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Nkd2 (NM_028186) Mouse Tagged ORF Clone
CAT＃: MG207371
- TrueORF®
Nkd2 (tGFP-tagged) - Mouse naked cuticle 2 homolog (Drosophila) (Nkd2)
ORF Plasmid: DDK
"NM_028186" in other vectors (3)
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CNY 4,470.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2210403L10Rik; AW212591; mNkd2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207371 representing NM_028186
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAATTTCAGTCCAAGCACGCCGCAGCAGCCTGCAAGAGGAGAGAGAGTCCCGAAGGGGACAGCT TCGTGGTGCCTGCGTACGGGAGCGGTCGCAGAGGAGCGGAAGAAACGGACCGGCGTGCGGGCAGCGGTGT GGAACATCGCTCACGGGACAAGCAGGAGCTGCTCAATGGAGACCCTAAGGAGGGGCCTTTCTGGGACGAC AAGGGTTCCCTAGAAGTTGCGCTGCCCCCTGAGAAGTCTGAGGGCCATGAGGGCCAGGGCCAGCTCTTCA GCACAGATGATGGGGAGAAGGCAGCAAGCCGTGAGGGTCCACTGAGACTTAGCAAGAAGCACTTGAACAT TGACGCACTGCAGTGTGACGTCTCAGTGGAAGAAGACAACCGCCAAGAATGGACATTCACGTTGTATGAC TTTGACAACAGTGGGAAAGTCACCAGAGAGGATATGTCCAGCCTGATGCACACCATCTACGAGGTTGTCG ATGCCTCTGTCAATCACTCCTCCGGCAGCAGCAAGACCCTTCGAGTGAAGCTAACTGTCAGCCCTGAACC CTCCAGCAAGAAGGAATGTCCTCTCACTGGCCAAGACCGGGAGCCCACTCGTGGCAGAACAGAGATTGAG CTCACAGATGAGCCCCGAGCGGCTGACAGAAGGCTATCCGCCTACAGCAGGAAGCCCAATGCTGATCCCC AGCCCTGCTCTGTGCGAGTGCCCTACTGTGTGGATGAGAACACAGAGCGCAGAAACCACTACCTAGACCT TGCTGGCATCGAGAACTACACATCTAAGTTTGGTCCTGGGTCACCACCTGAGCAGGCCAGGCAAGAACAT CATGGCAGGGCCACACACATTCCAAGCAGGTCCCGATCACAAGAGTCGGATGCCCACGCTATACACCACC GCAGGTCTCAAGTCCTGGCTGAGCATGTCATACCAGCTAATGAGCCTGCCACCCGGGCCCTGGCTGCACA GCCCCGGCTCAAGGGGCAGGAGAAGCAGTTCCTCAGGTCTCCTAAGGGTCCAGGAAAACCTCTTGGGACA CCAGGCAGTGGCAAGCCAGGGAAAGCTCTCAGCTATTGCCTGCAGGCTGTGCCACTGCCCCAGGGTGCTC AGGATGGCCACCACCTTCCTCAGCCCCCACCACAGCCTCCACCGCAGCCCTATGGTCACAAGCGGTACCG GCAGAAAGCCAGAGAAGGCCACTCACCACTTAAGGGGCATGGCCAGCCTACCATGGTGGAGCATGAAGTG GTACGGGACCTGCCTCCCATGCTGGGGCCTGAGGGCTATGTGATGCCTGTGGTCCAGAGGCATGAACACC ACCATCACCATGAGCACCACCACCATCACCACCACCACCACTTCCACCCATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207371 representing NM_028186
Red=Cloning site Green=Tags(s) MGKFQSKHAAAACKRRESPEGDSFVVPAYGSGRRGAEETDRRAGSGVEHRSRDKQELLNGDPKEGPFWDD KGSLEVALPPEKSEGHEGQGQLFSTDDGEKAASREGPLRLSKKHLNIDALQCDVSVEEDNRQEWTFTLYD FDNSGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKKECPLTGQDREPTRGRTEIE LTDEPRAADRRLSAYSRKPNADPQPCSVRVPYCVDENTERRNHYLDLAGIENYTSKFGPGSPPEQARQEH HGRATHIPSRSRSQESDAHAIHHRRSQVLAEHVIPANEPATRALAAQPRLKGQEKQFLRSPKGPGKPLGT PGSGKPGKALSYCLQAVPLPQGAQDGHHLPQPPPQPPPQPYGHKRYRQKAREGHSPLKGHGQPTMVEHEV VRDLPPMLGPEGYVMPVVQRHEHHHHHEHHHHHHHHHFHPS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028186 |
| ORF Size | 1383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028186.2, NP_082462.2 |
| RefSeq Size | 2399 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 72293 |
| UniProt ID | Q8VE28 |
| Gene Summary | Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR207371 | Nkd2 (Myc-DDK-tagged) - Mouse naked cuticle 2 homolog (Drosophila) (Nkd2) |
CNY 3,656.00 |
|
| MR207371L3 | Lenti ORF clone of Nkd2 (Myc-DDK-tagged) - Mouse naked cuticle 2 homolog (Drosophila) (Nkd2) |
CNY 5,990.00 |
|
| MR207371L4 | Lenti ORF clone of Nkd2 (mGFP-tagged) - Mouse naked cuticle 2 homolog (Drosophila) (Nkd2) |
CNY 5,990.00 |
