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Shmt2 (NM_028230) Mouse Tagged ORF Clone
CAT＃: MG208109
- TrueORF®
Shmt2 (tGFP-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2)
ORF Plasmid: DDK
"NM_028230" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2700043D08Rik; AA408223; AA986903 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208109 representing NM_028230
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATCCTTCTCTTTGCTTCGGACCACTCGGCCTCTGCAGAGATGTGGCCAGCTGGTCTGCATGGCTG CCCGGGCCCAGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAACTGGAGGTTGGACGGGCCAGGA GAGTTTATCAGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAGACAGTGTCGCGGC CTGGAGCTCATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGGTCCTGTCTCAACA ACAAGTACTCGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGGACGAGATCGAGCT GCTCTGCCAGCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGTCAATGTGCAGCCA TACTCAGGGTCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGATCGAATCATGGGGT TGGACCTGCCCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGATCTCCGCCACATC CATTTTCTTCGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTACGACCAGCTGGCG CTGACCGCTCGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCCCGCCTCATTGACT ATGCACGCATGAAAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGGCCCACATCAGTGG CCTGGTGGCTGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCACCACCACTCACAAG ACACTGCGAGGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTAGACCCCAAGACTG GCAAAGAGATCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCCTACAGGGTGGCCC CCACAACCACGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTATGTTCCGCGAGTAC TCCTTACAAGTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGATACTCGCTGGTGT CTGGTGGCACTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATGGAGCCCGAGCCGA ACGCGTGTTGGAACTTGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCGGAGCGCCATTACT CCGGGGGGCTTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGACGACTTCCGTAGAG TCGTCGATTTTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTGCCAAGCTCCAGGA TTTCAAATCCTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCGGCAACAGGTGGAA CAGTTTGCCAGGGGCTTCCCGATGCCTGGATTTGATGAACGT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208109 representing NM_028230
Red=Cloning site Green=Tags(s) MVSFSLLRTTRPLQRCGQLVCMAARAQHSKVAQTQAGEATGGWTGQESLSDSDPEMWELLQREKDRQCRG LELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQP YSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLA LTARLFRPRLIIAGTSAYARLIDYARMKEVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHK TLRGARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREY SLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAIT PGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVE QFARGFPMPGFDER SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028230 |
| ORF Size | 1512 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028230.3, NP_082506.1 |
| RefSeq Size | 2280 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 108037 |
| UniProt ID | Q9CZN7 |
| Gene Summary | Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (By similarity). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (By similarity). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (By similarity). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640). Associates with mitochondrial DNA (By similarity). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202532 | Shmt2 (untagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR208109 | Shmt2 (Myc-DDK-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein |
CNY 3,760.00 |
|
| MR208109L3 | Lenti ORF clone of Shmt2 (Myc-DDK-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein |
CNY 6,370.00 |
|
| MR208109L4 | Lenti ORF clone of Shmt2 (mGFP-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein |
CNY 6,370.00 |
