Steap3 (BC037435) Mouse Tagged ORF Clone

CAT#: MG207812

  • TrueORF®

Steap3 (tGFP-tagged) - Mouse STEAP family member 3 (cDNA clone MGC:47007 IMAGE:2651584)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Tsap6, pHyde
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207812 representing BC037435
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGGGAGATGGACAAGCCGCTGATCAGCCGCCGCCTAGTGGACAGTGATGGCAGTCTGGCTGAGG
TCCCCAAGGAGGCCCCCAAAGTGGGCATCCTGGGCAGTGGGGATTTTGCCCGTTCCCTGGCCACACGCCT
GGTGGGCTCTGGCTTCAGTGTGGTGGTGGGGAGCCGTAACCCCAAACGCACGGCTGGCCTCTTCCCCTCC
TTAGCTCAAGTGACTTTCCAGGAGGAAGCCGTGAGCTCTCCAGAGGTCATCTTTGTGGCCGTGTTCCGGG
AGCACTATTCCTCTCTGTGCAGTCTCGCTGACCAGTTGGCTGGCAAGATCCTCGTGGATGTAAGCAACCC
CACGGAGAAGGAGCATCTTCAGCACCGCCAGTCTAACGCTGAGTACCTGGCCTCACTCTTTCCTGCGTGC
ACTGTGGTGAAGGCCTTCAACGTCATCTCTGCATGGGCCCTACAGGCTGGCCCAAGGGATGGGAACAGGC
AGGTGCTCATCTGCAGTGATCAGCCAGAAGCCAAGCGCACCATCTCAGAGATGGCACGCGCCATGGGTTT
CACACCCCTGGACATGGGATCCCTGGCCTCAGCGCGGGAGGTAGAAGCCATACCCCTGCGCCTCCTTCCA
TCCTGGAAGGTGCCCACCCTCCTGGCACTGGGGCTTTTTGTGTGCTTCTACACCTACAACTTCATCCGGG
ACGTTCTACAGCCATACATTCGGAAAGATGAGAACAAGTTCTACAAGATGCCCTTGTCTGTGGTCAACAC
CACACTACCCTGTGTGGCTTATGTGCTGCTGTCCCTAGTGTACCTGCCCGGTGTGCTGGCAGCTGCGCTT
CAGCTGCGGAGGGGGACCAAGTACCAGCGTTTCCCAGACTGGCTGGACCACTGGCTGCAGCATCGCAAGC
AGATCGGGCTGCTCAGCTTCTTCTTCGCGATGCTGCACGCTCTCTACAGCTTCTGCCTGCCGCTGCGCCG
CTCCCACCGCTATGACCTGGTCAATCTGGCTGTGAAGCAGGTCCTGGCCAACAAGAGCCGCCTCTGGGTT
GAGGAAGAAGTCTGGAGGATGGAGATATACCTGTCCCTGGGTGTGCTGGCCCTGGGCATGTTGTCGCTGC
TGGCTGTCACCTCGCTCCCGTCCATTGCTAATTCCCTCAACTGGAAGGAGTTCAGCTTTGTGCAGTCCAC
ACTGGGCTTCGTGGCCCTGATACTCAGCACAATGCACACACTCACCTACGGCTGGACCCGTGCCTTTGAG
GAAAACCACTACAAGTTCTACCTGCCGCCCACATTCACACTCACGCTGCTCCTGCCCTGTGTGATCATCC
TGGCCAAGGGCCTCTTCCTCCTGCCCTGCCTCAGCCGCAGACTCACCAAGATCCGCAGGGGCTGGGAGAA
AGATGGGGCTGTCAAGTTCATGCTGCCCGGCGACCACACACAGGGGGAGAAAACAAGCCACGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207812 representing BC037435
Red=Cloning site Green=Tags(s)

MSGEMDKPLISRRLVDSDGSLAEVPKEAPKVGILGSGDFARSLATRLVGSGFSVVVGSRNPKRTAGLFPS
LAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLADQLAGKILVDVSNPTEKEHLQHRQSNAEYLASLFPAC
TVVKAFNVISAWALQAGPRDGNRQVLICSDQPEAKRTISEMARAMGFTPLDMGSLASAREVEAIPLRLLP
SWKVPTLLALGLFVCFYTYNFIRDVLQPYIRKDENKFYKMPLSVVNTTLPCVAYVLLSLVYLPGVLAAAL
QLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFFAMLHALYSFCLPLRRSHRYDLVNLAVKQVLANKSRLWV
EEEVWRMEIYLSLGVLALGMLSLLAVTSLPSIANSLNWKEFSFVQSTLGFVALILSTMHTLTYGWTRAFE
ENHYKFYLPPTFTLTLLLPCVIILAKGLFLLPCLSRRLTKIRRGWEKDGAVKFMLPGDHTQGEKTSHV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037435
ORF Size 1466 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037435, AAH37435
RefSeq Size 1760 bp
RefSeq ORF 1466 bp
Locus ID 68428
Gene Summary Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Also mediates reduction of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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