5730410E15Rik (BC048945) Mouse Tagged ORF Clone
CAT#: MG207983
- TrueORF®
5730410E15Rik (tGFP-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420)
ORF Plasmid: DDK
"BC048945" in other vectors (4)
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CNY 3,140.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Golsyn |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207983 representing BC048945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACGACAGGAAACAAGAGAGCCTCTTTCTCACGCAACCGAGGCCCTCATGGGCGGAGCAATGGAG CATCATCCCACAAGTCTGGCAGCAGCCCACCATCCCCAAGGGAAAAAGACCTTGTGTCTATGCTGTGCAG GAATCCACTGAGCCCCAGTAACATCCACCCTAGTTACGCCCCTTCTTCTCCAAGTAGCAGCAACTCCGGC TCCTACAAAGGAAGTGATTGTAGTCCAGTCATGAGAAGGTCTGGAAGATACATGTCTTGTGGCGAAAATC ACGGTGTCAAACCCCCAAATCCAGAACAGTATTTGACTCCTCTGCAGCAGAAGGAGGTCACAGTGAGGCA CCTGAGGACCAAGCTGAAGGAGTCTGAGCGCCGACTCCATGAGAGGGAATCTGAAATCATGGAGCTCAAG TCTCAGCTGGCTCGAATGAGGGAGGACTGGATTGAGGAAGAGTGCCACAGAGTGGAGGCTCAGTTGGCAC TCAAAGAAGCCAGAAAAGAGATTAAACAGCTCAAACAGGTCATCGAGACTATGAGGAGCAGCTTGGCTGA TAAAGATAAAGGCATTCAGAAGTACTTTGTGGACATAAACATCCAAAACAAGAAACTGGAGTCTCTGCTT CAAAGCATGGAGATGGCACACAATAGTTCCCTGAGGGATGAACTGTGTCTTGACTTTTCCTTTGATTCCC CAGAGAAAAGTTTACCCCTGAGCAGCACATTTGACAAGTTGCCAGATGGGTTATCTCTGGAAGAACAGAT AACAGAGGAAGGTGCTGACAGTGAGCTTCTGGTGGGAGACAGCATGGCTGAAGGCACAGATCTGTTAGAT GAGATGGTGACTGCCACCACCACAGAATCCAGTGGCCTGGAGTTTGTTCATTCCACTCCAGGGCCACAAG CCCTCAAGGCTCTCCCCTTGGTGAGCCACGAAGAGGGCATTGCGGTGATGGAGCAAGCCGTGCAGACCGA CGTGGTGCCGTTCAGCCCTGCCATCTCAGAGCTCATTCAGAGTGTGCTAAAGCTGCAGGACTACTGTCCC ACAAGCTCAGCATCTCCAGATGAATCTGGAGCTGACTCGATGGAAAGCTTCTCAGAATCTATCTCTGCCT TAATGCTTGATTTAACTCCAAGAAGTCCTAACTCGGCCATCCTTCTGTCTCCTGTGGAGATCCCATTCAG CAAGGGGGCTATGGAAGCCCATGCAAATCGCCTCATGAGAGAGCTAGATTTTGCAGCCTACACAGAAGAA AGATTGGACAGTGTCCTCTCACTGTCCCAGGGCAGTGTTGTGAGGCAGTACTGGAGCAGCAATTTCTTGG TGGATCTACTGGCTGTGGCTGCCCCTGTGGTACCCACTGTTCTGTGGGCATTCAGTACTCAGAGAGGGGG TACAGATCCTGTCTACAACATCGGAGCTTTGCTCCGAGGTTGCTGTGTGGTGGCCCTACACTCACTACGC CGCACCGCTTTCCACATGAAAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207983 representing BC048945
Red=Cloning site Green=Tags(s) MSTTGNKRASFSRNRGPHGRSNGASSHKSGSSPPSPREKDLVSMLCRNPLSPSNIHPSYAPSSPSSSNSG SYKGSDCSPVMRRSGRYMSCGENHGVKPPNPEQYLTPLQQKEVTVRHLRTKLKESERRLHERESEIMELK SQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSLADKDKGIQKYFVDINIQNKKLESLL QSMEMAHNSSLRDELCLDFSFDSPEKSLPLSSTFDKLPDGLSLEEQITEEGADSELLVGDSMAEGTDLLD EMVTATTTESSGLEFVHSTPGPQALKALPLVSHEEGIAVMEQAVQTDVVPFSPAISELIQSVLKLQDYCP TSSASPDESGADSMESFSESISALMLDLTPRSPNSAILLSPVEIPFSKGAMEAHANRLMRELDFAAYTEE RLDSVLSLSQGSVVRQYWSSNFLVDLLAVAAPVVPTVLWAFSTQRGGTDPVYNIGALLRGCCVVALHSLR RTAFHMKT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC048945 |
ORF Size | 1496 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC048945, AAH48945 |
RefSeq Size | 2679 bp |
RefSeq ORF | 1496 bp |
Locus ID | 319613 |
Gene Summary | Part of a kinesin motor-adapter complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218048 | 5730410E15Rik (untagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420), (10ug) |
CNY 6,940.00 |
|
MR207983 | 5730410E15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) |
CNY 3,656.00 |
|
MR207983L3 | Lenti ORF clone of 5730410E15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) |
CNY 4,750.00 |
|
MR207983L4 | Lenti ORF clone of 5730410E15Rik (mGFP-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) |
CNY 4,750.00 |
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