Prune1 (NM_173347) Mouse Tagged ORF Clone
CAT#: MG207254
- TrueORF®
Prune (tGFP-tagged) - Mouse prune homolog (Drosophila) (Prune)
ORF Plasmid: DDK
"NM_173347" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 9230112O05Rik; C130058A12; DRES-17; HTCD37; Prune-M1; PRUNEM1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207254 representing NM_173347
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTACCTGCAGGACTGTCGAGCCGCTTTGCAGGATTCCAGGCCTCTACATGTCGTGCTGGGAA ATGAAGCCTGCGACTTAGACTCCATGGTGTCTGCTCTCGCCTTGGCCTTCTACCTAACAAAGACGTCTGA AGCTGAAGACATCTTTATACCAGTTTTAAATATAAAGCGTTCTGAGCTTCCTCTGCGCGGGGACAATGTC TTCTTTCTCCAGGAGGTTAAGATTCCAGAGCCAGCCCTGATCTTCCGGGACGAGATTGACCTCCTAGCCC TGCACCAGGCTGGCCAGCTCACCCTCATCCTTGTTGACCACCATATTTTACCCAAAAGCGATGCAGCTCT GGAGGAGGCCGTAGCAGAGGTGCTGGACCACCGACCAATTGAGCAGAAATACTGTCCTCCCTGTCACGTT TCAGTTGAGCTGGTGGGGTCCTGTGCCACCCTGGTGACAGAGAGAATCCTGCAGGGGGCACCAGAGACCT TGGACAGACAAACTGCAGCCCTACTGCATGGAACCATCATCCTGGACTGTGTCAACATGGATACCAACAT TGGGAAGGCAACCCCAAAGGACAGCAAATATGTGGAAGAACTGGAAGCCCTTTTCCCAGATCTGCCCAAA AGAAAGGACATATTTGATTCCCTGCAGAAGGCCAAGTTTGACGTCTCAGGACTGACCACTGAACAGATGC TCAGAAAGGACCAGAAGACAGTCTACAGACAGGGCACCAAGGTGGCAATCAGTGCAATCTACATGGACTT GAAGGCCTTTCTGCAGCGGACAGACCTCTTCACAGATCTCAGTTCTTTCTGCCATGATCACAGCTATGAT GCCCTGGTTGCCATGACTATCTTTTTCAACACTCAAAATGAGCCAGTACGGCAATTAGCTATCTTCTGTC CACACGAGGCACTTCGAATGACAATCTGTGGCATCCTGGAACGATCCACCTCCCCACCCCTGAAGCTGAC ACCTATCCCAAGCACCTCCCCTAACCTCCAGGCCTATCACCAAGGCAACACCCAGGTCTCTCGGAAGAAG CTGCTGCCTGTGCTCCAGGAAGCCCTCTCCGCATATCTTGACTCAGCGAAGATGGCTTCGGGGCAGTCTG AGGTAGCTGTGGGTATGTCTAGGGAGCAGGTGGACAAGGACTTGGACAAGGCAAGTAACTCCCTGATTTC TGGGCTGAGTCAAGATGAGGAAGACCCTCCGCTGCCACCCACGCCTATGAACAGCTTGGTGGATGAGTGC CCTCTGGATCAGGGGCTGCCTAAGTTCTCCGCTGAGGCTGTCTTTGAGAAGTGCAGTCAGATCTCACTAT CCCAGTCTGCCAGAGCCTGCACATCAAATAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207254 representing NM_173347
Red=Cloning site Green=Tags(s) MEDYLQDCRAALQDSRPLHVVLGNEACDLDSMVSALALAFYLTKTSEAEDIFIPVLNIKRSELPLRGDNV FFLQEVKIPEPALIFRDEIDLLALHQAGQLTLILVDHHILPKSDAALEEAVAEVLDHRPIEQKYCPPCHV SVELVGSCATLVTERILQGAPETLDRQTAALLHGTIILDCVNMDTNIGKATPKDSKYVEELEALFPDLPK RKDIFDSLQKAKFDVSGLTTEQMLRKDQKTVYRQGTKVAISAIYMDLKAFLQRTDLFTDLSSFCHDHSYD ALVAMTIFFNTQNEPVRQLAIFCPHEALRMTICGILERSTSPPLKLTPIPSTSPNLQAYHQGNTQVSRKK LLPVLQEALSAYLDSAKMASGQSEVAVGMSREQVDKDLDKASNSLISGLSQDEEDPPLPPTPMNSLVDEC PLDQGLPKFSAEAVFEKCSQISLSQSARACTSNK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_173347 |
ORF Size | 1362 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173347.2, NP_775482.1 |
RefSeq Size | 3160 bp |
RefSeq ORF | 1365 bp |
Locus ID | 229589 |
UniProt ID | Q8BIW1 |
Gene Summary | Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205277 | Prune (untagged) - Mouse prune homolog (Drosophila) (Prune), (10ug) |
CNY 3,656.00 |
|
MR207254 | Prune (Myc-DDK-tagged) - Mouse prune homolog (Drosophila) (Prune) |
CNY 3,656.00 |
|
MR207254L3 | Lenti ORF clone of Prune (Myc-DDK-tagged) - Mouse prune homolog (Drosophila) (Prune) |
CNY 5,230.00 |
|
MR207254L4 | Lenti ORF clone of Prune (mGFP-tagged) - Mouse prune homolog (Drosophila) (Prune) |
CNY 5,230.00 |