Plcd1 (BC015249) Mouse Tagged ORF Clone
CAT#: MG208093
- TrueORF®
Plcd1 (tGFP-tagged) - Mouse phospholipase C, delta 1 (cDNA clone MGC:18330 IMAGE:4241580)
ORF Plasmid: DDK
"BC015249" in other vectors (3)
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CNY 5,510.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AW212592; C79986 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208093 representing BC015249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCGGTCGGGACTTCCTGACCTTGCACGGGCTGCAGGATGACCCGGACCTTCAGGCCCTCCTGA AGGGCAGCCAGCTTCTGAAGGTGAAATCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTACAGGAGGA CTGCAAGACCATCTGGCAGGAATCTCGCAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCTCCATCGAG GACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACATACCCGAGG ACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCTCCAGCTGA CGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGCGGCAGAAG CTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAACTTCAAGG AGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATCTTCAGGGA ATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGCTGACCCAG CGGGCAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCGGAGACCCTGTCGGTGGAGAAGCTAG TGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTCATTGAGCG CTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGTACCTGCTG TCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCCGCTCAGCC ACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGCAGCACGGA GGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCCCCAACCAG GAACCCATCATCTACCACGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAGGGCCATCC GGGACTACGCCTTCAAGGCATCCCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGCCTGGAGCA ACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGCTGGATGGG GTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAAACTGGGCG GGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAGGCTGCTGA AATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGTGCCCACCTTGTCCTACCCTACCT ATGGCTCTAACCTGGGCCTCCCCGCGGCCCCCAGGAGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208093 representing BC015249
Red=Cloning site Green=Tags(s) MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIE DIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQK LQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQ RAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLL SADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQ EPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDG VTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKCPPCPTLP MALTWASPRPPGG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC015249 |
ORF Size | 1509 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC015249.1 |
RefSeq Size | 2674 bp |
RefSeq ORF | 1511 bp |
Locus ID | 18799 |
Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR208093 | Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (cDNA clone MGC:18330 IMAGE:4241580) |
CNY 5,040.00 |
|
MR208093L3 | Lenti ORF clone of Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (cDNA clone MGC:18330 IMAGE:4241580) |
CNY 6,940.00 |
|
MR208093L4 | Lenti ORF clone of Plcd1 (mGFP-tagged) - Mouse phospholipase C, delta 1 (cDNA clone MGC:18330 IMAGE:4241580) |
CNY 6,940.00 |