Papd4 (BC016629) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	8030446C20Rik; AI649009; GLD-2; Gld2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207763 representing BC016629 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCAAACTCAATTTTGGGTCGTCCACCCTTCACTCCAACCCACCAACAGCATAATAACTTCTTTG CTCTCTCACCAACTCTTTATTCACACCAGCAACTTATAGATGCACAATTCAACTTTCAAAATGTAGACTT GTCTAGAGCTGTGTCCTTACAGCCTCTGACATATGGAACTGTCAGTCCAATACAGACCTCAACATCTCCA TTATTTCGAGGAAGGAAGAGAATAAGCGATGAAAAAGCTTTTCCTCTTGATGGTAAACGGCAGCGTTTCC ATTCACCCCACCAAGAGCCAACTATAATCAACCAGCTAGTGCCTTTATCAGGTGACAGAAGATATTCCAT GCCACCGTTGTTTCACACGCACTATATACCAGATATAGTCAGATGTGTTCCACCTCTTCGAGAAATTCCA CTGTTAGAACCTCGAGAAATCACACTGCCTGAGGCCAAAGATAAGTTGAGTCAGCAGATACTAGAGTTGT TTGAAACTTGTCAGCAGCAAGCAAGTGACTTGAAGAAGAAAGAACTCTGTAGAGCACAGCTGCAGAGAGA GATCCAGCTGCTCTTCCCACAAAGCAGACTTTTTCTCGTTGGATCATCTTTGAATGGATTTGGTGCCCGA AGCAGTGATGGCGATTTATGCTTAGTTGTTAAAGAAGAACCATGTTTTTTCCAGGTAAATCAGAAGACTG AAGCACGACATATACTCACCTTAGTCCATAAGCACTTCTGTACTAGACTGTCTGGCTATATTGAGAGACC TCAGCTGATTCGAGCAAAAGTGCCAATTGTGAAGTTCAGGGATAAAGTCAGTTGTGTGGAATTCGACTTA AATGTAAACAATACCGTTGGAATTAGAAATACATTCCTTCTCAGAACCTATGCATACCTTGAAAATCGAG TTCGTCCATTAGTGCTGGTGATTAAGAAGTGGGCAAGTCACCATGACATCAATGATGCCAGTCGTGGCAC ATTAAGCAGCTATAGTCTTGTATTGATGGTTTTGCACTATTTACAAACCCTACCTGAACCCATCCTTCCA TCCCTCCAAAAAATTTACCCAGAATCTTTTAGTACTTCTGTCCAGCTGCACCTTGTTCATCATGCCCCAT GTAATGTCCCTCCTTACCTCTCAAAGAATGAATCAAGTCTTGGGGACCTCTTACTGGGCTTTCTGAAATA TTATGCTACAGAATTTGACTGGAACACTCAGATGATTTCAGTTCGTGAAGCCAAAGCCATCCCAAGGCCT GATGATATGGAATGGAGAAATAAATACATCTGTGTTGAAGAACCTTTTGATGGAACTAATACCGCCAGAG CTGTGCATGAGAAGCAGAAGTTTGATATGATCAAGGATCAATTTTTAAAGTCATGGCAGAGATTGAAAAA CAAGAGAGATCTGAACAGTGTGCTGCCTTTGAGAGCTGCTACCCTGAAAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207763 representing BC016629 Red=Cloning site Green=Tags(s) MFPNSILGRPPFTPTHQQHNNFFALSPTLYSHQQLIDAQFNFQNVDLSRAVSLQPLTYGTVSPIQTSTSP LFRGRKRISDEKAFPLDGKRQRFHSPHQEPTIINQLVPLSGDRRYSMPPLFHTHYIPDIVRCVPPLREIP LLEPREITLPEAKDKLSQQILELFETCQQQASDLKKKELCRAQLQREIQLLFPQSRLFLVGSSLNGFGAR SSDGDLCLVVKEEPCFFQVNQKTEARHILTLVHKHFCTRLSGYIERPQLIRAKVPIVKFRDKVSCVEFDL NVNNTVGIRNTFLLRTYAYLENRVRPLVLVIKKWASHHDINDASRGTLSSYSLVLMVLHYLQTLPEPILP SLQKIYPESFSTSVQLHLVHHAPCNVPPYLSKNESSLGDLLLGFLKYYATEFDWNTQMISVREAKAIPRP DDMEWRNKYICVEEPFDGTNTARAVHEKQKFDMIKDQFLKSWQRLKNKRDLNSVLPLRAATLKR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016629
ORF Size	1454 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016629, AAH16629
RefSeq Size	1897 bp
RefSeq ORF	1454 bp
Locus ID	100715
Gene Summary	Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.