Dnaja3 (BC027240) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1810053A11Rik, Tid1l, Tid-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207237 representing BC027240 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGTGGTGTTCCCCACGCTGGTTGAGGGTGGCAGTGGGGACTCCCCGGCTGCCGGCTGCAGCGG GGAGAGGGGTCCAGCAGCCCCAGGGGGGCGTAGTGGCAACATCCCTGTGTCGCAAGCTGTGCGTCTCTGC CTTCGGGCTTTCCATGGGCGCTCACGGCCCCCGGGCGCTGCTGACATTGAGACCCGGTGTCCGCCTCACA GGAACAAAAAGTTTCCCTTTTGTTTGTACCACCTCTTTCCACACAAGTGCTTCTTTGGCCAAAGATGATT ATTACCAGATCCTGGGAGTGCCCCGAAATGCCAGCCAGAAAGATATCAAGAAAGCCTATTATCAGCTTGC CAAGAAATATCATCCAGACACAAATAAGGATGATCCCAAAGCCAAGGAGAAGTTTTCCCAGCTGGCAGAA GCTTATGAGGTGTTGAGCGATGAAGTGAAGAGGAAGCAGTATGACGCTTACGGCTCTGCTGGCTTTGACC CTGGCACCAGCAGCTCTGGGCAGGGCTACTGGAGAGGAGGTCCCTCTGTTGACCCCGAGGAGTTGTTCAG GAAGATCTTTGGGGAGTTCTCATCTTCTCCTTTTGGTGATTTCCAGAATGTGTTTGATCAGCCTCAGGAA TACATCATGGAATTGACATTCAATCAAGCTGCCAAGGGTGTCAACAAAGAGTTCACTGTGAACATTATGG ATACCTGTGAGCGCTGTGACGGCAAGGGGAACGAGCCTGGAACCAAAGTGCAGCATTGCCATTACTGTGG CGGCTCGGGCATGGAAACTATCAATACAGGGCCTTTTGTGATGCGTTCCACATGTCGGAGATGTGGTGGC CGGGGCTCCATCATCACAAATCCCTGTGTGGTCTGCAGGGGAGCAGGACAAGCCAAGCAGAAGAAGCGCG TGACAATTCCTGTGCCTGCAGGAGTTGAAGATGGTCAGACTGTAAGGATGCCAGTGGGAAAACGAGAAAT TTTTGTCACATTCAGGGTGCAGAAAAGCCCTGTGTTCCGGAGGGACGGTGCAGACATCCACTCGGACCTC TTTATTTCAATAGCTCAAGCTATTCTTGGGGGCACAGCCAAAGCCCAGGGCCTGTATGAGACAATCAATG TGACGATCCCTGCAGGGATTCAGACAGACCAGAAGATTCGTTTGACTGGAAAAGGCATCCCCCGGATTAA TAGCTATGGCTATGGTGACCACTACATTCACATCAAGATCAGAGTTCCAAAGAGGCTGAGCAGTAGGCAG CAGAACCTGATCCTGAGCTATGCCGAGGATGAGACTGACGTGGAGGGGACGGTTAATGGAGTCACCCACA CAAGCACTGGAAAGCGGTCAACTGGAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207237 representing BC027240 Red=Cloning site Green=Tags(s) MAAWCSPRWLRVAVGTPRLPAAAGRGVQQPQGGVVATSLCRKLCVSAFGLSMGAHGPRALLTLRPGVRLT GTKSFPFVCTTSFHTSASLAKDDYYQILGVPRNASQKDIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAE AYEVLSDEVKRKQYDAYGSAGFDPGTSSSGQGYWRGGPSVDPEELFRKIFGEFSSSPFGDFQNVFDQPQE YIMELTFNQAAKGVNKEFTVNIMDTCERCDGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGG RGSIITNPCVVCRGAGQAKQKKRVTIPVPAGVEDGQTVRMPVGKREIFVTFRVQKSPVFRRDGADIHSDL FISIAQAILGGTAKAQGLYETINVTIPAGIQTDQKIRLTGKGIPRINSYGYGDHYIHIKIRVPKRLSSRQ QNLILSYAEDETDVEGTVNGVTHTSTGKRSTGN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027240
ORF Size	1359 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027240, AAH27240
RefSeq Size	2545 bp
RefSeq ORF	1361 bp
Locus ID	83945
Gene Summary	Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.[UniProtKB/Swiss-Prot Function]

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