Sil1 (BC016119) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1810057E01Rik; AI042831; wz
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207424 representing BC016119 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCTCAGCACCTGCCTTCAACTAGGATGGCCTCTCCAGGAATGCTGCTGGGGCTGCTGTTGACTT CCTGTTTAACTCTCTGCCTCAGCTGTCAGAACTCAAATAATTTTGCACTCACCAACCCAGAGAAGAGCAT CCACCAGGAATCAGATACAAAGGAGACCAGGGAAGAGGAGGAGCTAGACACCGAGATCCTGGAGGTGTTC CACCCAACTCAAGAGTGGCAGACCCTTCAACCAGGTCAGGCTGTTCCTGCAGGATCCCATGTGCGAATGA ACCTACAGACTGGAGTAAACGAGGTGAAGCTCCAACAAGAAGACAAATTTCAAAATAATCTGAAAGGATT TAAAAGAGGCAGAAGGCTGGACATCAACGCCAACACATACACATCTCAGGACCTCAAGAGTGCGCTGGCC AAATTCAAGGAGGGGACCGAGATGGAGAATTCAAAGGATGAACTGGCGAGACAGGCCACAGTCAAGCAGC TCTTCCGCCCCATTGAGGAGCTGAAGAAGGAATTTGATGAGCTGAACGTTGTTCTTGAGACTGACATGCA GATTATGGTTCGGCTGATAAACAAGTTCAACAGCTCCAGCTCCAGCCTAGAAGAGAAGGTTGCTGCGCTC TTTGATCTTGAGTACTATGTCCATCAGATGGATAACGCACAGGACCTGCTGTCCTTTGGTGGCCTTCAAG TGGTGATTAATGGGCTCAACAGCACAGAACCCCTGGTGAAGGAGTATGCTGCTTTTGTGCTGGGGGCCGC CTTTTCCAGCAATCCGAAGGTCCAGGTGGAAGCCATCGAAGGGGGAGCCCTGCAGAAGCTGCTGGTCATC CTGGCCACAAACCAGCCTCTCCCCGCCAAGAAGAAGGTCCTGTTTGCACTGTGCTCCCTGCTGCGCCACT TCCCCTATGCCCAGCAGCAGTTCCTGAAGCTCGGGGGCCTGCAGGTCCTCAGGAGCCTGGTACAGGAGAA GAGTGCGAAGGTACTGGCCGTGCGTGTGGTCACCCTGCTCTATGACCTGGTCACTGAGAAGATGTTTGCT GAGGAGGAGGCAGAGTTGACCCAGGATTCATCCCCAGAGAAGCTGCAGCAGTATCGCCAGGTGCAGCTCC TGCCAGGCCTGCAGGAACAGGGTTGGTGTGAGATCACCGCCCAGCTCCTGGCCCTTCCAGAGCATGATGC TCGGGAGAAGGTGCTGCAGACACTGGGTGCCCTCCTGACCACCTGCCGGGATCGCTACCGTCAGGACCTC CAGTTGAGCAGGACACTAGGGCGTCTCCAAGCAGAGTACCAAGCCCTGGCCTCCCTGGAGCTCCAAGAAG GCGAAGATGATGGCTACTTCCGGGAACTGCTGGCCTCCATCAACAGCTTGATGAAGGAACTAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207424 representing BC016119 Red=Cloning site Green=Tags(s) MAPQHLPSTRMASPGMLLGLLLTSCLTLCLSCQNSNNFALTNPEKSIHQESDTKETREEEELDTEILEVF HPTQEWQTLQPGQAVPAGSHVRMNLQTGVNEVKLQQEDKFQNNLKGFKRGRRLDINANTYTSQDLKSALA KFKEGTEMENSKDELARQATVKQLFRPIEELKKEFDELNVVLETDMQIMVRLINKFNSSSSSLEEKVAAL FDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVI LATNQPLPAKKKVLFALCSLLRHFPYAQQQFLKLGGLQVLRSLVQEKSAKVLAVRVVTLLYDLVTEKMFA EEEAELTQDSSPEKLQQYRQVQLLPGLQEQGWCEITAQLLALPEHDAREKVLQTLGALLTTCRDRYRQDL QLSRTLGRLQAEYQALASLELQEGEDDGYFRELLASINSLMKELR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016119
ORF Size	1397 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016119, AAH16119
RefSeq Size	1689 bp
RefSeq ORF	1397 bp
Locus ID	81500
Gene Summary	Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5 (By similarity).[UniProtKB/Swiss-Prot Function]

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