Camk2g (BC025597) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Camkg
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207945 representing BC025597 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCACCGCCACCTGCACCCGCTTCACCGACGACTACCAGCTTTTCGAGGAGCTCGGCAAGGGTG CCTTCTCAGTGGTCCGGAGGTGTGTAAAGAAAACATCTACGCAGGAATATGCTGCAAAAATCATCAATAC CAAGAAGTTGTCCGCCCGAGATCATCAGAAACTAGAACGAGAGGCCCGGATATGCCGACTTCTGAAACAT CCAAACATTGTGCGTCTCCATGACAGTATTTCTGAAGAAGGGTTTCACTACCTCGTGTTTGACCTTGTTA CCGGAGGGGAGTTGTTTGAAGACATTGTGGCCAGAGAGTACTACAGTGAAGCTGATGCCAGCCACTGTAT ACATCAGATCCTGGAGAGTGTTAACCACATCCACCAGCATGACATCGTCCACCGGGATCTGAAGCCTGAG AACTTGCTGCTGGCGAGTAAATGCAAGGGTGCTGCGGTCAAGCTAGCTGATTTTGGCCTGGCCATCGAAG TACAGGGAGAGCAGCAGGCTTGGTTTGGTTTTGCCGGCACCCCAGGTTACTTGTCCCCTGAGGTCTTGAG GAAAGATCCCTATGGAAAACCTGTGGACATCTGGGCCTGCGGGGTCATCCTGTATATCCTCCTGGTGGGC TACCCTCCCTTCTGGGATGAGGATCAGCACAAGCTGTATCAGCAGATCAAAGCTGGAGCCTACGATTTCC CATCACCAGAATGGGACACAGTCACTCCTGAAGCTAAGAACTTGATCAACCAGATGCTGACCATAAACCC TGCAAAGCGCATCACGGCCGACCAGGCTCTCAAGCACCCATGGGTCTGTCAACGGTCTACGGTGGCATCC ATGATGCATCGCCAAGAGACGGTAGAGTGCTTACGCAAATTCAACGCCCGGAGAAAACTGAAGGGTGCCA TCCTCACAACCATGCTTGTCTCCAGGAACTTTTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCAGATGG CGGTGTCAAGGAACCACAAACCACCGTGGTACATAATGCTACAGATGGGATCAAGGGCTCCACAGAGAGC TGTAACACCACTACAGAAGACGAAGATCTCAAAGTGAGAAAACAAGAAATCATTAAGATCACAGAACAAC TGATCGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGACCCGGGCCTCACATCCTT TGAGCCAGAAGCCCTTGGTAACCTCGTGGAAGGAATGGATTTCCATAAGTTTTACTTTGAGAATCTCCTG TCCAAGAACAGCAAGCCTATCCACACCACCATCCTAAACCCTCACGTCCACGTGATTGGGGAGGACGCAG CTTGCATCGCCTATATCCGGCTCACTCAGTACATCGACGGGCAGGGTCGGCCTCGCACCAGTCAGTCAGA GGAGACCCGGGTGTGGCACCGCCGGGATGGCAAGTGGCTCAATGTCCACTATCACTGCTCAGGGGCCCCT GCCGCACCGCTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207945 representing BC025597 Red=Cloning site Green=Tags(s) MATTATCTRFTDDYQLFEELGKGAFSVVRRCVKKTSTQEYAAKIINTKKLSARDHQKLEREARICRLLKH PNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIHQILESVNHIHQHDIVHRDLKPE NLLLASKCKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWACGVILYILLVG YPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVAS MMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSAAKSLLNKKSDGGVKEPQTTVVHNATDGIKGSTES CNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLL SKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAP AAPLQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025597
ORF Size	1487 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025597, AAH25597
RefSeq Size	3572 bp
RefSeq ORF	1487 bp
Locus ID	12325
Gene Summary	Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning (By similarity).[UniProtKB/Swiss-Prot Function]

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