Metap2 (BC002213) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Amp2, Mnpep, p67, p67eIF2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207657 representing BC002213 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTGGAGCAGGCAGCGTCCTTCGGGGGACACCTGAATGGCGACCTGGATCCAGACGACAGGG AAGAGGGAACCTCCAGCACGGCCGAGGAAGCCGCCAAGAAGAAAAGACGGAAGAAGAAGAAGGGCAAAGG GGCTGTGTCAGCAGTGCAACAAGAACTTGATAAAGAATCCGGAGCCTTGGTGGATGAAGTAGCAAAACAG CTGGAGAGCCAAGCACTGGAGGAGAAGGAGAGAGATGACGACGATGAAGATGGAGATGGTGATGCTGATG GTGCAACTGGGAAGAAGAAGAAAAAGAAGAAGAAGAAGAGAGGACCAAAAGTTCAAACAGACCCTCCCTC AGTTCCAATATGTGACCTGTATCCTAATGGTGTATTTCCCAAAGGACAAGAGTGTGAATACCCACCCACA CAAGATGGGCGGACAGCTGCTTGGAGAACCACAAGTGAGGAAAAAAAGGCCCTAGACCAGGCCAGTGAGG AGATCTGGAACGACTTCCGAGAAGCTGCGGAGGCACATCGGCAAGTTAGGAAATATGTCATGAGCTGGAT CAAGCCTGGGATGACGATGATAGAAATCTGTGAGAAGTTGGAAGACTGTTCCCGAAAGCTAATAAAGGAA AATGGGTTAAATGCAGGCCTGGCGTTCCCCACTGGGTGTTCTCTCAACAACTGTGCTGCCCATTACACTC CCAATGCTGGTGACACGACAGTCTTGCAGTATGATGACATCTGTAAGATAGACTTTGGAACACATATAAG TGGTAGAATAATCGATTGTGCTTTTACTGTTACTTTTAATCCCAAATATGACATACTATTAACAGCTGTA AAGGATGCCACTAATACTGGAATAAAGTGTGCTGGGATTGACGTTCGTCTCTGCGATGTCGGTGAGGCCA TTCAAGAAGTTATGGAATCCTATGAAGTAGAAATAGATGGGAAGACATACCAAGTGAAACCCATACGTAA CTTAAATGGACATTCAATTGGGCCATATAGAATTCACGCTGGAAAAACGGTGCCCATTGTGAAAGGAGGG GAAGCTACAAGAATGGAGGAAGGAGAGGTGTATGCCATTGAGACCTTTGGGAGCACGGGGAAGGGCGTGG TTCATGACGACATGGAATGTTCACACTACATGAAAAATTTTGATGTGGGGCACGTGCCAATAAGGCTTCC AAGAACAAAACACTTGTTAAATGTCATCAACGAAAACTTCGGTACCCTTGCCTTCTGCCGAAGGTGGCTG GATCGCTTGGGAGAAAGTAAATACTTAATGGCTCTGAAGAATCTGTGTGACTTGGGCATTGTAGATCCAT ACCCACCACTGTGTGACATTAAAGGGTCATATACAGCACAGTTTGAACACACTATACTGTTGCGTCCAAC CTGTAAAGAAGTTGTCAGCAGAGGAGATGACTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207657 representing BC002213 Red=Cloning site Green=Tags(s) MAGVEQAASFGGHLNGDLDPDDREEGTSSTAEEAAKKKRRKKKKGKGAVSAVQQELDKESGALVDEVAKQ LESQALEEKERDDDDEDGDGDADGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPT QDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKE NGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDILLTAV KDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGPYRIHAGKTVPIVKGG EATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWL DRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002213
ORF Size	1434 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002213, AAH02213
RefSeq Size	1981 bp
RefSeq ORF	1436 bp
Locus ID	56307
Gene Summary	Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).[UniProtKB/Swiss-Prot Function]

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