Ccdc67 (BC096547) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4933401K09Rik; Deup1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207782 representing BC096547 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAAATCGACATCATGGTAAGCAACAAGAAGCTAGACTGGGAAAGGAAGATGCGGGCTTTAGAGA CACGGTTAGACCTTCGGGATCAAGAGTTGGCCAACGCACAGACTTGCTTGGACCAGAAAGGCCAGGAGGT GGGGTTGCTTCGGCAGAAGCTGGACAGCCTAGAGAAATGTAATCTAGTAATGACTCAGAACTATGAGGGG CAGCTACAGACCCTAAAAGCTCAATTTTCTAAGCTAACCAGTAACTTCGAAAAGCTGAGATTACACCAAA TGAAGCAAAACCAAATTCACCGAAAGGAGTCCTCCAGCAAAGAAGAACTGCCCTTTGAGCTGAGCAGTCT GAACCAGAAACTAGAGGAGTTCCGAGCAAAGTCGAGGGAGTGGGACAAGCAGGAGGTCCTCTACCAGACC CACCTGGTGTCCCTGGATGCTCAGCAGAAGTTGCTCTCTGAAAAGTGCAGCCAGTTTCAGAAACAGGCAC AAAATTACCAAACTCAGCTAAACGGGAAAAAGCAGTGCGCAGAGGACAGCAGCTCTGAGATCCCTCGCCT GGTGTGTGAGTCGGATCCTGGCTGTGAAGCCACTCAAAGAGATGAGTTCATTATCGAGAAGTTAAAGTCG GCCGTGAGTGAAATCGCGCTGAGCAGGAACAAGTTACAAGATGAAAACCAGAAGCTCCTGCAGGAACTGA AGATGTACCAGAGGCAGTGCCAGGCCATGGAAGCAGGACTGTCAGAGGTGAAGAGTGAGTTACAGTCGCG AGACGATCTCCTGAGGATTATAGAAATGGAAAGACTGCACTTGCATAGAGAACTGCTGAGAATGGGTGAA GTGCAGACTGCTCAAGATAACAGGAAAAGAGTTGAGTCATCATATTCCCCTTCTCCTAAAGAAGCAGAAA GGAAAAGGAAGGAGTTGTTTCCAATGGTCTCAGATCAACCCAATCATGAAAAGGAATTAAGCAAGGCTGT GGACTTAAGTAACAAGAAGCATTCACAGTGCACATCAATTAACAAGCTTGAATATGAGAACGAAAGACTC CGAAGCGATCTCGCCAAGCTCCATGGCAATGGAAAAGCAGCGTGGCCCAATCAGAGCAGCTATGGTGAAG CAGGGGCATACGTCTTCCAGAGCCAGCTGAAAACGGAGACCAGTGGAGACAGAATTAGCCAAGACTGCGA GCTGAACAGAAGCCCCACACCCTTGTCGCCTTTGCCATTTCAGACCAAAGAAATGGCAAGCCCATTGGTT GGTGACAATGAAGTCCTCGCTCTGTCTCCACCAGATATATCCTTCCGGGCTTCCCTAGCTGCACAGCACT TCCTGATGGAGGAAGAGAGGCGGGCCAAGGAGCTTGAGAAGCTGCTCAACACACACATCGATGAGCTGCA GAGACACACCGAGTTCACGCTTAACAAATACACCAAACTCAAGCAGAGTAGACACATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207782 representing BC096547 Red=Cloning site Green=Tags(s) MEQIDIMVSNKKLDWERKMRALETRLDLRDQELANAQTCLDQKGQEVGLLRQKLDSLEKCNLVMTQNYEG QLQTLKAQFSKLTSNFEKLRLHQMKQNQIHRKESSSKEELPFELSSLNQKLEEFRAKSREWDKQEVLYQT HLVSLDAQQKLLSEKCSQFQKQAQNYQTQLNGKKQCAEDSSSEIPRLVCESDPGCEATQRDEFIIEKLKS AVSEIALSRNKLQDENQKLLQELKMYQRQCQAMEAGLSEVKSELQSRDDLLRIIEMERLHLHRELLRMGE VQTAQDNRKRVESSYSPSPKEAERKRKELFPMVSDQPNHEKELSKAVDLSNKKHSQCTSINKLEYENERL RSDLAKLHGNGKAAWPNQSSYGEAGAYVFQSQLKTETSGDRISQDCELNRSPTPLSPLPFQTKEMASPLV GDNEVLALSPPDISFRASLAAQHFLMEEERRAKELEKLLNTHIDELQRHTEFTLNKYTKLKQSRHI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC096547
ORF Size	1460 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC096547, AAH96547
RefSeq Size	2942 bp
RefSeq ORF	1460 bp
Locus ID	234964
Gene Summary	Key structural component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells. Deuterosome-mediated centriole amplification occurs in terminally differentiated multiciliated cells and can generate more than 100 centrioles. Probably sufficient for the specification and formation of the deuterosome inner core. Interacts with CEP152 and recruits PLK4 to activate centriole biogenesis.[UniProtKB/Swiss-Prot Function]

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