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Sctr (BC089355) Mouse Tagged ORF Clone
CAT＃: MG207131
- TrueORF®
Sctr (tGFP-tagged) - Mouse secretin receptor (cDNA clone MGC:102218 IMAGE:30551620)
ORF Plasmid: DDK
"BC089355" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6530402O03Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207131 representing BC089355
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGCACCATGAGCCCCCGGCTGTCACTGCTTCTGCTGTGGCTGCTGCTGCTCATCAATGCAGCGC ACCCTGTTGGAGCTCTGCCCAGATTGTGTGATGTGCGGCGTGTGCTGCTCGAGGAACGGGCCGAGTGCCT GCGGGAACTCTCCGAAGAGAAGAAGGCGCTGGGCCCGAAGACAGCATCAGGCTGTGAGAGGTTTTGGGAC AACATGAGCTGCTGGCCCTCCTCGGCACTGGCACAGACAGTGGAAGTGCCATGTCCGAAGTTCCTGAGGA TGTTCTCTGGCAGAAATGGGTCTCTGTTCCGGAACTGCACAAAGGATGGCTGGTCAGAAACCTTCCCCAG GCCTGACCTGGCCTGTGGGGTCAACATGAATGGCTCCTTTAATGAGAGACGGCATGCATACCTGCTGAAG CTCAAGGTCATGTACACTGTAGGCTACAGTTCCTCCTTGGCCATGCTCCTGGTCGCCCTCAGCATCCTGT GCTCTTTCCGGAGGCTGCACTGCACTCGCAACTACATCCACATGCACCTGTTTGTGTCCTTCATCCTTCG CGCCCTGTCCAACTTCATCAAGGACGCCGTACTCTTCCCCGCAGATGACGTCACCTACTGTGATGCCCAC AGGGCAGGCTGCAAGCTGGTCATGATCTTCTTCCAGTACTGCATCATGGCCAACTACGCGTGGCTGCTGG TGGAGGGCCTCTATCTTCACACGCTCCTGGCCATCTCCTTCTTCTCAGAAAGGAAGTGCCTGCAGGCATT TGTGCTCTTCGGATGGGGTTCTCCAGCCATTTTTGTTGCTTTGTGGGCTGTCACCAGACACTTTCTGGAG GACTTTGGATGCTGGGACATCAACTCCAACGCTTCCATCTGGTGGGTCATTCGAGGGCCTGTGATTCTGT CCATCGTGATCAATTTCATCTTTTTCATAAACATTCTAAGAATCCTGATGAGAAAACTTAGAACTCAAGA AACAAGAGGAAATGAAACACACCATTATAAGCGCCTGGCCAAGTCCACCCTCCTGCTGATCCCTCTCTTT GGCATCCACTACATTGTCTTCGCCTTCTCCCCAGAGGGTGCCATGGAGGTCCAGCTGTTCTTTGAATTGG CCCTGGGCTCCTTCCAGGGGCTGGTGGTAGCTGTCCTTTACTGCTTCCTCAATGGTGAGCTGGAAGTTCA GAAAAAATGGCGCCAGTGGCACCTCCAAGAGTTCCCGCTGCGCCCTGTGGCCCTCAGCAACTCCTTCAGC AACGCCACCAACGGCCCCACCCACAGCACCAAGGCTGGCACCTCTGAGCAGAGCCGGAGCATCCCCGGGG CCAATGTCATC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207131 representing BC089355
Red=Cloning site Green=Tags(s) MLSTMSPRLSLLLLWLLLLINAAHPVGALPRLCDVRRVLLEERAECLRELSEEKKALGPKTASGCERFWD NMSCWPSSALAQTVEVPCPKFLRMFSGRNGSLFRNCTKDGWSETFPRPDLACGVNMNGSFNERRHAYLLK LKVMYTVGYSSSLAMLLVALSILCSFRRLHCTRNYIHMHLFVSFILRALSNFIKDAVLFPADDVTYCDAH RAGCKLVMIFFQYCIMANYAWLLVEGLYLHTLLAISFFSERKCLQAFVLFGWGSPAIFVALWAVTRHFLE DFGCWDINSNASIWWVIRGPVILSIVINFIFFINILRILMRKLRTQETRGNETHHYKRLAKSTLLLIPLF GIHYIVFAFSPEGAMEVQLFFELALGSFQGLVVAVLYCFLNGELEVQKKWRQWHLQEFPLRPVALSNSFS NATNGPTHSTKAGTSEQSRSIPGANVI SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC089355 |
| ORF Size | 1343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC089355, AAH89355 |
| RefSeq Size | 1834 bp |
| RefSeq ORF | 1343 bp |
| Locus ID | 319229 |
| Gene Summary | Receptor for secretin (SCT), which is involved in different processes such as regulation of the pH of the duodenal content, food intake and water homeostasis (PubMed:20927047, PubMed:24273196, PubMed:30449620). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (PubMed:30449620). Upon binding to secretin, regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas (By similarity). In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation (PubMed:30449620). Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation (PubMed:30449620). Also able to stimulate lipolysis in white adipocytes (PubMed:24273196). Also plays an important role in cellular osmoregulation by regulating renal water reabsorption (PubMed:17283064). Also plays a role in the central nervous system: required for synaptic plasticity (PubMed:17008357).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218676 | Sctr (untagged) - Mouse secretin receptor (cDNA clone MGC:102218 IMAGE:30551620), (10ug) |
CNY 3,656.00 |
|
| MR207131 | Sctr (Myc-DDK-tagged) - Mouse secretin receptor (cDNA clone MGC:102218 IMAGE:30551620) |
CNY 3,656.00 |
|
| MR207131L3 | Lenti ORF clone of Sctr (Myc-DDK-tagged) - Mouse secretin receptor (cDNA clone MGC:102218 IMAGE:30551620) |
CNY 4,470.00 |
|
| MR207131L4 | Lenti ORF clone of Sctr (mGFP-tagged) - Mouse secretin receptor (cDNA clone MGC:102218 IMAGE:30551620) |
CNY 4,470.00 |
