Steap3 (BC037435) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Tsap6, pHyde
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207812 representing BC037435 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGGGAGATGGACAAGCCGCTGATCAGCCGCCGCCTAGTGGACAGTGATGGCAGTCTGGCTGAGG TCCCCAAGGAGGCCCCCAAAGTGGGCATCCTGGGCAGTGGGGATTTTGCCCGTTCCCTGGCCACACGCCT GGTGGGCTCTGGCTTCAGTGTGGTGGTGGGGAGCCGTAACCCCAAACGCACGGCTGGCCTCTTCCCCTCC TTAGCTCAAGTGACTTTCCAGGAGGAAGCCGTGAGCTCTCCAGAGGTCATCTTTGTGGCCGTGTTCCGGG AGCACTATTCCTCTCTGTGCAGTCTCGCTGACCAGTTGGCTGGCAAGATCCTCGTGGATGTAAGCAACCC CACGGAGAAGGAGCATCTTCAGCACCGCCAGTCTAACGCTGAGTACCTGGCCTCACTCTTTCCTGCGTGC ACTGTGGTGAAGGCCTTCAACGTCATCTCTGCATGGGCCCTACAGGCTGGCCCAAGGGATGGGAACAGGC AGGTGCTCATCTGCAGTGATCAGCCAGAAGCCAAGCGCACCATCTCAGAGATGGCACGCGCCATGGGTTT CACACCCCTGGACATGGGATCCCTGGCCTCAGCGCGGGAGGTAGAAGCCATACCCCTGCGCCTCCTTCCA TCCTGGAAGGTGCCCACCCTCCTGGCACTGGGGCTTTTTGTGTGCTTCTACACCTACAACTTCATCCGGG ACGTTCTACAGCCATACATTCGGAAAGATGAGAACAAGTTCTACAAGATGCCCTTGTCTGTGGTCAACAC CACACTACCCTGTGTGGCTTATGTGCTGCTGTCCCTAGTGTACCTGCCCGGTGTGCTGGCAGCTGCGCTT CAGCTGCGGAGGGGGACCAAGTACCAGCGTTTCCCAGACTGGCTGGACCACTGGCTGCAGCATCGCAAGC AGATCGGGCTGCTCAGCTTCTTCTTCGCGATGCTGCACGCTCTCTACAGCTTCTGCCTGCCGCTGCGCCG CTCCCACCGCTATGACCTGGTCAATCTGGCTGTGAAGCAGGTCCTGGCCAACAAGAGCCGCCTCTGGGTT GAGGAAGAAGTCTGGAGGATGGAGATATACCTGTCCCTGGGTGTGCTGGCCCTGGGCATGTTGTCGCTGC TGGCTGTCACCTCGCTCCCGTCCATTGCTAATTCCCTCAACTGGAAGGAGTTCAGCTTTGTGCAGTCCAC ACTGGGCTTCGTGGCCCTGATACTCAGCACAATGCACACACTCACCTACGGCTGGACCCGTGCCTTTGAG GAAAACCACTACAAGTTCTACCTGCCGCCCACATTCACACTCACGCTGCTCCTGCCCTGTGTGATCATCC TGGCCAAGGGCCTCTTCCTCCTGCCCTGCCTCAGCCGCAGACTCACCAAGATCCGCAGGGGCTGGGAGAA AGATGGGGCTGTCAAGTTCATGCTGCCCGGCGACCACACACAGGGGGAGAAAACAAGCCACGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207812 representing BC037435 Red=Cloning site Green=Tags(s) MSGEMDKPLISRRLVDSDGSLAEVPKEAPKVGILGSGDFARSLATRLVGSGFSVVVGSRNPKRTAGLFPS LAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLADQLAGKILVDVSNPTEKEHLQHRQSNAEYLASLFPAC TVVKAFNVISAWALQAGPRDGNRQVLICSDQPEAKRTISEMARAMGFTPLDMGSLASAREVEAIPLRLLP SWKVPTLLALGLFVCFYTYNFIRDVLQPYIRKDENKFYKMPLSVVNTTLPCVAYVLLSLVYLPGVLAAAL QLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFFAMLHALYSFCLPLRRSHRYDLVNLAVKQVLANKSRLWV EEEVWRMEIYLSLGVLALGMLSLLAVTSLPSIANSLNWKEFSFVQSTLGFVALILSTMHTLTYGWTRAFE ENHYKFYLPPTFTLTLLLPCVIILAKGLFLLPCLSRRLTKIRRGWEKDGAVKFMLPGDHTQGEKTSHV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037435
ORF Size	1466 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037435, AAH37435
RefSeq Size	1760 bp
RefSeq ORF	1466 bp
Locus ID	68428
Gene Summary	Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Also mediates reduction of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.[UniProtKB/Swiss-Prot Function]

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