Chst2 (BC051963) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Chts2, GlcNAc6ST, Gn6st, GST-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207740 representing BC051963 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTATTTCGCAGGAAGGCGCTGGTGCTGTGCGCGGGCTATGCACTGCTACTGGTGCTCACGATGC TCAACCTCTTGGACTACAAGTGGCATAAAGAGCCGCTGCAGCAGTGCAACCCCGACGGGCCTCTGGGTGC CGCGGTAGGGGCGGCCGGGGCCGGCTGGGGACGGCCGGGGTCGCCTCCTGCAGCGCCACCCCGCGCTCAC TCTCGCATGGACCCCCGCACCCCGTACCGCCCTCCTGCCGCGGGCGTGGGGGCAGTTCCCGCAGCCGCGG CTGGGAGTGCAGGAGCTGCGGCCTCTCTGGGCAATGCTACTCGAGGCACCAGGGGTGGAGGGGACAAGCG GCAGTTGGTGTATGTGTTCACCACGTGGCGCTCGGGCTCGTCCTTCTTCGGTGAGCTCTTCAACCAGAAC CCTGAGGTGTTCTTCCTCTATGAGCCTGTGTGGCACGTGTGGCAAAAACTGTACCCCGGGGACGCCGTTT CCCTGCAGGGGGCAGCGCGGGACATGCTGAGCGCTCTCTACCGCTGCGATCTTTCGGTTTTCCAGCTGTA TAGCCCCGCAGGCAGTGGGGGGCGCAACCTCACCACTCTGGGCATCTTTGGGGCAGCCACTAACAAGGTG GTATGCTCCTCGCCACTCTGTCCTGCCTACCGCAAGGAGGTCGTCGGACTGGTGGACGACCGCGTGTGCA AAAAGTGCCCACCTCAACGCCTGGCACGCTTCGAGGAGGAGTGTCGCAAGTACCGCACGCTGGTTATCAA GGGCGTGCGGGTCTTCGATGTGGCTGTGTTGGCGCCGCTGCTTAAAGATCCAGCCTTGGACCTCAAGGTC ATCCACCTAGTACGTGATCCTCGTGCTGTTGCCAGCTCCCGCATCCGCTCGCGTCACGGCCTCATCCGGG AAAGCCTACAGGTGGTGCGAAGCCGGGATCCAAGAGCCCACCGCATGCCCTTCCTGGAGGCTGCTGGCCA CAAGCTTGGTGCCAAGAAGGAGGGTATGGGTGGCCCAGCAGACTACCACGCTCTGGGTGCAATGGAGGTC ATCTGCAACAGTATGGCCAAGACGCTGCAAACAGCCCTGCAGCCTCCTGACTGGCTGCAGGGACACTACT TGGTGGTGAGGTACGAGGATCTGGTGGGAGACCCCGTTAAGACCCTACGGAGGGTATATGACTTTGTGGG GCTGCTGGTGAGTCCCGAAATGGAGCAGTTTGCCCTGAACATGACCAGTGGTTCGGGCTCCTCCTCCAAG CCTTTCGTGGTGTCAGCTCGCAATGCCACTCAGGCCGCCAATGCCTGGCGGACCGCGCTCACCTTCCAGC AGATCAAACAGGTGGAGGAGTTTTGCTACCAGCCCATGGCCGTGCTGGGCTATGAGCGGGTTAACAGTCC TGAGGAGGTCAAAGACCTCAGCAAGACCTTGCTCAGGAAGCCCCGGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207740 representing BC051963 Red=Cloning site Green=Tags(s) MKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAVGAAGAGWGRPGSPPAAPPRAH SRMDPRTPYRPPAAGVGAVPAAAAGSAGAAASLGNATRGTRGGGDKRQLVYVFTTWRSGSSFFGELFNQN PEVFFLYEPVWHVWQKLYPGDAVSLQGAARDMLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKV VCSSPLCPAYRKEVVGLVDDRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLKDPALDLKV IHLVRDPRAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGMGGPADYHALGAMEV ICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSK PFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC051963
ORF Size	1451 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051963, AAH51963
RefSeq Size	2501 bp
RefSeq ORF	1451 bp
Locus ID	54371
Gene Summary	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.[UniProtKB/Swiss-Prot Function]

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