Rarg (BC012923) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	RARD, Nr1b3, RARgamma2, MGC11555, MGC18523
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207310 representing BC012923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCAATAAGGAGAGACTCTTTGCGCCCGGTGCCCTGGGGCCTGGATCTGGTTACCCAGGAGCAG GCTTCCCATTCGCCTTCCCAGGTGCACTCAGAGGGTCGCCACCATTTGAGATGCTGAGCCCTAGCTTCCG GGGCCTGGGCCAGCCTGACCTCCCCAAGGAGATGGCTTCTCTCTCGGTGGAGACACAGAGCACCAGCTCG GAGGAGATGGTACCCAGCTCTCCCTCACCCCCACCACCTCCTCGGGTCTATAAGCCATGCTTTGTATGCA ATGACAAGTCTTCTGGCTACCACTATGGGGTCAGCTCCTGTGAAGGCTGCAAGGGCTTCTTCAGACGCAG CATTCAGAAAAACATGGTGTATACATGTCACCGTGACAAAAACTGTATCATCAACAAGGTCACCAGAAAT CGATGCCAGTACTGCAGGCTACAAAAGTGTTTCGAAGTGGGCATGTCCAAGGAAGCTGTAAGGAACGATC GAAACAAGAAGAAAAAGGAGGTAAAAGAGGAGGGCTCGCCCGACAGCTATGAACTGAGTCCACAGTTAGA GGAACTCATCACCAAGGTCAGCAAAGCCCACCAGGAGACTTTTCCCTCACTCTGCCAGCTGGGCAAGTAC ACCACGAACTCCAGTGCAGATCACCGGGTGCAGCTGGACCTGGGGCTGTGGGACAAGTTCAGCGAGCTGG CCACCAAATGCATCATCAAGATTGTGGAGTTTGCGAAGCGGCTGCCTGGTTTTACAGGGCTCAGCATTGC CGACCAGATCACGCTGCTCAAGGCTGCTTGTCTGGACATCCTAATGCTGCGGATCTGTACAAGGTATACC CCAGAGCAGGACACTATGACATTCTCGGATGGGCTGACCCTGAACCGAACCCAGATGCACAATGCTGGCT TTGGGCCCCTTACAGACCTCGTCTTTGCCTTTGCCGGGCAGCTGCTGCCCCTGGAGATGGATGACACCGA GACTGGGCTACTTAGTGCTATCTGCCTCATCTGTGGAGACCGAATGGACCTGGAAGAGCCCGAGAAGGTG GACAAGCTGCAGGAGCCCCTGCTGGAAGCCCTGAGGCTCTATGCCCGGCGACGGAGACCCAGCCAACCCT ACATGTTCCCAAGGATGCTGATGAAAATCACCGACCTCCGGGGCATCAGCACTAAGGGAGCAGAAAGGGC TATAACCCTGAAGATGGAGATTCCAGGCCCGATGCCACCCCTGATCCGAGAGATGCTGGAGAACCCGGAG ATGTTTGAGGACGACTCCTCGAAGCCTGGCCCCCACCCCAAGGCTTCCAGTGAGGACGAAGCTCCAGGGG GCCAGGGCAAAAGGGGCCAAAGTCCCCAACCTGACCAGGGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207310 representing BC012923 Red=Cloning site Green=Tags(s) MATNKERLFAPGALGPGSGYPGAGFPFAFPGALRGSPPFEMLSPSFRGLGQPDLPKEMASLSVETQSTSS EEMVPSSPSPPPPPRVYKPCFVCNDKSSGYHYGVSSCEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRN RCQYCRLQKCFEVGMSKEAVRNDRNKKKKEVKEEGSPDSYELSPQLEELITKVSKAHQETFPSLCQLGKY TTNSSADHRVQLDLGLWDKFSELATKCIIKIVEFAKRLPGFTGLSIADQITLLKAACLDILMLRICTRYT PEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFAGQLLPLEMDDTETGLLSAICLICGDRMDLEEPEKV DKLQEPLLEALRLYARRRRPSQPYMFPRMLMKITDLRGISTKGAERAITLKMEIPGPMPPLIREMLENPE MFEDDSSKPGPHPKASSEDEAPGGQGKRGQSPQPDQGP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC012923
ORF Size	1376 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012923, AAH12923
RefSeq Size	2381 bp
RefSeq ORF	1376 bp
Locus ID	19411
Gene Summary	Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (By similarity). Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.[UniProtKB/Swiss-Prot Function]

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