Rtn2 (NM_013648) Mouse Tagged ORF Clone

CAT#: MG207524

  • TrueORF®

Rtn2 (tGFP-tagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B

ORF Plasmid: DDK tGFP



  "NM_013648" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,560.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MMS10-P; Ms10p; Nspl1; NSPLI
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207524 representing NM_013648
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGGTCCTGCCGGTCTTCGCCCACTGCAAAGAAGCTCCATCTACAGCCTCTTCTACCCCCGATT
CCACGGAAGGAGGGAATGACGACTCAGATTTTCGGGAGCTGCACACAGCCCGGGAATTCTCAGAGGATGA
AGAGGAGGAGACCACATCGCAGGACTGGGGCACCCCCAGGGAGCTGACCTTTTCCTACATTGCCTTCGAC
GGCGTGGTGGGCTCTGGGGGCCGTAGGGACTCAGTTGTTCGTCGTCCCCGGCCCCAGGGACGTTCAGTCT
CAGAACCACGGGACCCACCCCAACAGTCCGGCCTGGGTGACAGCTTGGAAAGCATCCCTAGCCTGAGCCA
GTCCCCAGAGCCAGGGCGCCGCGGTGACCCTGACCCCGTGCCTCCAGCTGAGCGCCCGCTGGAGGAACTG
AGGCTGCGGCTGGACCAGCTTGGCTGGGTTGTCCGGAGTGCGGGATCTGGGGAGGATTCGGCCACCAGCA
GCTCCACCCCTCTGGAAAATGAGGAGCCTGATGGATTGGAGGCCAGCGAGGCTGGAGAAGAGACGAATCT
GGAACTCCGACTTGCTCAGTCTCTACATTTGCAACTTGAGGTCCTGACTCCTCAACTTAGCCCTAGCTCC
GGGACCCCCCAGGCGCATACTCCATCCCCACAAAGATCTCAAGATTCGAACTCCGGTCCTGATGATGAGC
CTCTGCTGAATGTGGTAGAAGAGCATTGGCGGCTGCTGGAGCAGGAGCCAATCACGGCCCAGTGCCTCGA
TAGCACAGACCAATCAGAATTCATGTTGGAGCCGCTCCTTCTAGTGGCAGACCTGTTGTACTGGAAGGAC
ACCAGGACGTCGGGAGCGGTCTTCACGGGCCTCATGGCCTCTCTCCTCTGCCTCCTGCACTTTAGCATCG
TGTCCGTGGCTGCGCACCTGGCCCTGTTGGGTCTCTGCGCCACCATCTCTCTCAGGGTTTACCGCAAAGT
GCTGCAGGCCGTGCACCGGGGCGACGGCACCAACCCTTTCCAGGCCTACCTGGACATGGACCTGACCCTG
ACTCGGGAGCAGACAGAACGTTTGTCCCAGCAGATCGCCTCCCACGTGGTCTCCACAGCCACACAGCTAC
GGCATTTCTTCCTAGTGGAAGACCTGGTGGATTCTCTCAAGCTGGCTCTTCTCTTCTACATCTTGACCTT
TGTGGGAGCCATCTTCAACGGCTTGACTCTTGTCATCCTAGGAGTGGTTGCTTTGTTTACCGTCCCCCTT
CTGTACAGACAACATCAGGCCCAGATTGACCAGTATGTGGGGTTGGTGACCAATCAGTTGAGCCACATCA
AAGCTAAGATCCGAGCTAAGATCCCGGGGACCGGAACCCTTGCCCCAACAGCATCAGTCTCCGGATCCAA
AGCCAAAGCCGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207524 representing NM_013648
Red=Cloning site Green=Tags(s)

MGQVLPVFAHCKEAPSTASSTPDSTEGGNDDSDFRELHTAREFSEDEEEETTSQDWGTPRELTFSYIAFD
GVVGSGGRRDSVVRRPRPQGRSVSEPRDPPQQSGLGDSLESIPSLSQSPEPGRRGDPDPVPPAERPLEEL
RLRLDQLGWVVRSAGSGEDSATSSSTPLENEEPDGLEASEAGEETNLELRLAQSLHLQLEVLTPQLSPSS
GTPQAHTPSPQRSQDSNSGPDDEPLLNVVEEHWRLLEQEPITAQCLDSTDQSEFMLEPLLLVADLLYWKD
TRTSGAVFTGLMASLLCLLHFSIVSVAAHLALLGLCATISLRVYRKVLQAVHRGDGTNPFQAYLDMDLTL
TREQTERLSQQIASHVVSTATQLRHFFLVEDLVDSLKLALLFYILTFVGAIFNGLTLVILGVVALFTVPL
LYRQHQAQIDQYVGLVTNQLSHIKAKIRAKIPGTGTLAPTASVSGSKAKAE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013648
ORF Size 1413 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013648.6
RefSeq Size 1947 bp
RefSeq ORF 1416 bp
Locus ID 20167
UniProt ID O70622
Gene Summary Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity (By similarity). Enhances trafficking of the glutamate transporter SLC1A1/EAAC1 from the endoplasmic reticulum to the cell surface (By similarity). Plays a role in the translocation of SLC2A4/GLUT4 from intracellular membranes to the cell membrane which facilitates the uptake of glucose into the cell (PubMed:19720795).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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