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Toe1 (NM_026654) Mouse Tagged ORF Clone
CAT＃: MG208219
- TrueORF®
Toe1 (tGFP-tagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1)
ORF Plasmid: DDK
"NM_026654" in other vectors (4)
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CNY 5,416.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930584N22Rik; 4933424D16Rik; AI413517 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208219 representing NM_026654
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCAGACAGTGACGATGGTGTGCCTCCAGTTCCAACGTCCTCTGACGGTGGTGTCAACAAAAACA CACAATCTGCAGAGGAGTTTGTGGTTCGGGTTCCTGTGGTAGACGTGCAGAGTGACAACTTCAAGGAGAT ATGGCCATCACTCTTGCTAGCCTTAAAGACAGCTAGCTTTGTGGCTGTGGACACGGAGCTGAGTGGGCTC GGGGATAGGAAGAGTTTGCTGAACCAGTGCATTGAGGAACGTTACAAGGCCGTGTGTCATGCTGCCAGGA CCCGTTCTGTCCTGTCCCTGGGCCTCGCCTGCTTCAGGCAGCAACCAGATAAGGGTGAAAATTCTTACTT GGCTCAAGTGTTCAATCTCACCCTGCTATGCATGGAGGAGTACGTTATAGAACCGAAGTCTGTGCAGTTC CTGGTACAGCATGGCTTCAATTTCAACCGGCAGTACGCCCAGGGTATCCCCTACCATAAGGGCAATGACA AGGGAGATGAGAGCCAGAGCCAGTCGGTACGGACGCTGTTCCTTGAACTGATTCGGGCCCGACGGCCCCT GGTGCTACATAATGGCCTTATAGACTTGGTGTTCCTGTACCAGAATTTCTACGCGCACCTGCCTGAGAAC TTGGGGACCTTCACTGCTGACCTGTGTGAGATGTTCCCAGCAGGCATCTATGACACCAAATATGCCGCCG AGTTCCATGCCCGATTTGTAGCTTCCTACTTAGAATACGCTTTCCGGAAATGTGAGCGGGAGAATGGGAA GCAGCGGGCAGCTGGCAGCCCACATCTTGCCCTGGAATTCTGCAGCTATCCATCTAGCATGAGAGGGCAC ATTGACTACCGCTGCTGTATGTCCCCTGGGACCTGCCGTCGTTCTCGTACCACTGGCATCTGTGACAAGT TCTCGGCCTATGGCTGGTGTCCTCTGGGACCACAGTGTCCTCAGTCACACGATATTGACCTTATAATTGA CACTGATGAGGCTGTGGCAGAAGATAAGCGGCGGAGACGGCGACGTAAGGACAAACGGAAGAGGTCTTTA CAGAGCCAACCAGGGACACAGACCCTTGTGGAAGCTGAAGATGGTCCTCCCACAAAGCAGGTCTGTGAAG ATAGCCTCGAGACTGAGAAAATGGAACAAAAAGTGGCAGAAGGTGAGGCTGGGGATCAGCCTGGCTCCAG GGAAGGTCACACAAGTAGCTTGGAGATGGCACATAGGAGGACAAGTGCTGAAACAGCTGATGTGGCCACC TCAGAGCTGCTGGTGAACCAAGCCAGCACTAACCCCGTGCCTGGGGACGGACTGCATCGTGCTGGTTTTG ACGCCTTTATGACAGGCTATGTGATGGCCTATGTGGGCCTGAGCCAGGGACTTCAACTCTGCAGCTCTGA ACCTTGGCTTCCTGAATGTCACAACAAGGTATATCTAAGTGGCAAAACTGTACCCCTCACAGTGACCAAG AGCCAGTTTTCCCGTCCATCCAAAGCCCACAACCAGAAAATGAAGCTGGCTTGGGGCAGCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208219 representing NM_026654
Red=Cloning site Green=Tags(s) MAADSDDGVPPVPTSSDGGVNKNTQSAEEFVVRVPVVDVQSDNFKEIWPSLLLALKTASFVAVDTELSGL GDRKSLLNQCIEERYKAVCHAARTRSVLSLGLACFRQQPDKGENSYLAQVFNLTLLCMEEYVIEPKSVQF LVQHGFNFNRQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPEN LGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLALEFCSYPSSMRGH IDYRCCMSPGTCRRSRTTGICDKFSAYGWCPLGPQCPQSHDIDLIIDTDEAVAEDKRRRRRRKDKRKRSL QSQPGTQTLVEAEDGPPTKQVCEDSLETEKMEQKVAEGEAGDQPGSREGHTSSLEMAHRRTSAETADVAT SELLVNQASTNPVPGDGLHRAGFDAFMTGYVMAYVGLSQGLQLCSSEPWLPECHNKVYLSGKTVPLTVTK SQFSRPSKAHNQKMKLAWGSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026654 |
| ORF Size | 1533 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026654.2, NP_080930.1 |
| RefSeq Size | 2008 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 68276 |
| UniProt ID | Q9D2E2 |
| Gene Summary | Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217119 | Toe1 (untagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1), (10ug) |
CNY 4,192.00 |
|
| MR208219 | Toe1 (Myc-DDK-tagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1) |
CNY 3,816.00 |
|
| MR208219L3 | Lenti ORF clone of Toe1 (Myc-DDK-tagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1) |
CNY 6,460.00 |
|
| MR208219L4 | Lenti ORF clone of Toe1 (mGFP-tagged) - Mouse target of EGR1, member 1 (nuclear) (Toe1) |
CNY 6,460.00 |
