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Trim16 (BC052821) Mouse Tagged ORF Clone
CAT＃: MG207510
- TrueORF®
Trim16 (tGFP-tagged) - Mouse tripartite motif protein 16 (cDNA clone MGC:60760 IMAGE:30057830)
ORF Plasmid: DDK
"BC052821" in other vectors (4)
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CNY 5,256.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9130006M08Rik; AI482483; EBBP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207510 representing BC052821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTACTGTGAGGAGCACCTGCGGCCACACCAGGAGAACAGCAAACTGCACAGCCACCAGCTAA CCGAACCCGCAAAGGACCAGGATCTACGCACCTGCCCTGCCCACCACAGCCCTTTGGTTTCCTTCTGCCA CACGCACCAGCAGTGTATCTGCCAGGAATGCGGCGAGGGTGAACACAGAGGCGACAGTACTGTCTCCTTG GATGCAGCCCGCAGGAACAAAGAGGTGGACCTTCGGTGTATGCAGTTGGACCTGGAGCAGAAGCTTAAGT TGAATGAAAATGCCATTGCCAGGCTCCAAGCTAACCATAAGTCTGTTTTGGTGTCGGTGTCAGAGGTGAA GGTGGTAGCTGAAGAGAAGTTTGGGGAACTCCTCGCAGCTGTACGGAAGGCCCAGGCAGATGTGATGGTC TTCCTAGAGGAGAAAGAACAGGCTGCACTGAATCAAGTCAACAGCATCAAAACCCATCTGGAGCACAGGA GCCTCGAGATGGAGAAAAGCAAGCAGGAGCTGGAGAGGCTGGCCGCCATCAGCAACACTGTGCTGTTCCT GGAGGAGTACTGCAAGCTCAAGAAGACAGAGGACACTGCCTCCCCTAGCATTTACATAGGGCTGAAGGAC AAACTCTCAGGCATCCGCAAGGTCATCACAGATTCAACTCTGAACTTGATCCAATTGCTAGAAAGCTATA AGAAAAAGCTCCAGGAATTTTCCAGGGAAGAGGAATATGACATCAGAACTCAAGTGTCTGCCATTGTCCA GCGCAAGTATAGGACCTCGAAACCTGAGCCCAGAACCCGGGATGAGTTTCTTCAATATGCATGTGACATC ACGTTTGACCCAGACACAGCACACAGGTACCTTCGCCTGCAGGAGGACAACCGCAAGGTCACCAACACCA CGCCCTGGGAACACCCCTACCCGGACCTCCCCAGTAGATTTCTGCACTGGCGGCAGGTGCTGTCCCAACA GAGCCTGTACCTGCACAGATATTATTTTGAGGTGGAGCTCTCTGGAGGTGGCACCTATGTTGGCCTGACC TGCAAAGGCATTGACCGTAAGGGGGAGGAACGGAACAGCTGCATTTCTGGAAATAGCTTCTCCTGGAGCA TCCATTGGAATGGGAAGGAGTTCACAGCCTGGCACAGTGACACAGAGACCCCGCTCAAGGCCAGCCCCTT CAGAAGGCTCGGTATCTATGTAAACTTCCCAGGAGGAATCCTTTCCTTCTATGGTGTAGAGTATGATGCT ATGACTCTGATTCACAAGTTTGAATGCAAGTTCTCAGAGCCGGTCTATGCTGCTTTCTGGCTGTCTAAGA AAGAAGACGCCATCCGGATTGTGGATCTGGGAGAGGAGCCCGAGAAGCCAGCTGGGTCCTCTGTGGAGGC TGCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207510 representing BC052821
Red=Cloning site Green=Tags(s) MVNYCEEHLRPHQENSKLHSHQLTEPAKDQDLRTCPAHHSPLVSFCHTHQQCICQECGEGEHRGDSTVSL DAARRNKEVDLRCMQLDLEQKLKLNENAIARLQANHKSVLVSVSEVKVVAEEKFGELLAAVRKAQADVMV FLEEKEQAALNQVNSIKTHLEHRSLEMEKSKQELERLAAISNTVLFLEEYCKLKKTEDTASPSIYIGLKD KLSGIRKVITDSTLNLIQLLESYKKKLQEFSREEEYDIRTQVSAIVQRKYRTSKPEPRTRDEFLQYACDI TFDPDTAHRYLRLQEDNRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVELSGGGTYVGLT CKGIDRKGEERNSCISGNSFSWSIHWNGKEFTAWHSDTETPLKASPFRRLGIYVNFPGGILSFYGVEYDA MTLIHKFECKFSEPVYAAFWLSKKEDAIRIVDLGEEPEKPAGSSVEAAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052821 |
| ORF Size | 1407 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052821, AAH52821 |
| RefSeq Size | 3644 bp |
| RefSeq ORF | 1409 bp |
| Locus ID | 94092 |
| Gene Summary | E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205970 | Trim16 (untagged) - Mouse tripartite motif protein 16 (cDNA clone MGC:60760 IMAGE:30057830), (10ug) |
CNY 3,656.00 |
|
| MR207510 | Trim16 (Myc-DDK-tagged) - Mouse tripartite motif protein 16 (cDNA clone MGC:60760 IMAGE:30057830) |
CNY 4,660.00 |
|
| MR207510L3 | Lenti ORF clone of Trim16 (Myc-DDK-tagged) - Mouse tripartite motif protein 16 (cDNA clone MGC:60760 IMAGE:30057830) |
CNY 6,560.00 |
|
| MR207510L4 | Lenti ORF clone of Trim16 (mGFP-tagged) - Mouse tripartite motif protein 16 (cDNA clone MGC:60760 IMAGE:30057830) |
CNY 6,560.00 |
