Dym (BC018220) Mouse Tagged ORF Clone

CAT#: MG207868

  • TrueORF®

Dym (tGFP-tagged) - Mouse dymeclin (cDNA clone MGC:18957 IMAGE:3985017)

ORF Plasmid: DDK tGFP



  "BC018220" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,420.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC18957
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207868 representing BC018220
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGTCTTCCTTTCCTGCCAACTCTTCCACAAGGAGGTTCTGCGACAGAGCATCAGTCACAAGTACC
TGATGCAAGGCCCATGTCTTCCCTACACCAGTAAACTTGTGAAAACCTTATTGTATAACTTTATCAGACA
AGAAAAGCCACCTCCGCCCGGAACCCATGTCTTCCCTCAGCAGTCGGATGGGGGAGGACTGCTGTATGGA
CTGGCATCAGGAGTAGCAACTGGTCTCTGGACTGTCTTCACGCTAGGCGGGGCAGGCAGCAAAGCAGCTG
CGTCCCCAGAACTTACATCTCCTCTGGCCAACCAGAGTCTCCTGCTGCTGCTGGTGCTGGTGAACCTGAC
GGATGCCCCAGACATACCCAACCCCTACAGGCAAGCTGTCTCGTCATTTAAGAACACGCAAGACAGCAGT
CCGTTCCCATCATCAATTCCTCATACCTTCCAGATTAACTTTAACAGTTTGTACACAGCCCTGTGTGAGC
AGCAGACGTCTGATCAGGCAACGCTGCTCCTGTACACGCTGCTCCACCAGAACAGCAATGTCAGGACGTA
CGTGCTGGCCCGGACGGATATGGAAAATCTTGTTTTACCAATTCTTGAGATTTTGTATCATGTTGAGGAA
AGAAACTCACACCATGTCTATATGGCCCTTATAATCCTGTTGATCCTTACAGAAGACGATGGCTTCAATC
GGTCCATTCATGAAGTGATATTAAGAAACATTACTTGGTATTCAGAAAGAGTCTTAACTGAGATTTCCCT
GGGGAGCCTCCTAATTCTGGTGGTAATAAGAACCATCCAGTACAATATGACCAGGACTCGAGACAAGTAC
CTGCACACAAACTGCCTGGCGGCTTTAGCAAACATGTCAGCGCAGTTCCGCTCCCTCCACCAGTACGCTG
CCCAGAGGATCATCAGTTTGTTTTCTTTGCTGTCTAAAAAACACAACAAGGTCCTGGAGCAAGCCACGAA
GTCCTTGAGAGGTCCCCTGAGCTCCAGCGATGTCCCTCTCCCAGATTATGCACAGGACCTCAGTGTCATT
GAAGAAGTGATTCGGATGATGCTGGAGATCATCAACTCCTGCCTGACAAATTCTCTTCACCACAACCCAA
ACTTGGTGTACGCCTTGCTTTACAAACGGGACCTCTTTGAGCAATTCCGAACTCATCCCTCATTCCAGGA
CATAATGCAAAACATTGACCTGGTGATCTCCTTCTTTAGCTCAAGACTTCTACAAGCTGGAGCTGAGCTG
TCGGTGGAAAGGGTCTTGGAAATCATTAAACAAGGGGTTGTGGCGCTGCCCAAAGACAGACTAAAGAAGT
TTCCAGAACTGAAGTTCAAATACGTGGAGGAAGAGCAGCCTGAGGAGTTCTTCATCCCGTACGTCTGGTC
CCTGGTCTACAACTCAGCGGTCGGCTTGTACTGGAACCCGCAGGACATCCAGCTGTTTGCCATGGACTCG
GAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207868 representing BC018220
Red=Cloning site Green=Tags(s)

MVVFLSCQLFHKEVLRQSISHKYLMQGPCLPYTSKLVKTLLYNFIRQEKPPPPGTHVFPQQSDGGGLLYG
LASGVATGLWTVFTLGGAGSKAAASPELTSPLANQSLLLLLVLVNLTDAPDIPNPYRQAVSSFKNTQDSS
PFPSSIPHTFQINFNSLYTALCEQQTSDQATLLLYTLLHQNSNVRTYVLARTDMENLVLPILEILYHVEE
RNSHHVYMALIILLILTEDDGFNRSIHEVILRNITWYSERVLTEISLGSLLILVVIRTIQYNMTRTRDKY
LHTNCLAALANMSAQFRSLHQYAAQRIISLFSLLSKKHNKVLEQATKSLRGPLSSSDVPLPDYAQDLSVI
EEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRTHPSFQDIMQNIDLVISFFSSRLLQAGAEL
SVERVLEIIKQGVVALPKDRLKKFPELKFKYVEEEQPEEFFIPYVWSLVYNSAVGLYWNPQDIQLFAMDS
D

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC018220
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC018220.1
RefSeq Size 1857 bp
RefSeq ORF 1475 bp
Locus ID 69190
Gene Summary Necessary for correct organization of Golgi apparatus. Involved in bone development.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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