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Edc3 (NM_153799) Mouse Tagged ORF Clone
CAT＃: MG208161
- TrueORF®
Edc3 (tGFP-tagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3)
ORF Plasmid: DDK
"NM_153799" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA517853; Lsm16; Yjdc |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208161 representing NM_153799
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGGATTGGCTAGGCAGTATTGTGTCCATCAACTGTGGAGATAGCCTGGGCGTCTACCAGGGAA GAGTATCAGCAGTGGATCAGGTCAGCCAGACCATTTCTCTTACTCGGCCCTTCCACAATGGGGTGAAGTG TCTTGTACCAGAAGTCACCTTTCGGGCAGGTGATATTACAGAATTAAAAATTTTGGAGATACCAGGTCCT GGAGACAACCAGCAGGTTGGAGACCTTCATCAGACAGAATTAGGCCCTTCTGGTGTTGGGTACCAAATGA GTATCAGTCAGAATGGAACAGGCAAGGTGGTCAAGAAGCCAGCCTCTTCCAGTAGTGCCCCTCAGAGCAT CCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCATTTCTCCTCAGCAGCAGCAGTGCTCAAAGAGT TATGTGGACAGGCACATGGAATCTTTGAGTCAGTCCAAAAGCTTCCGTCGTCGGCACAACTCCTGGTCAT CTAGTAGCAGGCACCCAAACCAGGCAACTCCAAAGAAAAGTGGTTTAAAAAATGGCCAGATGAAGAATAA AGATGACGAGTGCTTTGGAGACGATATCGAGGAGCTCCCAGACACGGACTTTGACTTTGAGGGGAACCTG GCCCTGTTTGACAAGGCGGCTGTGTTTGAGGAGATCGACACTTACGAGAGGAGAAGTGGCTCCCGTTCTC GGGGTGTCCCAAATGAACGTCCTGCTAGGTACCGCCATGATGAAAACATCCTGGAATCGGAGCCGATTGT GTACCGTAGGATCACAGTACCACACAGCGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTAGTCCCA AGTGTTTCCTATGAGCTACATAAAAAGCTGTTGTCTGTGGCTGAGAAGCATGGGCTGACCTTAGAGCGGA GACTTGAGATGACTGGTGTGTGTGCCAGTCAGATGGCGCTGACTCTCCTTGGAGGACCCAACAGGTTGAA TCCTAAGAATGTCCACCAGAGGCCCACAGTAGCTCTCTTGTGCGGCCCCCATGTGAAGGGGGCTCAGGGC ATCAGTTGTGGGAGGCACCTCGCCAACCATGATGTGCAAGTCATCCTCTTCCTGCCCAACTTTGTCAAGA TGCTGGAGTCTATTACCAACGAGCTGTCTCTCTTCAGCAAGACCCAAGGGCAGCAAGTGTCTAGCCTAAG AGATCTCCCTGCTAGCCCTGTGGACCTGGTTATCAACTGCCTGGATTGTCCTGAGAATGCCTTCCTGCGG GATCAGCCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTGCTCAGCATAGACC CTCCTGTGCATGAAGTTGAACAGGGCATTGATGCCAAATGGTCCCTGGCACTGGGCCTGCCTCTGCCACT CGGGGAGCACGCAGGCCGAGTCTATTTGTGTGACATTGGCATTCCCCAGCAAGTCTTCCAGGAGGTTGGC ATCAACTACCATTCACCTTTTGGCTGCAAATTTGTCATTCCCCTACACTCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208161 representing NM_153799
Red=Cloning site Green=Tags(s) MAMDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPGP GDNQQVGDLHQTELGPSGVGYQMSISQNGTGKVVKKPASSSSAPQSIPKRTDVKSQDVAISPQQQQCSKS YVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEELPDTDFDFEGNL ALFDKAAVFEEIDTYERRSGSRSRGVPNERPARYRHDENILESEPIVYRRITVPHSVSKEFCTDSGLVVP SVSYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQG ISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLRDLPASPVDLVINCLDCPENAFLR DQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRVYLCDIGIPQQVFQEVG INYHSPFGCKFVIPLHSA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153799 |
| ORF Size | 1524 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153799.3, NP_722494.1 |
| RefSeq Size | 3391 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 353190 |
| UniProt ID | Q8K2D3 |
| Gene Summary | Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217226 | Edc3 (untagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3), (10ug) |
CNY 6,560.00 |
|
| MR208161 | Edc3 (Myc-DDK-tagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3) |
CNY 3,792.00 |
|
| MR208161L3 | Lenti ORF clone of Edc3 (Myc-DDK-tagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3) |
CNY 6,370.00 |
|
| MR208161L4 | Lenti ORF clone of Edc3 (mGFP-tagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3) |
CNY 6,370.00 |
