGTGG CGGTGGCGGTGGTGGTTATGGCAATCAGGACCAGAGTGGTGGCGGCGGCGGCGGCTACGGGGGAGGCCAA CAGGATCGTGGGGGCCGAGGCAGGGGCGGTGGAGGTGGTTACAACCGAAGCAGTGGTGGCTATGAACCCA GAGGCCGTGGAGGTGGCCGAGGAGGCAGAGGCGGCATGGGCGGAAGTGACCGCGGTGGCTTCAATAAATT TGGTGGTCCTCGGGATCAAGGATCTCGTCATGATTCTGAACAGGATAATTCAGACAACAATACCATCTTC GTGCAAGGCCTAGGCGAGAATGTTACAATTGAATCTGTGGCTGATTACTTCAAGCAGATTGGAATTATTA AGACAAACAAGAAAACTGGACAGCCTATGATTAATTTGTACACAGACAGGGAAACTGGCAAGTTGAAGGG TGAGGCAACAGTTTCATTTGATGACCCACCTTCTGCTAAAGCAGCTATCGACTGGTTTGATGGTAAAGAA TTCTCTGGGAATCCTATTAAAGTTTCATTTGCTACCCGCCGAGCTGACTTCAATCGGGGTGGTGGAAATG GTCGCGGAGGCCGAGGGCGAGGAGGACCCATGGGCCGTGGAGGCTATGGAGGAGGTGGCAGTGGTGGTGG TGGCCGGGGAGGATTCCCCAGTGGAGGTGGTGGAGGTGGAGGACAGCAACGAGCTGGAGACTGGAAGTGT CCTAATCCTACATGTGAGAACATGAACTTCTCTTGGAGAAATGAATGCAACCAGTGTAAGGCACCTAAGC CAGATGGCCCAGGAGGGGGACCAGGAGGCTCTCATATGGGGGGAAACTATGGAGATGATCGACGTGGCAG AGGAGGATATGACCGGGGCGGCTACCGGGGCCGAGGAGGGGACCGTGGGGGCTTCAGAGGGGGCCGGGGT GGTGGGGACAGAGGCGGTTTTGGCCCTGGCAAGATGGACTCCAGGGGCGAGCACAGACAGGATCGCAGGG AGAGGCCATAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208306 representing NM_139149
Red=Cloning site Green=Tags(s) MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYGQSADTSGYGQSSYGSSYGQTQNSYGTQS APQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGGSSQSSSYGQPQSGGYGQQSGYGGQQ QSYGQQQSSYNPPQGYGQQNQYNSSSGGGGGGGGGNYGQDQSSMSGGGGGGGYGNQDQSGGGGGGYGGGQ QDRGGRGRGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIF VQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKE FSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKC PNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGRGGYDRGGYRGRGGDRGGFRGGRG GGDRGGFGPGKMDSRGEHRQDRRERPY TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139149 |
| ORF Size | 1551 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139149.2, NP_631888.1 |
| RefSeq Size | 1845 bp |
| RefSeq ORF | 1557 bp |
| Locus ID | 233908 |
| UniProt ID | P56959 |
| Gene Summary | DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (By similarity). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (PubMed:16317045, PubMed:25968143).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208306 | Fus (Myc-DDK-tagged) - Mouse fusion, derived from t(12,16) malignant liposarcoma (human) (Fus) |
CNY 5,784.00 |
|
| MR208306L3 | Lenti ORF clone of Fus (Myc-DDK-tagged) - Mouse fusion, derived from t(12;16) malignant liposarcoma (human) (Fus) |
CNY 7,030.00 |
|
| MR208306L4 | Lenti ORF clone of Fus (mGFP-tagged) - Mouse fusion, derived from t(12;16) malignant liposarcoma (human) (Fus) |
CNY 7,030.00 |
