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Dph2 (NM_026344) Mouse Tagged ORF Clone
CAT＃: MG207826
- TrueORF®
Dph2 (tGFP-tagged) - Mouse DPH2 homolog (S. cerevisiae) (Dph2)
ORF Plasmid: DDK
"NM_026344" in other vectors (4)
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CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9130020C19Rik; AI467389; Dph2l2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207826 representing NM_026344
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTACGTTCAGCAGCCCTGCGGAGGCAGCCCTGCAACGGGAGGCGGGCGTTCCAGGACAGTTCA CTCCTCCTGAAGACCTGGACCGCGTGTATGAGCTGGAGCGAGTTACGAAATTTGTCTGCGATTTAGGTTG TCAGCGGGTGACTTTGCAGTTCCCTGACCAGTTACTAGGAGATGCGGGAGCGGTGGCTGCCCGGCTGGAG GAAGTCACAGGAGCTAAGATGTTCATTTTAGGGGACACAGCTTATGGCAGCTGCTGTGTGGATGTGCTGG GCGCCGAGCAGGCTGGAGCTCAAGCCCTTGTGCACTTCGGTCCTGCCTGCTTAAGCCCCCCAGCCTCGCA GCTGCCCATCACCTTTGTCCTTGGTCAGCGACCCGTTGCTTTAGAGCTCTGTGCAAAGGCCTTTGAAGCC CAGAACCCAGATCCGACAGCCCCGGTGGTACTGCTGAGTGAGCCAGCTTGTGCCCATGCCCTAGAGCCTT TGGCCATGCTCCTGCTCCCAAAGTACCAAGATCTGCTCATCTCCCGCCCAGCTCTTCCCCTGCCAGTGGG ATCCCCGAGCTCACAGCCTGAGTCCCTGGAGCGTTTTGGGCGCTGCTTCCCCCTGAATCCAGGGAGACGT CTGGAAGAATATGGTGCCTTCTATGTAGGGGCTTCTCAAGCAAGCTCGGACTCCAGCCTTGATCCCGACC TGAGCAGACTGCTCTTGGGTTGGACACCAGGGCGGCCCTTCTTTTCCTGTTGTCCAGATACAGGACAGAC ACAAGACCAGGGTGCCAAGGCTGGGAGACTAAGAGCACGAAGACTGTATCTCATAGAGAGGGCCAGAGAT GCCCGTGTGGTTGGGCTGCTGGCAGGCATATTAGGTGTGGCTCGACACCGTGAGGCACTGGCACACTTGC GGAAACTGACGGAAGCTGCTGGAAAACGTAGCTATGTGTTAGCCGTGGGGAAGCCCACACCCGCCAAGCT TGCCAACTTCCCTGAGATGGACGTCTTTGTGCTGTTAGCCTGTCCCCTGGGGGCACTAGCCCCCCAGCCT TCGGGTGGCTTCTTTCGGCCTGTATTGACGCCATGTGAATTGGAGGCTGCCTGCAACCCTGCCTGGCCCC CGCCAGGCCTGGCTCCCCACCTCACACATTATGCAGAGCTGTTGCCTGGTTCTCCCTTCCATGTGCCACT CCCTCCACCTGAGTCAGAGTTGTGGGATACCCCAGATGTGTCACTCATTTCTGGGGAGCTCCGACCACCA CCTCCTTGGAAGTCATCAGATGACACTAGATGTTCGGCCTTAATTCCGAGGCCCCAACTGGAGCTGGCGG AGAGCAGCCCTGCAGCTTCATTCCTTAGTTCTCGGAACTGGCAGGGGCTGGAGCCACGCTTGGGCCAGAC ACCAGTGAAAGAAGCCGTCAGAGGAAGACGAGGTATCGCCATCGCCTACGAGGATGAGGGGAGCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207826 representing NM_026344
Red=Cloning site Green=Tags(s) MESTFSSPAEAALQREAGVPGQFTPPEDLDRVYELERVTKFVCDLGCQRVTLQFPDQLLGDAGAVAARLE EVTGAKMFILGDTAYGSCCVDVLGAEQAGAQALVHFGPACLSPPASQLPITFVLGQRPVALELCAKAFEA QNPDPTAPVVLLSEPACAHALEPLAMLLLPKYQDLLISRPALPLPVGSPSSQPESLERFGRCFPLNPGRR LEEYGAFYVGASQASSDSSLDPDLSRLLLGWTPGRPFFSCCPDTGQTQDQGAKAGRLRARRLYLIERARD ARVVGLLAGILGVARHREALAHLRKLTEAAGKRSYVLAVGKPTPAKLANFPEMDVFVLLACPLGALAPQP SGGFFRPVLTPCELEAACNPAWPPPGLAPHLTHYAELLPGSPFHVPLPPPESELWDTPDVSLISGELRPP PPWKSSDDTRCSALIPRPQLELAESSPAASFLSSRNWQGLEPRLGQTPVKEAVRGRRGIAIAYEDEGSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026344 |
| ORF Size | 1467 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026344.2, NP_080620.1 |
| RefSeq Size | 2527 bp |
| RefSeq ORF | 1470 bp |
| Locus ID | 67728 |
| UniProt ID | Q9CR25 |
| Gene Summary | Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201838 | Dph2 (untagged) - Mouse DPH2 homolog (S. cerevisiae) (Dph2), (10ug) |
CNY 2,000.00 |
|
| MR207826 | Dph2 (Myc-DDK-tagged) - Mouse DPH2 homolog (S. cerevisiae) (Dph2) |
CNY 3,656.00 |
|
| MR207826L3 | Lenti ORF clone of Dph2 (Myc-DDK-tagged) - Mouse DPH2 homolog (S. cerevisiae) (Dph2) |
CNY 6,180.00 |
|
| MR207826L4 | Lenti ORF clone of Dph2 (mGFP-tagged) - Mouse DPH2 homolog (S. cerevisiae) (Dph2) |
CNY 6,180.00 |
