Atg4c (BC058981) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Apg4-C, Atg4cl
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207307 representing BC058981 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTTCAGGAACAGATGAAGTTGACAAGCTAAAAACTAAATTTATATCTGCTTGGAACAATATGA AATATAGTTGGGTGTTGAAAACAAAGACATATTTTAGTAGAAATTCACCTGTTTTATTGCTTGGAAAATG TTATCATTTTAAATATGAAGATGAAAGCAAGATGTTGCCTGCAAGATCAGGATGTGCCATTGAAGATCAT GTAATTGCAGGGAATGTGGAAGAGTTCCGTAAGGATTTCATTTCTAGATTATGGCTGACCTACAGGGAAG AATTTCCTCAAATCGAAGCTTCAGCCTTGACGACAGACTGTGGCTGGGGCTGCACGCTGCGGACCGGCCA GATGCTGCTGGCTCAGGGACTCATACTCCATTTTCTCGGTAGAGCTTGGACCTGGCCTGATGCGCTGCAC ATTGAGAACGCAGACTCTGACTCATGGACTTCCAACACTGTCAAAAAGTTTACTGCATCGTTTGAAGCTT CACTTTCGGGGGACAGAGAACTCAGAACTCCAGCAGTTTCTCTGAAGGAAACATCTGGGAAGTGTCCCGA TGACCACGCAGTGCGGAATGAGGCTTATCACAGGAAGATCATCTCTTGGTTTGGAGATTCCCCCGTGGCC GTCTTTGGCTTACATCGTCTGATAGAGTTTGGGAAGAAGTCTGGGAAAAAAGCTGGCGATTGGTATGGAC CAGCGGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCCGGATTTGCAAGGGCTAAC TATTTATGTTGCCCAGGACTGTACAGTTTACAATTCTGACGTGATTGATAAGCAGACTGACTCCGTGACA GCTGGAGATGCACGTGACAAGGCTGTCATTATTTTAGTTCCTGTTAGACTCGGTGGAGAAAGGACCAATA CCGACTATCTGGAGTTTGTGAAGGGTGTTTTAAGCCTTGAATACTGTGTAGGTATCATTGGCGGCAAACC TAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATGGATCCTCATTACTGCCAG TCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTCGAGACATTCCACTGTCCATCTCCTAAAAAGATGT CATTTCGAAAAATGGATCCTAGTTGTACGATAGGATTTTACTGTCGAAATGTCCAGGACTTTGAACGAGC GTCTGAAGAAATCACTAAGATGTTGAAAATTTCCTCTAAGGAGAAATATCCACTATTTACTTTTGTAAAT GGCCATTCCAAAGACTTTGATTTTACATCTACCGCAGCCAGTGAAGAAGACCTTTTCTCAGAAGATGAAA GGAAGAATTTCAAAAGATTTAGCACAGAAGAGTTTGTCCTCCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207307 representing BC058981 Red=Cloning site Green=Tags(s) MEASGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDESKMLPARSGCAIEDH VIAGNVEEFRKDFISRLWLTYREEFPQIEASALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALH IENADSDSWTSNTVKKFTASFEASLSGDRELRTPAVSLKETSGKCPDDHAVRNEAYHRKIISWFGDSPVA VFGLHRLIEFGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGLTIYVAQDCTVYNSDVIDKQTDSVT AGDARDKAVIILVPVRLGGERTNTDYLEFVKGVLSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQ SFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFERASEEITKMLKISSKEKYPLFTFVN GHSKDFDFTSTAASEEDLFSEDERKNFKRFSTEEFVLL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058981
ORF Size	1376 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058981, AAH58981
RefSeq Size	3232 bp
RefSeq ORF	1376 bp
Locus ID	242557
Gene Summary	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity). Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation.[UniProtKB/Swiss-Prot Function]

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