Wars (BC003450) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TrpRS; WRS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207719 representing BC003450 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACATGCCCAGTGGCGAGTCCTGCACCTCTCCACTGGAGCTGTTCAACAGCATAGCTACTCAAG GGGAGCTCGTGAGGTCCCTCAAAGCTGGAAATGCACCAAAAGATGAGATTGATTCTGCAGTAAAGATGTT GTTATCATTAAAAGTGAGCTATAAGGCCGCCATGGGGGAGGAGTACAAGGCTGGCTGCCCTCCTGGGAAC CCAACAGCTGGGAGAAATTGTGACTCTGATGCCACAAAAGCCAGCGAGGATTTTGTGGATCCATGGACAG TGCGGACAAGCAGTGCAAAAGGCATCGACTATGACAAGCTCATTGTTCAGTTTGGAAGCAGCAAAATTGA CAAAGAGCTGATAAACCGAATAGAGCGAGCCACTGGCCAAAGACCGCACCGCTTCCTGCGCCGGGGCATC TTCTTCTCCCACCGAGATATGAATCAAATTCTGGATGCCTATGAAAATAAGAAGCCATTCTACCTGTACA CAGGCAGGGGCCCCTCCTCCGAAGCAATGCATCTGGGTCATCTCGTCCCGTTTATTTTCACAAAGTGGCT ACAGGATGTGTTCAATGTGCCTTTGGTCATCCAGATGTCTGATGACGAGAAGTACCTGTGGAAGGACTTG ACTCTGGAGCAGGCATATAGCTACACCGTAGAAAATGCAAAGGACATCATTGCCTGTGGCTTCGACATCA ACAAAACTTTCATCTTCTCTGATCTTGAGTATATGGGGCAGAGCCCGGGCTTCTACAGGAACGTGGTGAA GATTCAGAAGCACGTCACCTTCAACCAAGTGAAAGGCATTTTCGGCTTTACGGACAGTGACTGCATCGGG AAGATCAGTTTTCCTGCTGTCCAGGCCGCGCCATCGTTCAGCAACTCTTTCCCAAAGATCTTCCGAGACA GGACAGATATCCAGTGCCTCATCCCGTGTGCCATTGACCAGGATCCGTACTTCAGAATGACAAGGGACGT GGCCCCCAGGATCGGCCATCCTAAACCTGCCCTGCTGCACTCGACCTTCTTCCCTGCCCTGCAGGGTGCT CAGACCAAGATGAGTGCCAGTGACCCCAACTCCTCCATCTTCCTCACTGACACGGCCAAGCAGATCAAGA GCAAGGTCAACAAGCATGCATTTTCTGGAGGAAGAGACACTGTGGAGGAGCACAGGCAGTTTGGGGGCAA CTGTGAAGTGGATGTATCCTTCATGTATCTGACCTTCTTCCTTGAAGATGACGACAGGCTGGAGCAGATC AGAAAGGATTACACCAGCGGAGCCATGCTCACTGGGGAGCTCAAGAAGACGCTCATAGACGTCCTTCAGC CCCTGATCGCAGAGCACCAGGCCCGACGCAAGGCGGTCACCGAGGAGACCGTGAAGGAGTTCATGACGCC CAGGCAGCTGTCCTTCCACTTCCAGTGCTTCTGCTTTGACACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207719 representing BC003450 Red=Cloning site Green=Tags(s) MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKVSYKAAMGEEYKAGCPPGN PTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGI FFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDL TLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIG KISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGA QTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQI RKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQCFCFDT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC003450
ORF Size	1443 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003450, AAH03450
RefSeq Size	1838 bp
RefSeq ORF	1445 bp
Locus ID	22375
Gene Summary	T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).[UniProtKB/Swiss-Prot Function]

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