Ero1lb (BC058721) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC77955, ero1-beta
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207470 representing BC058721 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCCGGGGTTCCGCCGGGCCGTTACTGGGCAGGGGGCGGCGGCCGCGGTGCAACTGCTTGTCACCC TGAGCTTCCTCTCAAGTCTGGTCAAGACTCAGGTGACTGGAGTTCTGGATGATTGCTTATGTGACATTGA CAGCATTGATAAATTCAACACCTACAAAATCTTTCCCAAAATAAAGAAGTTACAAGAACGAGACTATTTT CGTTATTACAAGGTTAATCTGAAACGACCATGTCCTTTCTGGGCAGAAGATGGCCACTGCTCAATAAAAG ACTGTCATGTGGAGCCCTGTCCAGAAAGTAAAATTCCAGTTGGAATTAAAGCCGGGCGTTCAAATAAGTA CTCGCAAGCAGCAAACAGCACCAAAGAACTGGATGACTGTGAGCAGGCTAACAAACTGGGCGCCATCAAC AGCACGCTAAGTAACGAAAGCAAAGAAGCGTTCATTGACTGGGCGAGATATGATGATTCGCAGGACCACT TTTGTGAACTTGATGATGAGCGGTCTCCTGCTGCACAGTATGTGGACCTGCTGCTGAACCCGGAACGGTA CACTGGCTACAAGGGCTCCTCAGCATGGAGGGTGTGGAACAGCATCTATGAAGAAAACTGCTTCAAGCCT CGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGATGGAGAATCATTCTATA CGTGGCTAGAAGGTTTGTGTCTTGAGAAAAGAGTCTTCTATAAGCTTATATCAGGACTCCATGCCAGCAT CAATTTACATCTGTGTGCAAACTACCTTCTGGAAGAAACCTGGGGGAAACCTAGTTGGGGACCAAACATC AAGGAGTTTAGACGCCGCTTTGACCCTGTGGAAACAAAGGGGGAAGGTCCAAGGAGGCTAAAGAACCTGT ACTTTTTATACTTGATAGAGCTCCGTGCTTTGTCAAAGGTGGCCCCTTACTTTGAGCGCTCGATTGTTGA TCTCTATACTGGCAATGTGGAAGATGATGCCGACACCAAGACCCTTCTGCTCAGCATCTTTCAGGATACA AAGTCCTTTCCTATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAA AGGAAGAATTCCGGTTACATTTCAAGAACATCTCCCGGATCATGGACTGTGTTGGGTGCGATAAATGCAG ACTGTGGGGGAAACTGCAGACTCAGGGTTTAGGAACTGCCTTGAAGATCCTCTTCTCTGAAAAGGAAATC CAAAACCTTCCGGAGAACAGCCCATCCAAAGGCTTCCAGCTCACTCGGCAGGAAATCGTTGCTCTTTTAA ATGCTTTTGGAAGACTTTCTACAAGCATAAGAGAATTACAGAACTTTAAAGCGTTGTTGCAGCACAGGAG G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207470 representing BC058721 Red=Cloning site Green=Tags(s) MSPGFRRAVTGQGAAAAVQLLVTLSFLSSLVKTQVTGVLDDCLCDIDSIDKFNTYKIFPKIKKLQERDYF RYYKVNLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGRSNKYSQAANSTKELDDCEQANKLGAIN STLSNESKEAFIDWARYDDSQDHFCELDDERSPAAQYVDLLLNPERYTGYKGSSAWRVWNSIYEENCFKP RSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHASINLHLCANYLLEETWGKPSWGPNI KEFRRRFDPVETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVDLYTGNVEDDADTKTLLLSIFQDT KSFPMHFDEKSMFAGDKKGAKSLKEEFRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEI QNLPENSPSKGFQLTRQEIVALLNAFGRLSTSIRELQNFKALLQHRR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058721
ORF Size	1403 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058721, AAH58721
RefSeq Size	4255 bp
RefSeq ORF	1403 bp
Locus ID	67475
Gene Summary	Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2, PDIA3, PDIA4, PDIA6 and NXNDC12. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity). Involved in oxidative proinsulin folding in pancreatic cells, hence required for glucose homeostasis in vivo.[UniProtKB/Swiss-Prot Function]

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