Slc39a14 (BC021530) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC38539, fad123
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207824 representing BC021530 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGCTGCACCCAGCCCTTCCAAGCTGCCTCCTGTTGGTGCTGTTTGGTATATGGAGAACTGCCC CCCAGACTCATGCCTCGTCTGCTGGCCTGCCGCCCCTCAGTGCCACCTCCTTCCTGGAAGATCTCATGGA CCGCTATGGAAAGAATGACAGCCTTACCCTGACACAGCTGAAGTCCCTGCTCGACCACCTGCATGTGGGA GTGGGCCGGGATAATGTTTCCCAGCCCAAGGAAGGACCCAGGAACCTCTCCACGTGCTTTAGCTCTGGAG ACCTCTTTGCGGCGCACAACTTGAGCGAGCGATCTCAGATCGGGGCGAGTGAGTTCCAGGAGTTCTGCCC CACCATTCTCCAGCAGCTGGATTCCCAGGCCTGCACCTCAGAAAACCAGAAGTCTGAGGAGAATGAACAG ACAGAGGAGGGGAAGCCAAGCGCCATTGAAGTGTGGGGCTTTGGGTTCCTCAGTGTCTCACTGATTAACC TGGCCTCTCTCCTGGGAGTCCTGGTCCTGCCGTGCACGGAGAAAGCGTTTTTCAGCCGTGTGCTCACTTA CTTCATCGCCCTGTCCATTGGAACGCTGCTCTCTAACGCCCTTTTCCAGCTCATCCCAGAGGCTTTTGGC TTCAACCCTCAGGACAATTACGTCTCCAAGTCTGCAGTGGTGTTTGGGGGTTTCTACCTTTTCTTTTTCA CAGAAAAGATCCTGAAGATGCTCTTGAAGCAGAAAAACGAGCACCATCACGGGCATAACCATTTTACCTC CGAGACACTTCCTTCCAAGAAGGACCAGGAGGAGGGTGTGACCGAGAAGCTACAGAACGGGGACCTGGAT CACATGATCCCTCAGCACTGCAACAGCGAGCTGGATGGCAAGGCGCCTGGCACGGACGAGAAGGTCATTG TCAACTCCATGTCTGTGCAGGACCTGCAGGCATCCCAGAGTGCTTGCTACTGGCTCAAGGGGGTCCGCTA CTCTGATATCGGGACCTTGGCCTGGATGATCACCCTGAGCGATGGGCTCCACAATTTCATCGATGGCCTG GCTATTGGTGCCTCCTTCACTGTGTCTGTCTTCCAAGGCATCAGCACGTCAGTGGCCATTCTCTGTGAGG AATTCCCTCATGAGCTGGGAGACTTCGTCATCCTGCTCAATGCTGGCATGAGCATCCAGCAGGCTCTCTT CTTCAACTTCCTCTCTGCCTGCTGCTGCTATTTGGGTCTGGCCTTTGGCATCCTGGCTGGCAGCCACTTC TCTGCAAACTGGATTTTTGCACTGGCTGGAGGAATGTTCTTGTATATTGCCCTAGCCGATATGTTCCCTG AGATGAATGAAGTCTGCCAGGAGGATGAGAAGAACGACAGCTTTCTGGTCCCCTTTGTCATCCAGAATCT TGGCCTCCTAACCGGCTTCTCCATTATGCTGGTCCTCACAATGTATTCAGGGCAGATTCAGATCGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207824 representing BC021530 Red=Cloning site Green=Tags(s) MKRLHPALPSCLLLVLFGIWRTAPQTHASSAGLPPLSATSFLEDLMDRYGKNDSLTLTQLKSLLDHLHVG VGRDNVSQPKEGPRNLSTCFSSGDLFAAHNLSERSQIGASEFQEFCPTILQQLDSQACTSENQKSEENEQ TEEGKPSAIEVWGFGFLSVSLINLASLLGVLVLPCTEKAFFSRVLTYFIALSIGTLLSNALFQLIPEAFG FNPQDNYVSKSAVVFGGFYLFFFTEKILKMLLKQKNEHHHGHNHFTSETLPSKKDQEEGVTEKLQNGDLD HMIPQHCNSELDGKAPGTDEKVIVNSMSVQDLQASQSACYWLKGVRYSDIGTLAWMITLSDGLHNFIDGL AIGASFTVSVFQGISTSVAILCEEFPHELGDFVILLNAGMSIQQALFFNFLSACCCYLGLAFGILAGSHF SANWIFALAGGMFLYIALADMFPEMNEVCQEDEKNDSFLVPFVIQNLGLLTGFSIMLVLTMYSGQIQIG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021530
ORF Size	1469 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021530, AAH21530
RefSeq Size	2174 bp
RefSeq ORF	1469 bp
Locus ID	213053
Gene Summary	Broad-scope metal ion transporter with a preference for zinc uptake. Also mediates cellular uptake of nontransferrin-bound iron.[UniProtKB/Swiss-Prot Function]

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