Hbp1 (BC026853) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1700058O05Rik; BE629963; C86454; C330012F01Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208277 representing BC026853 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTGGGAAGTGAAGACAAATCAGATGCCTAATGCAGTACAGAAACTCCTGCTGGTGATGGACAAGA GAGCACCAGGGATGAGTGACTCGCTGGAGTTGCTGCAGTGTAATGAGAATTTGCCATCCTCTCCAGGATA CAACTCCTGTGATGAGCACATGGAGCTTGATGACCTTCCTGAACTTCAGGCTGTTCAAAGTGACCCTACC CAATCTGCCATATACCAGCTAAGTTCAGATGTATCTCATCAAGAATATCCAAGATCATCTTGGAGCCAGA ATACCTCAGATATACCGGAAAATACTCACCGTGAAGATGAGGTGGACTGGCTAACAGAGTTAGCAAATAT TGCCACTAGTCCACAAAGTCCCCTGATGCAGTGCTCATTTTATAACAGATCATCTCCTGTACACATCATA GCTACTAGCAAAAGTTTACATTCCTATGCACGCCCACCACCAGTGTCCTCTTCTTCAAAAAGTGGGCCAG CCTTCCCTCATGACCACTGGAAGGAAGAAACACCAGTAAGACATGAAAGGGCAAACAGTGAGTCAGAGTC AGGCATTTTCTGTATGTCCTCCCTGTCAGATGATGATGATCTGGGCTGGTGCAATTCCTGGCCATCCACC ATTTGGCACTGCTTTCTGAAAGGCACGCGGCTGTGCTTCCATAAGGAAAGCAATAAGGAATGGCAAGATG TGGAAGACTTTGCTAGAGCCGCCAGCTGTGATAATGAGGAAGAGATCCAAATGGGCACTCACAAGGGCTA TGGGTCTGATGGTCTAAAGTTGTTATCCCATGAAGAGAGTGTGTCATTCGGTGAGTCTGTACTGAAGTTG ACATTTGATCCTGGTACAGTAGAAGATGGCTTGCTCACTGTAGAGTGCAAGCTGGACCACCCTTTCTATG TTAAAAATAAAGGTTGGTCGTCATTTTATCCAAGCTTGACTGTGGTACAGCATGGCATTCCATGTTGTGA AATTCATATTGGTGACGTATGTCTACCTCCTGGACACCCTGATGCCATTAATTTCGATGATTCAGGTGTT TTTGATACATTTAAAAGCTATGACTTCACACCTATGGACTCTTCTGCGGTCTATGTTTTGAGTAGTATGG CTCGTCAGCGCCGTGCATCTCTGTCTTGTGGAGGAGGACCTGGTACTGGTCAAGAGTTTTCGGGATCTGA ATTCAGTAAAAGCTGTGGTTCACCTGGATCATCACAGCTCTCTTCTAGTTCTTTGTATGCTAAAGCTGTC AAAAGCCACAGCTCAGGGACTGTGAGTGCCACTTCTCCTAATAAGTGCAAAAGACCAATGAATGCCTTCA TGCTTTTTGCCAAAAAATACAGAGTTGAATATACTCAGATGTATCCAGGGAAAGATAACAGAGCCATAAG TGTGATCCTTGGTGACAGGTGGAAAAAAATGAAGAACGAAGAAAGGAGGATGTACACATTAGAAGCAAAG GCTTTGGCTGAAGAACAAAAGCGTTTAAATCCTGACTGTTGGAAAAGGAAAAGAACCAACTCAGGCTCTC AACAGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208277 representing BC026853 Red=Cloning site Green=Tags(s) MVWEVKTNQMPNAVQKLLLVMDKRAPGMSDSLELLQCNENLPSSPGYNSCDEHMELDDLPELQAVQSDPT QSAIYQLSSDVSHQEYPRSSWSQNTSDIPENTHREDEVDWLTELANIATSPQSPLMQCSFYNRSSPVHII ATSKSLHSYARPPPVSSSSKSGPAFPHDHWKEETPVRHERANSESESGIFCMSSLSDDDDLGWCNSWPST IWHCFLKGTRLCFHKESNKEWQDVEDFARAASCDNEEEIQMGTHKGYGSDGLKLLSHEESVSFGESVLKL TFDPGTVEDGLLTVECKLDHPFYVKNKGWSSFYPSLTVVQHGIPCCEIHIGDVCLPPGHPDAINFDDSGV FDTFKSYDFTPMDSSAVYVLSSMARQRRASLSCGGGPGTGQEFSGSEFSKSCGSPGSSQLSSSSLYAKAV KSHSSGTVSATSPNKCKRPMNAFMLFAKKYRVEYTQMYPGKDNRAISVILGDRWKKMKNEERRMYTLEAK ALAEEQKRLNPDCWKRKRTNSGSQQH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026853
ORF Size	1550 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026853, AAH26853
RefSeq Size	2642 bp
RefSeq ORF	1550 bp
Locus ID	73389
Gene Summary	Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4 (By similarity).[UniProtKB/Swiss-Prot Function]

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