Fbxo3 (BC058253) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1200002G09Rik; 1700026K02Rik; AI046358; Fba
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207690 representing BC058253 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGTAGAGGCCGAGACGGGGCTGCTGACCCTGGAGTCGCTGCCCACCGACCCTCTGCTGCTCA TCTTATCCTTCGTGGACTACAGGGACCTAATCAATTGTTGCTATGTTAGTCGAAGACTTAGCCAGCTTTC AACTCATGATCCACTGTGGAGAAGACATTGCAAAAAATACTGGCTGATATCTGAGGAAGAAAAAGCCGGG AAGAGTCAGTGCTGGAGATCCCTCTTCATAGAGACCTACTCAGACGTGGGGAGGTACATCGACCATTACG CCGCCATTAAAAAGGCCTGGCGTGACCTCAAGAAGTACTTGGAGCCCAGGTGTCCTCGGATGGTTTTATC TCTGAAAGAGGGCGCACGTGAGGAAGACCTGGATGCTGTCGAAGCTCAGATCGGTTGCAAGCTCCCGGAT GATTATCGCTGTTCATACCGGATCCACAACGGGCAAAAGTTAGTGGTGCCGGGGTTGCTGGGAAGTATGG CACTGTCCAATCACTACCGCTCTGAAGATCTACTAGATGTTGATACTGCTGCAGGAGGATTTCAGCAGAG ACAGGGACTGAAATACTGTCTCCCTTTAACCTTTTGCATACACACCGGCTTGAGTCAGTACATAGCTGTG GAGGCTGCAGAGGGGCGGAATAAGAACGAAGTGTTCTACCAATGTCCAGACCAAATGGCTCGGAATCCTG CTGCTATTGACATGTTTATCATAGGTGCTACTTTTACCGACTGGTTTACATCCTATGTCAACAATGTTGT ATCAGGTGGTTTCCCCATCATCAGAGACCAGATTTTCAGATATATTCATGATCCAGAATGTGTGGCAACA ACTGGAGATATTACAGTTTCAGTGTCCACATCGTTTCTGCCAGAACTCAGCTCTGTGCATCCGCCACACT ATTTCTTCACATACCGAATCAGGATTGAAATGTCAAGGGATGCTCTTCCTGAGAAGGCCTGTCAGTTGGA CAGTCGCTACTGGAGGATAACAAACGCTAAAGGGGATGTGGAAGAAGTCCAGGGACCTGGAGTAGTCGGT GAATTTCCAATTATTAGCCCAGGTCGGATATATGAATACACAAGCTGTACCACATTTTCTACAACATCAG GCTACATGGAAGGGTATTACACCTTCCATTTTCTGTACTTTAAGGACAAGGTCTTTAATGTTGCCATCCC CCGATTCCACATGGCATGTCCAACATTCAGGGTGTCTATAGCTCGATTGGAAATGGGTCCTGATGAATAT GAAGAGATGGAGGAGGAAGCTGAGGAGGAGGAGGAGGAAGAAAATGATGACTCTGCAGACATGGATGAAT CCGATGAGTCAGATGCGGATGAGAATGAGTCAGATGAGGGTGAAGGGGAGGCGAGACGGAGGAGGGTCTT TGATGTTCCCATTCGCAGACGCCGCTGCTCTCGTCTGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207690 representing BC058253 Red=Cloning site Green=Tags(s) MAAVEAETGLLTLESLPTDPLLLILSFVDYRDLINCCYVSRRLSQLSTHDPLWRRHCKKYWLISEEEKAG KSQCWRSLFIETYSDVGRYIDHYAAIKKAWRDLKKYLEPRCPRMVLSLKEGAREEDLDAVEAQIGCKLPD DYRCSYRIHNGQKLVVPGLLGSMALSNHYRSEDLLDVDTAAGGFQQRQGLKYCLPLTFCIHTGLSQYIAV EAAEGRNKNEVFYQCPDQMARNPAAIDMFIIGATFTDWFTSYVNNVVSGGFPIIRDQIFRYIHDPECVAT TGDITVSVSTSFLPELSSVHPPHYFFTYRIRIEMSRDALPEKACQLDSRYWRITNAKGDVEEVQGPGVVG EFPIISPGRIYEYTSCTTFSTTSGYMEGYYTFHFLYFKDKVFNVAIPRFHMACPTFRVSIARLEMGPDEY EEMEEEAEEEEEEENDDSADMDESDESDADENESDEGEGEARRRRVFDVPIRRRRCSRLF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058253
ORF Size	1442 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058253, AAH58253
RefSeq Size	2368 bp
RefSeq ORF	1442 bp
Locus ID	57443
Gene Summary	Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (By similarity).[UniProtKB/Swiss-Prot Function]

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